A5000487114- biodegradable polymer synthesis and properties
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Microplastics and Plastic Pollution
- Sugarcane Cultivation and Processing
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Transgenic Plants and Applications
- Potato Plant Research
- Botany and Plant Ecology Studies
- Plant tissue culture and regeneration
- Agricultural Productivity and Crop Improvement
- Bioenergy crop production and management
- Plant Pathogens and Resistance
- Electrochemical Analysis and Applications
- CRISPR and Genetic Engineering
- Analytical Chemistry and Sensors
- Conducting polymers and applications
- Electrocatalysts for Energy Conversion
- Biodiesel Production and Applications
- Botanical Studies and Applications
- Research in Cotton Cultivation
Yield10 Bioscience (United States)
2007-2023
Massachusetts Institute of Technology
1996-2001
Michigan State University
1996
University of Miami
1981
University of Cincinnati Medical Center
1977
Landesforschungsanstalt für Landwirtschaft und Fischerei Mecklenburg-Vorpommern
1929-1939
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOverexpression and Purification of the Soluble Polyhydroxyalkanoate Synthase from Alcaligenes eutrophus: Evidence for a Required Posttranslational Modification Catalytic ActivityT. U. Gerngross, K. D. Snell, O. P. Peoples, A. J. Sinskey, E. Csuhai, S. Masamune, StubbeCite this: Biochemistry 1994, 33, 31, 9311–9320Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August...
Polyhydroxyalkanoate bio-based plastics made from renewable resources can reduce petroleum consumption and decrease plastic waste disposal issues as they are inherently biodegradable in soil, compost marine environments. In this paper, the successful engineering of biomass crop switchgrass (Panicum virgatum L.) for synthesis polyhydroxybutyrate (PHB) is reported. Polymer production was monitored more than 400 primary transformants grown under vitro glasshouse conditions. Plants containing up...
Abstract The petroleum industry has optimized profits by producing value‐added coproducts, such as plastics and chemicals, in addition to primary liquid fuels. A similar coproduct strategy applied biorefineries processing cellulosic biomass fuels and/or energy would transform a technology that is marginally economic, depending on oil prices, sustainable business with enhanced revenue streams from multiple coproducts. challenge finding biobased compatible biorefinery scenario where markets...
An optimized genetic construct for plastid transformation of tobacco (Nicotiana tabacum) the production renewable, biodegradable plastic polyhydroxybutyrate (PHB) was designed using an operon extension strategy. Bacterial genes encoding PHB pathway enzymes were selected use in this based on their similarity to codon usage and GC content plastome. Regulatory elements with limited homology host plastome yet known yield high levels plastidial recombinant protein used enhance expression...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTPolyhydroxybutyrate Synthase: Evidence for Covalent CatalysisJ. Wodzinska, K. D. Snell, A. Rhomberg, J. Sinskey, Biemann, and StubbeView Author Information Departments of Chemistry Biology Massachusetts Institute Technology Cambridge, 02139Cite this: Am. Chem. Soc. 1996, 118, 26, 6319–6320Publication Date (Web):July 3, 1996Publication History Received4 April 1996Published online3 July inissue 1 January...
Summary Polyhydroxybutyrate (PHB) is a bacterial polyester that has properties similar to some petrochemically produced plastics. Plant‐based production the potential make this biorenewable plastic highly competitive with petrochemical‐based We previously reported transgenic sugarcane PHB at levels as high 1.8% leaf dry weight without penalty biomass accumulation, suggesting scope for improving in species. In study, we used different plant and viral promoters, combination multigene or...
Direct evidence is presented for the production of an exotoxin by Pseudomonas aeruginosa multiplying at burned site in infected mouse. toxin was assayed measurement its ability to catalyze transfer radioactivity from [14C] adenine-labeled nicotinamide adenine dinucleotide elongation factor 2 (adenosine diphosphate ribosylation activity). This enzyme activity found saline extracts skin but not present similar uninfected skin. It detected serum animals 26 hr after infection. The level active...
Summary Poly‐3‐hydroxybutyrate ( PHB ) production in plastids of C amelina sativa seeds was investigated by comparing levels polymer produced upon transformation plants with five different binary vectors containing combinations seed‐specific promoters for expression transgenes. Genes encoding biosynthetic enzymes were modified at the N ‐terminus to encode a plastid targeting signal. up 15% mature seed weight measured single sacrificed T 1 genetic construct oleosin and glycinin promoters. A...
ABSTRACT Expression of Escherichia coli open reading frame yfcX is shown to be required for medium-chain-length polyhydroxyalkanoate (PHA MCL ) formation from fatty acids in an E. fadB mutant. The encodes a protein, YfcX, with significant similarity the large subunit multifunctional β-oxidation enzymes. strains modified contain inactivated copy and express synthase are unable form PHA s when grown presence acids. Plasmid-based expression FadB − YfcX PhaC + strain restores polymer formation....
The production of polyhydroxybutyrate (PHB) involves a multigene pathway consisting thiolase, reductase and synthase genes. In order to simplify this for plant-based expression, library thiolase gene fusions was generated by randomly ligating short core linker DNA sequence create in-frame between the resulting fusion constructs were screened PHB formation in Escherichia coli. This screen identified polymer-producing candidate which genes fused via 26-amino-acid linker. fusion, designated...
Summary Engineering the production of polyhydroxyalkanoates ( PHA s) into high biomass bioenergy crops has potential to provide a sustainable supply bioplastics and energy from single plant feedstock. One major challenges in engineering C 4 plants for poly[( R )‐3‐hydroxybutyrate] PHB ) is significantly lower level polymer produced chloroplasts mesophyll (M) cells compared bundle sheath BS cells, thereby limiting full yield‐potential plant. In this study, we evidence that access substrate...
The yield of l-phenylalanine, racemic phenyllactic acid, and prephenic acid synthesized from glucose has been doubled contamination these aromatic end products by biosynthetic intermediates drastically reduced. These improvements resulted increasing the in vivo catalytic activity specific enzymes common pathway amino biosynthesis chromosomal modification Escherichia coli. centerpiece changes was synthesis a multigene cassette carrying aroA (encoding EPSP synthase), aroC chorismate aroB DHQ...
Polyhydroxyalkanoates are linear biodegradable polyesters produced by bacteria as a carbon store and used to produce range of bioplastics. Widespread polyhydroxyalkanoate production in C4 crops would decrease petroleum dependency producing renewable supply plastics along with residual biomass that could be converted into biofuels or energy. Increasing yields commercial levels however remains challenge. Previously, lower accumulation the short side chain polyhydroxyalkanoate,...