Human amniotic epithelial cells (hAECs), having the characteristics of both embryonic and pluripotent stem cells, have potential to differentiate into various cells. A good deal research has explored clinical therapeutic hAECs; rat been reported ameliorate functional deficits after stroke in rats, likely due neuronal differentiation cytokine secretion by these We isolated hAECs transfected them with glial cell line-derived neurotrophic factor (GDNF) or enhanced green fluorescent protein...

The development of a technique to induce the transformation somatic cells pluripotent state via ectopic expression defined transcription factors was transformational event in field regenerative medicine. this also impacted ophthalmology, as patient-specific induced stemcells (iPSCs) may be useful resources for some ophthalmological diseases. lens is key refractive element eye that focuses images visual world onto retina. To establish new model drug screening treat diseases and investigating...

Pancreatic islet transplantation has demonstrated that long-term insulin independence may be achieved in patients suffering from diabetes mellitus type 1. However, limited availability of tissue means new sources insulin-producing cells are responsive to glucose required. Here, we show human amnion epithelial (HAEC) can induced differentiate into functional vitro. After induction differentiation, HAEC expressed multiple pancreatic beta-cell genes, including insulin, pancreas duodenum...

Cancer stem cells (CSCs) play an important role in drug resistance of tumor and are responsible for high recurrence rates. Agents that can suppress the proliferation differentiation CSCs would provide new opportunity to fight against recurrence. In this study, we developed a strategy enrich human osteosarcoma cell line hMG63. Using these as model, tested effect bufalin, traditional Chinese medicine, on CSCs. hMG63 were cultured poly-HEMA-treated dish cancer cell-specific medium. nonadhesive...

Recent progress in regenerative medicine has suggested that mesenchymal stem cell (MSC)-based therapy is a novel potential cure for diabetes. Betatrophin newly identified hormone can increase the production and expansion of insulin-secreting β-cells when administered to mice. In this study, we evaluated effect betatrophin overexpression by human adipose-derived MSCs (ADMSCs) vitro experiments, as well following their transplantation into mice with streptozotocin (STZ)-induced The did not...

Pancreatic islet transplantation has demonstrated that long-term insulin independence may be achieved in patients suffering from diabetes mellitus type 1. However, limited availability of tissue means new sources insulin-producing cells are responsive to glucose required. Here, we show human amnion epithelial (HAEC) can induced differentiate into functional vitro. After induction differentiation, HAEC expressed multiple pancreatic β-cell genes, including insulin, pancreas duodenum...

Cataractogenesis begins from the dynamic lens epithelial cells (LECs) and adjacent fiber cells. LECs derived cell lines cannot maintain crystalline expression as primary LECs. The current study aimed to efficiently generate large numbers of human patient-specific induced pluripotent stem (iPSCs). Anterior capsules were collected cataract surgery used culture hLECs. iPSCs these hLECs by lentiviral transduction Oct4, Sox2, Klf4, c-Myc. Then, generated re-differentiated into 3-step addition...

Mouse and human embryonic stem (ES) cells are usually maintained in an undifferentiated state by coculture with mouse fibroblasts (MEFs) as feeder cells. In the case of ES cells, addition leukemia inhibitory factor (LIF) to culture media is also necessary prevent cell differentiation. Here, we report use primary amnion epithelial (hAECs) using our newly developed protocols. We found that grown on hAECs express markers including FGF, Oct-4, Nanog, Sox-2, Rex, TERT. Interestingly, expression...

Mouse embryonic stem cells (ESCs) are typically cultured on a feeder layer of mouse fibroblasts (MEFs), with leukemia inhibitory factor (LIF) added to maintain them in an undifferentiated state. We have previously shown that human amniotic epithelial (hAECs) can be used as ESC pluripotency, but the mechanism for this is unknown. In present study, we found CpG islands 5' c-Myc gene remain hypomethylated ESCs hAECs. addition, levels acetylation histone H3 and trimethylation H3K4 promoter were...

Traditionally, fully polarimetric calibration of a nonreciprocal radar system requires measurements at least two passive calibrators, such as dihedral corner reflector plus metal plate or sphere. Interchanging multiple calibrators results in not only higher complexity but also degraded uncertainty. In this work, new calibrator is proposed, which designed quarter concave cylinder linked (QCCLD). The backscattering single QCCLD contains both depolarizing and nondepolarizing components when...

Currently, a set of scaled squat circular cylinders (SCCs) have been widely used for co-polarimetric radar cross section (RCS) calibration in various test ranges where low RCS metal pylons are used. The major drawback cylinder (CC) is its high sensitivity to elevation angle, as well the frequency-dispersive theoretical RCS. In this work, we propose new calibrator, ellipsoidal (EC), whose characteristics similar sphere, while it can be mounted on pylon with little electromagnetic (EM)...

Human embryonic stem cells (hESCs) are usually maintained in an undifferentiated state by coculture with mitomycin C-treated mouse fibroblasts (MEFs) as feeder the presence of animal sera such fetal bovine serum (FBS). Here, we use primary human amnion epithelial (hAECs) and umbilical cord blood (CBS) a replacement for FBS to support growth hESCs. The 5∼10-fold higher expression levels ES cell markers including FGF, Oct-4, Nanog, Sox-2, Rex, TERT were found hESCs grown on hAECs compared that...

In this paper, a polarimetric Multiple Input Output (MIMO) radar with linear antenna array including 8 transmitters and 12 receivers is developed calibrated for near-field imaging measurement in frequency band spanning from 26.5GHz to 38GHz. the system hardware configuration, single-polarized antennas are connected vector network analyzer (VNA) through RF switch matrix as compromise of performance expense. A two-step calibration procedure proposed compensation signal distortion, where...

Fully polarimetric multiple input output (MIMO) radars are getting more and applications for near-field scattering matrix (PSM) imagery. A MIMO radar consists of a large number transmitting receiving channels. The crosstalk imbalance among the channels severely distort measured PSM. In this paper, fully calibration technique imaging is proposed using single passive calibrator named quarter concave cylinder linked dihedral (QCCLD) reflector which was presented in our previous work [20]....

Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression neuronal phenotypes (microtubule-associated protein-2, glial fibrillary acidic protein and nestin) in human cells. The conditioned medium promoted growth proliferation rat cultured vitro, this effect was dose-dependent. further transplanted into corpus striatum healthy adult rats grafted could integrate with host migrate 1-2 mm along nerve fibers...

Retinal microglia play an important role as resident immunocompetent and phagocytic cells in the event of injury disease. precursor transplantation show a rescue effect ischemic retina retinal degeneration. However, studies have been hampered by difficulty obtaining sufficient numbers microglia. One way to circumvent this is establish permanent cell lines. In present study, we report generation immortalized microglia, T‐MG cells, from postnatal day 3 rat tissue using lentiviral vector...