A5063714502- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Asthma and respiratory diseases
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Electrospun Nanofibers in Biomedical Applications
- Computational Drug Discovery Methods
- Body Contouring and Surgery
- Immune cells in cancer
- Allergic Rhinitis and Sensitization
- Reproductive System and Pregnancy
- Hernia repair and management
- Macrophage Migration Inhibitory Factor
- Psychological Treatments and Disorders
- Immune Cell Function and Interaction
- Medical research and treatments
- Periodontal Regeneration and Treatments
- Congenital heart defects research
- Wound Healing and Treatments
- Plant Reproductive Biology
- Respiratory and Cough-Related Research
- Hypothalamic control of reproductive hormones
- Cell Adhesion Molecules Research
Centro de Cirugía de Mínima Invasión Jesús Usón
2020-2025
Instituto de Salud Carlos III
2025
Spanish Clinical Research Network
2023
Universidad Autónoma de Madrid
2019-2022
Hospital Universitario Fundación Jiménez Díaz
2019-2022
Universidad de Oviedo
2015
Abstract Introduction Myocardial infarction (MI) remains a leading global cause of mortality and is commonly treated with Captopril, Spironolactone, Bisoprolol. Emerging therapies, such as the secretome derived from cardiosphere-derived cells (S-CDCs), have shown potential in reducing MI scar size. This study aims to evaluate therapeutic implications S-CDCs treatment, particularly relation aforementioned standard drugs. Methods CDCs (n = 4) were isolated cardiac explants Large White pigs...
Abstract Introduction Myocardial infarction (MI) initiates an acute inflammatory response that is crucial for heart repair. However, exaggerated can lead to adverse left ventricular remodeling and failure. The secretome of cardiosphere-derived cells (CDCs) offers promising immunomodulatory properties. This study seeks determine the optimal timing CDC application by evaluating CD163 expression in monocytes plasma levels post-MI maximize its Methods was evaluated under basal conditions after...
Abstract Introduction Treatment of ovarian cancer (OC) usually consists a combination surgery and chemotherapy, with carboplatin, often in paclitaxel, being the most employed drug regimen. While effective, this approach has significant side effects is associated long-term chemoresistance, highlighting need for alternative therapeutic strategies. This study aims to evaluate, through silico methods, potential secretome from menstrual blood-derived stromal cells (MenSCs) as adjuvant...
Abstract Introduction The treatment of myocardial infarction (MI) remains a challenge in cardiovascular medicine. While the combined use Captopril, Bisoprolol, and Spironolactone is well-established MI management, there still need to develop new therapeutic approaches. This study evaluates potential secretome derived from menstrual blood stromal cells (MenSCs) through silico analysis, comparing its effects with those aforementioned drugs. Methods MenSCs (n=5) were cultured DMEM + 1% ITS for...
Mesenchymal stromal cells (MSCs) have been shown to exert their therapeutic effects through the secretion of broad spectrum paracrine factors, including extracellular vesicles (EVs). Accordingly, EVs are being pursued as a promising alternative cell-based therapies. Menstrual blood-derived (MenSCs) type MSC that, due immunomodulatory and regenerative properties, emerged an innovative source. Additionally, new strategies cell priming may potentially alter concentration cargo released EVs,...
Mesenchymal stromal cells isolated from menstrual blood (MenSCs) exhibit a potent pro-angiogenic and immunomodulatory capacity. Their therapeutic effect is mediated by paracrine mediators released their secretomes. In this work, we aimed to evaluate the of specific priming condition on phenotype secretome content MenSCs. Our results revealed that optimal for MenSCs was combination interferon gamma (IFNγ) tumor necrosis factor alpha (TNFα) produced synergistic additive IDO1 release...
Experimental data demonstrated that the regenerative potential and immunomodulatory capacity of Cardiosphere-Derived Cells (CDCs) is mediated by paracrine mechanisms. In this process, extracellular vesicles derived from CDCs (EV-CDCs) are key mediators their therapeutic effect. Considering future applicability these in human diseases, an accurate preclinical-to-clinical translation needed, as well exhaustive molecular characterization animal-derived products. Based on that, main goal study...
