A5090795358- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Electrowetting and Microfluidic Technologies
- Orbital Angular Momentum in Optics
- 3D Printing in Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lipid Membrane Structure and Behavior
- Photochromic and Fluorescence Chemistry
- Modular Robots and Swarm Intelligence
- Photoreceptor and optogenetics research
- Micro and Nano Robotics
- Photonic and Optical Devices
- Nanofabrication and Lithography Techniques
- Carbon Nanotubes in Composites
- Surgical Simulation and Training
- Retinal and Macular Surgery
- Electrohydrodynamics and Fluid Dynamics
- Mechanical and Optical Resonators
- Advanced MEMS and NEMS Technologies
- Cellular Mechanics and Interactions
- Advanced Sensor and Energy Harvesting Materials
- Nanopore and Nanochannel Transport Studies
- Neuroscience and Neural Engineering
Nagoya University
2015-2024
The University of Tokyo
2021
United Biomedical (United States)
2016
Tohoku University
2006-2010
A novel approach appropriate for rapid separation and immobilization of a single cell by concomitantly utilizing laser manipulation locally thermosensitive hydrogelation is proposed in this paper. We employed beam as optical tweezers separating target locating it adjacent to fabricated, transparent micro heater. Simultaneously, the immobilized or partially entrapped heating hydrogel with The state can be switched from sol gel controlling temperature through cooling After other unwanted cells...
ABSTRACT Photoautotrophic bacteria have developed mechanisms to maintain K + homeostasis under conditions of changing ionic concentrations in the environment. Synechocystis sp. strain PCC 6803 contains genes encoding a well-characterized Ktr-type uptake transporter (Ktr) and putative ATP-dependent specific for (Kdp). The contributions each these transport systems cellular not yet been defined conclusively. To verify functionality Kdp, kdp were expressed Escherichia coli , where Kdp conferred...
Influenza virus infection can result in changes the cellular ion levels at 2–3 hours post-infection. More H+ is produced by glycolysis, and viral M2 proton channel also plays a role capture release of during both entry egress. Then cells might regulate intracellular pH increasing export from compartment. Increased could lead indirectly to increased extracellular acidity. To detect virus-infected uninfected cells, sensors were synthesized using polystyrene beads (1μm) containing Rhodamine B...
In noncontact cell manipulation with optical tweezers, we need to use microtools avoid damages the cells by direct laser irradiation. When manipulate complex structures such as microtools, a multi-beam tweezers which can multiple objects is suitable; however speed or trapping number are not so good. Therefore, developed an integrated using time-shared scanning method for high and generalized phase contrast lot of trapping. Then, proposed suits then designed shape made SU-8. A new fabrication...
We developed an active virus filter (AVF) that enables enrichment for single infection, by using insulator-based dielectrophoresis (iDEP). A 3D-constricted flow channel design enabled the production of iDEP force in microfluidic chip. a chip with multiple filters (AVFs) was more accurate and faster than AVF, improved efficiency trapping. utilized maskless photolithography to achieve precise 3D gray-scale exposure required fabrication constricted channel. Influenza (A PR/8) enriched negative...
This paper demonstrates local pH measurement in a microchip using pH-sensing gel-microbead. To achieve this, the gel-microbead made of hydrophilic photo-crosslinkable resin was functionalized with indicator bromothymol blue (BTB). The primary constituent this is poly(ethylene glycol). Gel-microbeads impregnated BTB were obtained by stirring mixture solution, which composed resin, BTB, and an electrolyte solution. polymerized UV illumination. can be manipulated optical tweezers to adhere...
This paper reports transportation of the target microbe by laser trapped microtools with minimum irradiation to target. The size a microtool (MT) is around micrometer. MTs are manipulated focused under microscope manipulate microbe. Here we propose pinpoint injection method at desired location in microchamber, which filled liquid. At first, classified four categories. employed new install inside microchamber. We developed MT holding chip MTs. were injected and successfully scanning...
The moderately halotolerant cyanobacterium Synechocystis sp. strain PCC 6803 contains a plasma membrane aquaporin, AqpZ. We previously reported that AqpZ plays role in glucose metabolism under photomixotrophic growth conditions, suggesting involvement of cytosolic osmolarity homeostasis. To further elucidate the physiological AqpZ, we have studied its gene expression profile and function Synechocystis. level aqpZ was regulated by circadian clock. activity insensitive to mercury Xenopus...
Collective migration of epithelial cells is a fundamental process in multicellular pattern formation. As they expand their territory, are exposed to various physical forces generated by cell-cell interactions and the surrounding microenvironment. While stress applied neighbouring has been well studied, little known about how niches that surround spatio-temporally remodelled regulate collective cell Here, we analysed extracellular matrix (ECM) alters resistance force exerted on so can...
Abstract
A novel separation method for random screening of target cells from a large heterogeneous population by using local photo-polymerization is developed. photo-crosslinkable resin solution mixed with the sample liquid and we controlled state sol to gel irradiating near ultraviolet (UV) light mercury lamp He–Cd laser cell. We applied three types immobilization methods such as direct method, caging position control method. The selected cell immobilized in cured directly or inside cage resin. In...
We have assembled thermal probes with single carbon nanotubes (CNTs) for sensing temperature distribution in local area. The coefficient of resistance the shows positive characteristics. feasibility CNT scanning area is investigated nanometer order. implemented measurement and calibration nanoheater, demonstrated them to be novel sensors.
On-chip environment measurement is an important technique for control ambient conditions of cells and monitoring cell activities. However, integration many sensors in the chip has difficulties such as position interconnection sensors. We developed hydrogel microsensors made photocrosslinkable resin impregnating with indicators on-chip measurement. This sensor can be used both area 3-D sensing by shaping UV ray patterning. requires no interconnections since environmental information detected...
The present study was designed to investigate the local microenvironment of octacalcium phosphate in a granule form upon biomolecules adsorption utilizing an indicator-immobilized-gel-sheet for measuring pH. We previously showed that enhances bone regeneration during its progressive hydrolysis into hydroxyapatite if implanted defects. gel-sheet made from photocrosslinkable prepolymer solution, which can easily immobilize pH indicator (bromothymol blue; BTB) hydrogel. mounted on biochip...
We developed fabrication and manipulation of hybrid nanorobots by 3D nano exposure optical tweezers. Hybrid nanorobot is composed photoresist silicon nanowire. The robot fabricated femtosecond laser connected to the glass substrate a micropillar. processing resolutions are 270 nm (line width) 600 (thickness), respectively. released cutting pillar ablation with laser. Release time within four minutes. manipulated holographic tweezers (HOT). succeeded in high-speed using HOT (transport speed:...
We developed Optical pH regulation using functional nanotool impregnated with photo-responsive chemical for selective cell injection of nanotool. The was modified by fluorescent dye intracellular measurement. included in the fusogenic liposome. Membrane fusion liposome to membrane used invasive fuses weak acidic condition. Local inside photochromic Leuco crystal violet (LCV). LCV emits proton ultraviolet (UV) illumination. emitted decreases value This is reversible UV/VIS manipulated optical...
The continual development of surgical technology has led to a demand for simulators evaluating and improving the technique surgeons. To meet these needs, must incorporate sensing function into organ model techniques. However, it is difficult temperature sensor conventional cardiac training model. In this study, we propose heart catheter ablation made from hydrogel, which memory properties. consists photo-crosslinkable hydrogel mixed with an irreversible indicator that exhibits color change...