A5040504587- Reproductive tract infections research
- Virus-based gene therapy research
- Clostridium difficile and Clostridium perfringens research
- Insect and Pesticide Research
- Dermatology and Skin Diseases
- Viral gastroenteritis research and epidemiology
- Animal testing and alternatives
- Cervical Cancer and HPV Research
- Helminth infection and control
- Animal Behavior and Welfare Studies
- Dermatological diseases and infestations
- Gut microbiota and health
- Ion Transport and Channel Regulation
- Diphtheria, Corynebacterium, and Tetanus
- Antifungal resistance and susceptibility
- Complementary and Alternative Medicine Studies
- Insect Utilization and Effects
- Parvovirus B19 Infection Studies
- Spine and Intervertebral Disc Pathology
- Lysosomal Storage Disorders Research
- Veterinary Orthopedics and Neurology
- Radiation Therapy and Dosimetry
- Forensic Toxicology and Drug Analysis
- Viral Infections and Immunology Research
- Parasites and Host Interactions
Weill Cornell Medicine
2017-2025
Memorial Sloan Kettering Cancer Center
2015-2025
Cornell University
2015-2025
Rockefeller University
2010-2025
Tri-Institutional PhD Program in Chemical Biology
2010-2025
Johns Hopkins Medicine
2004
Johns Hopkins University
2003-2004
Alzheimer's disease (AD) is a progressive degenerative neurological disorder affecting nearly one in nine elderly people the United States. Population studies have shown that an inheritance of apolipoprotein E (APOE) variant APOE4 allele increases risk developing AD, whereas APOE2 homozygotes are protected from late-onset AD. It was hypothesized expression "protective" by genetic modification central nervous system (CNS) could reverse or prevent neurologic damage. To assess CNS distribution...
Chlamydia muridarum (Cm), an intracellular bacterium of historical importance, was recently rediscovered as moderately prevalent in research mouse colonies. Cm first reported a causative agent severe pneumonia mice about 80 y ago, and while it has been used experimentally to model trachomatis infection humans, there have no further reports clinical disease associated with natural infection. We observed pathology 2 genetically engi- neered (GEM) strains, Il12rb2 KO STAT1 KO, impaired...
Vascular catheter-related infections, primarily caused by Candida albicans and parapsilosis, pose significant challenges due to the formation of biofilms on catheters, leading refractory disease considerable morbidity. We studied efficacy micafungin in systemic lock therapies eliminate catheter-based deep tissue infections experimental central venous catheter (CVC)-related candidemia neutropenic rabbits. Silastic CVCs rabbits were inoculated with 1 × 103 CFU/mL C. or establishing biofilm,...
Cocaine use disorders are mediated by the cocaine blockade of dopamine transporter in central nervous system (CNS). On basis concept that these effects could be obviated if were prevented from reaching its cognate receptors CNS, we have developed an anticocaine vaccine, dAd5GNE, based on a analog covalently linked to capsid proteins E1−E3− serotype 5 adenovirus. While vaccine effectively blocks systemically administered brain mediating sequestration blood, fact also has significant...
The most common reason that adults in the United States see their physician is lower back or neck pain secondary to degenerative disc disease. To date, approaches treat disease are confined purely mechanical devices designed either eliminate enable flexibility of diseased motion segment. Tissue engineered intervertebral discs (TE-IVDs) have been proposed as an alternative approach and shown promise replacing native IVD rodent tail spine. Here we demonstrate efficacy our TE-IVDs canine...
In the past decade, NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG, NOD gamma) mice have become a model of choice in several areas biomedical research; however, comprehensive data on their spontaneous age-related pathology are not currently available literature. The prevalence morbidity affecting aged NSG female breeders enrolled parasitology study was documented with classification neoplastic and non-neoplastic (inflammatory, metabolic, degenerative) lesions. Malignant mammary neoplasms were...
Corynebacterium bovis (Cb), the etiologic agent of -associated hyperkeratosis (CAH) in nude mice, may impact research outcomes. Little is known about differences course and severity CAH different outbred athymic mice stocks. Three genetic stocks (designated A, B, C), 1 which was obtained from 2 geographically separate colonies with distinct microbiota (A1 A2), were inoculated topically × 10 8 cfu a pathogenic Cb field isolate (no. 7894; n = 6/stock) or sterile media ( 2/stock; controls)....
