Mid-infrared photothermal microscopy is a new chemical imaging technology in which visible beam senses the effect induced by pulsed infrared laser. This provides spectroscopic information at submicrometer spatial resolution and enables spectroscopy of living cells organisms. Yet, current mid-infrared sensitivity suffers from weak dependence scattering on temperature, image quality vulnerable to speckles caused scattering. Here, we present novel version thermosensitive fluorescent probes are...

Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity fingerprint...

Abstract Confronted with the rapid evolution and dissemination of antibiotic resistance, there is an urgent need to develop alternative treatment strategies for drug‐resistant pathogens. Here, unconventional approach presented restore susceptibility methicillin‐resistant S. aureus (MRSA) a broad spectrum conventional antibiotics via photo‐disassembly functional membrane microdomains. The microdomains based on effective photolysis staphyloxanthin, golden carotenoid pigment that gives its...

Label-free stimulated Raman scattering microscopy resolves amphotericin B orientation in fungal cell membrane.

High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological diseases. Current non-invasive methods offer limited at the millimeter level. Here, we report optically-generated focused ultrasound (OFUS) for brain stimulation with ultrahigh precision. OFUS generated by soft optoacoustic pad (SOAP) fabricated through embedding candle soot nanoparticles in curved polydimethylsiloxane film. SOAP generates transcranial focus 15 MHz an lateral resolution of 83...

Bacteria have evolved to cope with the detrimental effects of ROS using their essential molecular components. Catalase, a heme-containing tetramer protein expressed universally in most aerobic bacteria, plays an indispensable role scavenging excess hydrogen peroxide (H2O2). Here, through use wild-type and catalase-deficient mutants, we identified catalase as endogenous therapeutic target 400-420 nm blue light. Catalase residing inside bacteria could be effectively inactivated by light,...

Microbes have developed their own specific strategies to cope with reactive oxygen species (ROS). Catalase, a heme-containing tetramer expressed in broad range of aerobic fungi, shows remarkable efficiency degrading hydrogen peroxide (H

Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally sensitive solid-state optical parametric oscillator (OPO) in current SRS microscope. Moreover, chemically informative multiwindow across C–H, C–D, fingerprint regions challenging due to slow wavelength tuning speed OPO. In this work, we present system based on compact robust fiber laser with rapid wide...

Candida albicans (C. albicans), a major fungal pathogen, causes life-threatening infections in immunocompromised individuals. Fluconazole (FLC) is recommended as first-line therapy for treatment of invasive infections. However, the widespread use FLC has resulted increased antifungal resistance among different strains Candida, especially C. albicans, which leading source hospital-acquired Here, by hyperspectral stimulated Raman scattering imaging single cells fingerprint window and...

Hyphae formation is a key step for fungal penetration into epithelial cells and escaping from macrophages or neutrophils. We found that 405 nm light-induced catalase deactivation results in the inhibition of hyphae growth Candida albicans. The treatment capable inhibiting across multiple hyphae-producing species. Metabolic studies on light-treated C. albicans reveal light strong reduction both lipid protein metabolism. A significant decrease unsaturated saturated fatty acids was detected...

We present a novel version of mid-infrared photothermal microscopy in which thermosensitive fluorescent probes are harnessed to sense the effect. The fluorescence intensity can be modulated at level 1% per Kelvin, is 100 times larger than modulation scattering intensity. In addition, emission free interference, thus much improving image quality. Moreover, fluorophores target specific organelles or biomolecules, augmenting specificity imaging. Spectral fidelity confirmed by fingerprinting...

Abstract Confronted with the rapid evolution and dissemination of antibiotic resistance, there is an urgent need to develop alternative treatment strategies for drug-resistant pathogens. Here, we present unconventional approach restore susceptibility methicillin-resistant S. aureus (MRSA) a broad spectrum conventional antibiotics via photo-disassembly functional membrane microdomains. The microdomains based on effective photolysis staphyloxanthin, golden carotenoid pigment that gives its...

Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally-sensitive solid-state optical parametric oscillator (OPO) in current SRS microscope. Moreover, chemically-informative multi-window across C-H, C-D fingerprint regions challenging due to slow wavelength tuning speed OPO. In this work, we present system based on compact robust fiber laser with rapid widely...

Catalase plays an essential role in degrading hydrogen peroxide (H2O2), which is one of the major enzymatic ROS scavenging mechanisms. Here, using wild-type Candida albicans along with its catalase-deficient mutant, we report that catalase inside fungi could be effectively and universally inactivated by blue light 410 nm, subsequently rendering these pathogens extremely sensitive to H2O2 ROS-generating agents. This strategy also significantly eradicate multiple notorious clinical strains,...

Abstract Bacteria have evolved to cope with the detrimental effects of reactive oxygen species (ROS) using their essential molecular components. Catalase, a heme-containing tetramer protein expressed universally in most aerobic bacteria, plays an indispensable role scavenging excess hydrogen peroxide (H 2 O ). Here, through utilization wild-type and catalase-deficient mutants, we identified catalase as endogenous therapeutic target 400-420 nm blue light. Catalase residing bacteria could be...

The rapid evolution of antibiotic resistance increasingly challenges the successful treatment of<i> S. aureus </i>infections. Here, we present an unconventional approach by disassembly its membrane microdomains via pulsed laser photolysis staphyloxanthin. After staphyloxanthin photolysis, permeabilization, fluidification, and protein detachment, were found underlying mechanisms to malfunction defense several major classes conventional antibiotics. Through selection study, completely depleted...

Candida auris, the deadly infectious fungus, was reported to infest nearly 60 hospitals and more than 90 nursing homes in New York City. Moreover, these fungal species have developed resistance all three major anti-fungal drugs. Drug-resistant spp. other non-albicans multi-drug around world. Here, we show that, through efficient photoinactivation of an essential detoxifying enzyme which exists most strains, could achieve significant eradication those pathogens by subsequent administration...

In article number 1903117, Ji-Xin Cheng and co-workers report an endogenous chromophore-based phototherapy platform to combat antibiotic-resistance Staphylococcus aureus infections. Functional membrane microdomains of are catastrophically disrupted via photochemical decomposition membrane-bound carotenoid pigment, staphyloxanthin, thus reviving a broad spectrum conventional antibiotics against aureus.

Candidemia remains the fourth most common cause of nosocomial bloodstream infections. For more than a half-century, amphotericin B (Amp B) has been last line defense in treatment life-threatening fungal However, during past several years, severe infections due to Amp B-resistant Candida spp. isolates have increasingly reported. Here, through polarization stimulated Raman scattering microscopy, was found accumulate largely cell membrane highly orientated approach, and interaction between...

Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding broad range of applications. Due to the limited spectral coverage current SRS systems, most applications utilized narrow band (<300 cm-1). Multi-window covering C-H, C-D and fingerprint regions offers richer chemical information. In this work, we present multi-window system with rapid widely tunable fiber laser. We implemented auto-balanced detection enhance signal-to-noise ratio loss by 23 times....

Volumetric chemical imaging is highly desired for investigating biochemical processes at the sub-cellular level. Here, we report bond-selective intensity diffraction tomography (BS-IDT) based on 3D quantitative phase detection of mid-infrared photothermal effect. BS-IDT demonstrates volumetric with incoherent diffraction-limited resolution and a high speed up to ~6 Hz per volume. The mid-IR spectrum extracted from shows fidelity compared ground truth measured by an FTIR spectrometer. results...

High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological diseases. Current non-invasive methods offer limited at the millimeter level. Here, we report optically-generated focused ultrasound (OFUS) for brain stimulation with ultrahigh precision. OFUS generated by soft optoacoustic pad (SOAP) fabricated through embedding candle soot nanoparticles in curved polydimethylsiloxane film. SOAP generates transcranial focus 15 MHz an lateral resolution of 83...