Rickettsioses have an epidemiological importance that includes pathogens, vectors, and hosts. The dog tick Rhipicephalus sanguineus the camel Hyalomma dromedarii play important roles as vectors reservoirs of Rickettsiae. aim this study was to determine prevalence Rickettsiae in ixodid ticks species infesting dogs camels Egypt, addition to, morphological molecular identification R. H. dromedarii.A total 601 104 ticks' specimens were collected from camels, respectively, Cairo, Giza Sinai...

Background and Aim: Q fever Coxiella burnetii is a worldwide zoonotic disease, C. was detected in mammals ticks.Ticks play an important role the spread of environment.Therefore, aims this study were to detect camels ixodid ticks by molecular tools identification Hyalomma dromedarii excavatum using immunological assays. Materials Methods:A total 113 blood samples from 190 adult investigated for infection with polymerase chain reaction (PCR) sequencing targeting IS30A spacer.The two tick...

Vector Borne Diseases (VBDs) are considered emerging and re-emerging diseases that represent a global burden. The aim of this study was to explore characterize vector-borne pathogens in different domestic animal hosts Egypt. A total 557 blood samples were collected from animals using convenience sampling strategy (203 dogs, 149 camels, 88 cattle, 26 buffaloes, 58 sheep 33 goats). All tested for multiple quantitative PCR standard coupled with sequencing. We identified Theileria annulata...

Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports canine feline diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs Asir (70 44 cats) Riyadh (74 dogs), Arabia. The presence Anaplasma spp., Bartonella hemotropic Mycoplasma Babesia Hepatozoon spp. was detected using a multiplex tandem real-time PCR. PCR-positive further examined with specific conventional PCR followed by sequencing. tested negative for all while...

Piroplasmosis and anaplasmosis are serious tick-borne diseases (TBDs) that concerning for the public animal health. This study aimed to detect molecular prevalence epidemiological risk factors of Piroplasma Anaplasma species in hosts their associated ticks Egypt. A total 234 blood samples 95 adult were collected from (112 cattle, 38 sheep, 28 goats, 26 buffaloes, 22 donkeys, 8 horses) six provinces Egypt (AL-Faiyum, AL-Giza, Beni-Suef, Al-Minufia, Al-Beheira, Matruh). Blood tick investigated...

Abstract Leishmania major and tropica cause cutaneous leishmaniasis in humans dogs several parts of the world, with a large number cases recorded Middle East. However, when they occur sympatry, role each species epidemiology (CL) is not clear. To assess frequency to identify that infect stray Riyadh Al-Qaseem (Saudi Arabia), 311 27 human patients who were suspected for infection examined CL by nested polymerase chain reaction (nPCR). Seven (25.9%) out scored positive spp. (i.e., L. five from...

Cryptosporidiosis is a leading cause of diarrheal disease worldwide and an animal public health burden. This study aimed to evaluate the protective potential affinity-purified Cryptosporidium parvum oocyst antigen as vaccine candidate according fecal shedding, humoral cellular immune responses, histopathological changes, number parasite developmental stages in ileal hepatic tissues. We isolated oocysts from naturally infected buffalo calves identified them molecularly C. isolates (GenBank:...

Q fever is a zoonotic disease caused by Coxiella burnetii. Cattle, sheep, and goat are the main reservoir of C. In Egypt, epidemiological data about burnetii in camels limited. Therefore, current study was conducted to identify infection different molecular tools estimate its seropositivity through detection anti-C. antibodies camel sera.Blood samples were collected 112 from Giza Cairo Provinces, Egypt. All blood screened trans-quantitative polymerase chain reaction (trans-qPCR) for positive...

Rickettsioses including their pathogens, vectors, and hosts have an epidemiological importance zoonotic importance. The objective of the present article was to define prevalence genotypic properties Rickettsia in camels ticks Egypt. Sixty one blood samples 99 adult were taken from camel Cairo, Giza Sinai, during a period extending 2013 2014. Based on morphological identification both male female tick specimens, 91.9 % collected Hyalomma dromedarii. using Gimenez staining technique PCR 0 41...