The soles of staff shoes accessing vivaria can become contaminated on urban streets, potentially serving as a source fomite-mediated transmission adventitious agents to laboratory rodents. While shoe covers may mitigate this risk, donning them lead hand contamination. Staff our use motor-driven cleaners hundreds times daily remove and collect particulates via vacuum collection system from the top, sole, sides instead covers. Shoe cleaner debris (SCD) contact media (CM) exposed SCD in 5 were...
Standard methods for risk assessments resulting from human exposures to mixed radiation fields in Space consisting of different particle types and energies rely upon quality factors. These are generally defined as a function linear energy transfer (LET) assumed be proportional the risk. In this approach, it is further that risks single each add linearly. Although cancer acute photon radiations have been measured humans, factors protons ions heavier atomic mass inferred animal and/or cellular...
Metachromatic leukodystrophy, a fatal pediatric neurodegenerative lysosomal storage disease caused by mutations in the arylsulfatase A (ARSA) gene, is characterized intracellular accumulation of sulfatides lysosomes cells central nervous system (CNS). In previous studies, we have demonstrated efficacy AAVrh.10hARSA, an adeno-associated virus (AAV) serotype rh.10 vector coding for human ARSA gene to CNS mouse model disease, and that catheter-based intraparenchymal administration AAVrh.10hARSA...
Chlamydia muridarum (Cm) was detected in 2 colonies of mice with lymphoplasmacytic pulmonary infiltrates by using PCR and immunohistochemistry. This discovery unexpected, as Cm infection had not been reported laboratory since the 1940s. A specific assay developed testing implemented for resident 8 vivaria from 3 academic institutions, 58 incoming mouse shipments 39 received 55 commercial breeding (4 vendors). To estimate Cm's global prevalence research colonies, a database containing 11,387...
The murine bacterial pathogen
Friedreich's ataxia (FA) is a life-threatening autosomal recessive disorder characterized by neurological and cardiac dysfunction. Arrhythmias heart failure are the main cause of premature death. From prior studies in murine models FA, adeno-associated virus encoding normal human frataxin gene (AAVrh.10hFXN) effectively treated manifestations disease. However, therapeutic dose window limited high level (hFXN) expression associated with toxicity. As goal, since FA heterozygotes have no...
Abstract Chlamydia muridarum (Cm) has reemerged as a prevalent bacterial contaminant of academic research mouse colonies. A study was conducted to assess the effectiveness husbandry and cage sanitization methods in preventing intercage transmission Cm. To during change, housing 2 Cm-free Swiss Webster (SW; Tac:SW) sentinel mice placed randomly on each 12 individually ventilated racks, cages with Cm-shedding mice, located one animal holding rooms. Husbandry staff blinded changed all rooms...
Discectomies are a common surgical treatment for disc herniations in the canine spine. However, effect of these procedures on intervertebral tissue is not fully understood. The objective this study was to assess degenerative changes cervical spinal segments undergoing discectomy procedures, vivo. led 60% drop height and 24% foraminal height. Segments did fuse but showed osteophyte formation as well endplate sclerosis. MR imaging revealed terminal with collapse space loss T2 signal intensity....
(Cm) has reemerged as a prevalent bacterial contaminant of academic research mouse colonies. A study was conducted to assess the effectiveness husbandry and cage sanitization methods in preventing intercage transmission Cm. To during change, housing 2 Cm-free Swiss Webster (Tac:SW; SW) sentinel mice placed randomly on each 12 individually ventilated racks, cages with Cm-shedding mice, located 1 animal holding rooms. Husbandry staff blinded cages, changed all rooms weekly using microisolator...
Based on studies in experimental animals demonstrating that administration of adeno-associated virus (AAV) vectors to the cerebrospinal fluid (CSF) is an effective route transfer genes nervous system, there are increasing number clinical trials using CSF treat system disorders. With knowledge turns over four five times daily, and evidence at least some administered AAV distributed systemic organs, we asked: with CSF, what fraction total dose remains goes off target delivered systemically? To...