Asthma is a complex disease comprising various phenotypes and endotypes, all of which still need solid biomarkers for accurate classification. In previous study, we defined specific genes related to asthma respiratory allergy by studying the expression 94 in population composed 4 groups subjects: healthy control, nonallergic asthmatic, asthmatic allergic, nonasthmatic allergic patients. An analysis differential gene between controls patients revealed set statistically relevant mainly...
Macrophage scavenger receptor 1 (MSR1) has mostly been described in macrophages, but we previously found a significant gene expression increase peripheral blood mononuclear cells (PBMCs) of asthmatic patients.To confirm those results and to define its cellular origin PBMCs.Four groups subjects were studied: healthy controls (C), nonallergic (NA), allergic (AA), chronic obstructive pulmonary disease (COPD) patients. RNA was extracted from PBMCs. MSR1 analyzed by RT-qPCR. The presence on the...
Abstract Olive-pollen allergy is one of the leading causes respiratory in Mediterranean countries and some areas North America. Currently, allergen-specific immunotherapy only etiophatogenic treatment. However, this approach not fully optimal, safe, or effective. Thus, efforts continue search for novel strategies, being most promising use peptides derived from major allergens. This work tries to determine therapeutic potential safety 5 dodecapeptides main allergen olive-pollen allergy, Ole e...
Acute myocardial infarction (AMI) is the consequence of an acute interruption blood flow delimiting area with ischemic necrosis. The loss cardiomyocytes initiates cardiac remodeling in myocardium, leading to molecular changes attempt recover function. purpose this study was unravel differences profile between and remote myocardium after AMI experimental model. To mimic human infarction, healthy pigs were subjected occlusion mid-left anterior descending coronary artery, tissue collected from...
Abstract Background Despite constant advances in regenerative medicine, the closure of chronic wounds is still challenging. Therapeutic approaches using locally administered MSCs have been considered a promising option. However, viability these cells seriously threatened by acute hypoxic stress linked to wound healing. In this work, we aimed study tolerance Menstrual blood-derived stromal (MenSCs) hypoxia and their therapeutic paracrine effect. Methods Isolated MenSCs were phenotypically...
Highly prevalent respiratory diseases such as asthma and allergy remain a pressing health challenge. Currently, there is an unmet need for precise diagnostic tools capable of predicting the great heterogeneity these illnesses. In previous study 94 asthma/respiratory biomarker candidates, we defined group potential biomarkers to distinguish clinical phenotypes (i.e. nonallergic asthma, allergic without asthma) disease severity. Here, analyze our experimental results using complex algorithmic...
Polypropylene (PP) mesh is well-known as a gold standard of all prosthetic materials choice for the reinforcement soft tissues in case hernia, organ prolapse, and urinary incontinence. The adverse effects that follow surgical implantation remain an unmet medical challenge. Herein, it outlined new approach to allow viability adhesion human menstrual blood-derived mesenchymal stromal cells (MenSCs) on PP meshes. A multilayered fibrin coating, based fibrinogen thrombin from commercial sealant,...
Acute myocardial infarction (AMI) is a manifestation of ischemic heart disease where the immune system plays an important role in re-establishment homeostasis. We hypothesize that anti-inflammatory activity secretomes from menstrual blood-derived mesenchymal stromal cells (S-MenSCs) and IFNγ/TNFα-primed MenSCs (S-MenSCs*) may be considered therapeutic option for treatment AMI. To assess this hypothesis, we have evaluated effect S-MenSCs S-MenSCs* on cardiac function parameters involvement...
Abstract Endometrial Mesenchymal Stromal Cells (endMSCs) can be easily isolated from menstrual blood by plastic adherence. These cells have a potent pro-angiogenic and immunomodulatory capacity, their therapeutic effect is mediated paracrine mechanisms where secretome key role. In this paper, we aimed to evaluate different priming conditions in endMSCs using pro-inflammatory cytokines Toll-Like Receptor ligands. Our vitro results revealed synergistic additive of IFNγ TNFα on endMSCs. The...