A5100752341- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Gut microbiota and health
- Renal and related cancers
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Mesenchymal stem cell research
- Endometriosis Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- Immune cells in cancer
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Retinal Diseases and Treatments
- Wnt/β-catenin signaling in development and cancer
- RNA Research and Splicing
Shanghai University of Traditional Chinese Medicine
2016-2025
Yibin University
2023-2024
Jilin University
2022-2024
Zhongshan Hospital
2021-2024
Fudan University
2020-2024
Union Hospital
2022-2024
Sun Yat-sen University
2020-2023
The First Affiliated Hospital, Sun Yat-sen University
2022-2023
Huazhong Agricultural University
2020-2022
Yale University
2017-2022
In ovarian cancer, CD44 + /CD117 stem cells, also known as cancer‐initiating cells (CICs), are highly proliferative, have a low degree of differentiation, and resistant to chemotherapeutics. Therefore, the subpopulation is thought be an important target for novel therapeutic strategies. this study, we investigated role microRNA‐199a (miR‐199a) in cancer cells. Luciferase reporter gene assays confirmed that miR‐199a targets via miR‐199a‐binding site 3′‐UTR. CICs were enriched from human...
Renal cell carcinoma (RCC) is the most common type of primary renal parenchymal malignancy in adults, with a high degree and poor prognosis.Human cancer stem cells (HuRCSCs) are reported to be main cause drug resistance, metastasis, recurrence, prognosis.Erianin low molecular-weight bibenzyl natural product extracted from Dendrobium chrysotoxum, which inhibits vitro vivo activity variety cells.However, molecular mechanisms Erianin's therapeutic effect on HuRCSCs unknown.Here, we isolated...
Background and Aims: Circulating tumor cells (CTCs) are precursors of cancer metastasis. However, how CTCs evade immunosurveillance during hematogenous dissemination remains unclear. Approach Results: We identified CTC-platelet adhesions by single-cell RNA sequencing multiplex immunofluorescence blood samples from multiple types. Clinically, aggregates were associated with significantly shorter progression-free survival overall in patients HCC. In vitro, ex vivo, vivo assays demonstrated...
Abstract Background Our previous studies have identified that miR-125b was overexpressed in type II endometrial carcinoma (EC) cells compared with I using microRNAs microarray. Although recent shown the important role of several tumors and overexpression advanced EC, its function this disease has not yet been defined. In present study, we tried to confirm result microarray further investigated functions find new downstream targets miR-125b. Methods Differential expression detected between EC...
One emerging model for the development of drug-resistant tumors utilizes a pool self-renewing malignant progenitors known as cancer stem cells (CSCs) or cancer-initiating (CICs). The purpose this study was to propagate such CICs from ovarian cell line SKOV3. SKOV3 sphere were selected using 40.0 micromol/l cisplatin and 10.0 paclitaxel in serum-free culture system supplemented with epidermal growth factor, basic fibroblast leukemia inhibitory insulin standard serum-containing system. These...
microRNAs (miRNAs) play a crucial role in tissue development and the pathology of many diseases, however, effects roles miRNAs semen abnormalities infertile males have not yet been investigated. In this study, we analyzed compared miRNA expression profiles abnormal from 86 with normal healthy using an microarray. total, 52 were differentially expressed between males. The differential selected was validated by real time qRT-PCR northern blotting: miR-574-5p, miR-297, miR-122, miR-1275,...
The morbidity and mortality rates associated with non-small-cell lung carcinoma (NSCLC) are increasing every year, placing new demands on existing therapies drugs. Ammonium ferric citrate (AFC) is often used as a food additive for iron supplementation; however, to our knowledge, no studies have investigated whether AFC can induce ferroptosis in NSCLC. In this study, we demonstrated that specific concentrations of effectively inhibit the proliferation invasion cancer cell lines vitro using...
Abstract Background Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder with various manifestations complex etiology. Follicular fluid (FF) serves as the microenvironment for follicular development. However, correlation between concentration of steroid in FF pathogenesis PCOS still unclear. Methods Twenty levels from ten patients women male-factor infertility undergoing vitro fertilization were tested by liquid chromatography-tandem mass spectrometry (LC-MS/MS) order to...
Human amniotic fluid stem cell-derived exosome (HuAFSC-exosome) transplantation is considered a promising treatment for premature ovarian failure (POF). However, its mechanism remains unclear. In this study, exosomes were isolated and enriched from HuAFSC subsets of CD44+/CD105+, the transplanted into POF model in vitro vivo. Our results confirmed that produced by CD44+/CD105+ HuAFSCs could achieve therapeutic effects mouse model. research also showed HuAFSC-exosomes carrying miR-369-3p...