Knowledge of molecular identification tick-borne pathogens in camels Saudi Arabia is very limited; few epidemiological studies have been under taken. This study was to detect Anaplasma spp. and Piroplasma from Asir Province, Arabia. A total 150 blood samples were collected Province investigated by polymerase chain reaction (PCR) that targeted 18S rRNA 23S the DNA spp., respectively. The positive for assayed again PCR targeting 16S rRNA. All free infection. Three (2%) found be infection...

C RYPTOSPORIDIOSIS is a significant disease that causes diarrhea in humans and animals with relatively high morbidity mortality.The present study aimed to adopt purified potent antigen for the accurate diagnosis of cryptosporidiosis.A total 278 animal hosts (60 newborn calves 218 buffaloes) were used current study.Sixty fecal samples collected from new-born aged less than one month raised Beni-Suef Qalyubia governorates.The examined under microscope after modified Ziehl-Neelsen staining,...

Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role pathology of normal aging. Vimentin cytokeratin are cytoskeletal intermediate filaments that often used as indirect indices tissue injury. The aim study was to clarify age-related alterations structure function relationship between oxidative/antioxidative stress markers (vimentin cytokeratin) well vascular endothelial growth factor (VEGF) during aging were elucidated. Finally, Nigella sativa...

Background: Rickettsioses are intracellular bacterial infectious diseases causing high morbidity and sometime mortality with public health importance. Aim: The aim of present study was to detect rickettsiae in dogs horses Cairo, Egypt by using Polymerase Chain Reaction (PCR) targeting OmpA gene. Methods: A total number 200 blood samples (100 from 100 horses) were collected Cairo province, Egypt. All investigated PCR gene the rickettsial infection. Furthermore, hematological examination for...

Abstract Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging transmitted by arthropods most of these zoonotic concern. This study was designed to screen EVBD in equines their vectors using molecular analyses identify MALDI-TOF techniques. Methods A total 335 blood samples were collected from apparently healthy (320 horses 15 donkeys) Cairo Beni-Suef provinces Egypt. 166 (105 sucking flies 61 ticks) the same animals. techniques used confirm findings...

Endothelial dysfunction is evident in systemic lupus erythematosus (SLE). Pro-inflammatory adipokines are involved endothelial derangement and premature atherosclerosis, particularly nephritis (LN). This study aimed to investigate the impact of LN on function by estimating serum levels adiponectin, leptin, visfatin, homeostatic model assessment-insulin resistance (HOMA-IR) calculating flow-mediated dilatation (FMD) brachial artery. a case-control which 190 patients who were fulfilling...

Abstract Background and aim: Babesiosis anaplasmosis are tick-borne diseases that affect the health of cattle may be also responsible for remarkable economic losses. This study aimed to detect presence causative agents babesiosis among in different provinces Saudi Arabia characterise their species genetically. Methodology: A total 362 blood samples were taken from four regions (Riyadh, Al-Kharj, Al-Hasa Al-Qassim) molecularly screened by polymerase chain reaction (PCR) partial 18S rRNA 23S...

Abstract Background: Leishmania major and tropica cause cutaneous leishmaniasis in humans dogs several parts of the world, with a large number cases recorded Middle East. However, when occurring sympatry, role each species epidemiology (CL) is not clear. Methods: To determine prevalence to identify that infects stray Riyadh Al-Qassim (Saudi Arabia), 311 27 human patients, suspected for Leishmania, were examined CL by nested PCR (nPCR). Results: Nested (nPCR) detected seven patients (25.9%)...

Equine filariosis (EF) is a neglected vector-borne disease caused by nematode species belonging to the Onchocercidae and Setariidae families. Aside from their zoonotic potential, some are responsible for serious health problems in equids worldwide, leading significant economic difficulties. Here, we molecularly investigated equine blood samples (320 horses 109 donkeys Egypt) four adult worms isolated peritoneal cavity of 5 out 94 slaughtered donkeys. In addition, quantitative enzyme-linked...