Subretinal fibrosis is a major cause of the poor visual prognosis for patients with neovascular age-related macular degeneration (nAMD). Myofibroblasts originated from retinal pigment epithelial (RPE) cells through epithelial-mesenchymal transition (EMT) contribute to formation. N6-Methyladenosine (m6A) modification has been implicated in EMT process and multiple fibrotic diseases. The role m6A EMT-related subretinal not yet elucidated. In this study, we found that during mouse model...
Context: As a flavonoid compound, kaempferol has great potential in anti-lung cancer therapy, but the mechanism of its therapeutic effect needs further exploration.Objective: To explore on lung cancer, as well capability to regulate gut microbiota and stimulate immune function.Materials & methods: Twenty-four BALB/c mice were divided into four groups.The first two groups, consisting 12 normal mice, administered either PBS or Kaempferol (Kaem) via gavage.The remaining which subcutaneously...
Although many techniques can be used to generate multitype-induced pluripotent stem (iPS) cells from multitype seed cells, improving the efficiency and shortening period of cell reprogramming remain troublesome issues. In this study, iPS CD34⁺ isolated human amniotic fluid (HuAFCs) by flow cytometry, were infected with retroviruses carrying only one factor (Oct4) cultured on epithelial (HuAEC) feeder layers. Approximately 4 5 days after viral infection, some embryonic (ESC)-like colonies...
Aim: We aimed to identify novel exosomal circular RNAs for hepatocellular carcinoma (HCC) diagnosis. Materials & methods: Exosomes were extracted and characterized. The expression level of circRNAs verified via quantitative real-time PCR. diagnostic value candidate was evaluated according the receiver operating characteristic curve analysis. Results: circ_0070396 significantly elevated in HCC patients than other control groups it performed better distinguishing from healthy donors that...
More and more reports have pointed out that rotenone, as an insecticide, has high neurotoxicity reproductive toxicity to livestock mammals. As a highly physiological correlation system of internal organs, quasi-organs great potential in the fields drug efficacy test, toxicology research, developmental biology so on. In this study, brain organs (mBOs) derived from mouse neural stem cells were used investigate effects rotenone on activity epigenetic modification mBOs. At same time, Rotenone...
Mesenchymal stem cell exosome (MSCs-exo) is a class of products secreted by mesenchymal cells (MSCs) that contain various biologically active substances. MSCs-exo promising alternative to MSCs due their lower immunogenicity and lack ethical constraints. Ginsenoside Rh2 (Rh2) hydrolyzed component the primary substance ginsenosides. has variety pharmacological functions, including anti-inflammatory, anti-tumor, antioxidant. Studies have demonstrated gut microbiota metabolites are critical in...
The Polycomb group protein, enhancer of zeste homolog 2 (EZH2), plays an important role in transcriptional regulation through chromatin remodeling and interactions with other transcription factors to control cell proliferation embryonic development. Previous study has shown that EZH2 is for cycle highly expressed human ovarian cancer. Loss inhibits growth cancer as well carcinomas. In this study, expression plasmid EZH2-targeted microRNA-98 was constructed transfected into stem cells...
// Xiaoyu Cai 1,* , Yuanyuan Hu Hao Tang 2 Han 1 Lijun Pang Junyue Xing Zhenyun Liu Yuhong Luo Bin Jiang Te 4 Myriam Gorospe 3 Chuan Chen and Wengong Wang Department of Biochemistry Molecular Biology, Peking University Health Science Center, Beijing, P. R. China Physiology Pathophysiology, P.R. Laboratory Genetics, National Institute on Aging, Institutes Health, Baltimore, MD, USA Shanghai Geriatric Chinese Medicine, Shanghai, * These authors have contributted equally to this work...
Public–private partnerships have been widely applied in e-government development, contributing to new technologies' adoption and performance. PPP has also required driven related managerial organisational reforms. Progress practice, however, yet be matched by increased research evidence various gaps remain. This study identifies examines four stages of development China the role – outsourcing, service co-delivery, joint management, collaborative governance which are linked developments...
Abstract Objective Thymopentin (5TP) significantly improved typical murine premature ovarian failure (POF) symptoms induced by a high‐fat and high‐sugar (HFHS) diet. However, its effect mechanism remain unclear. Materials methods RNA‐Seq was used to detect the differentially expressed genes among each group. HFHS‐induced POF mouse model generated injected with siRNA using Poly (lactic‐co‐glycolic acid) (PLGA) as carrier. Results suggested that 5TP promoted expression of Yin Yang 2 (YY2) in...
Premature ovarian failure (POF) is the leading cause of female infertility, and there no optimal treatment or medication available currently. For POF, electroacupuncture (EA) has been considered a promising therapeutic approach, but mechanism for this not clear. In study, we explored effects EA (CV4, ST36, SP6) on oxidative stress intestinal microbiota high-fat high-sugar- (HFHS-) induced POF mice. The development mice follicles was observed by hematoxylin eosin (HE) staining. serum levels...