A5073303207- Retinal Development and Disorders
- Retinal Diseases and Treatments
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Plant and fungal interactions
- RNA modifications and cancer
- Marine animal studies overview
- Mycorrhizal Fungi and Plant Interactions
- Ocular Oncology and Treatments
- Gut microbiota and health
- Circular RNAs in diseases
- Plant Pathogens and Fungal Diseases
- Epigenetics and DNA Methylation
- Toxoplasma gondii Research Studies
- Microbial infections and disease research
- Ocular Diseases and Behçet’s Syndrome
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Parasitic Infections and Diagnostics
- Pluripotent Stem Cells Research
- Radiomics and Machine Learning in Medical Imaging
- Neurobiology and Insect Physiology Research
- Barrier Structure and Function Studies
Shanghai First People's Hospital
2019-2025
Shanghai Jiao Tong University
2019-2025
Chinese Academy of Sciences
2013-2025
State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering
2025
Institute of Hydrobiology
2015-2025
Wuhan Polytechnic University
2025
Shanghai Eye Disease Prevention & Treatment Center
2023
Shanghai Clinical Research Center
2023
Yangtze University
2023
National Clinical Research Center for Digestive Diseases
2023
TLR7/9 signals are capable of mounting massive interferon (IFN) response in plasmacytoid dendritic cells (pDCs) immediately after viral infection, yet the involvement epigenetic regulation this process has not been documented. Here, we report that zinc finger CXXC family regulator CXXC5 is highly expressed pDCs, where it plays a crucial role TLR7/9- and virus-induced IFN response. Notably, genetic ablation resulted aberrant methylation CpG-containing island (CGI) within Irf7 gene impaired...
Antiangiogenesis therapies targeting vascular endothelial growth factor (VEGF) have revolutionized the treatment of neovascular ocular diseases, including age-related macular degeneration (nAMD). Compelling evidence has implicated vital role complement system dysregulation in AMD pathogenesis, implying it as a potential therapeutic strategy for geographic atrophy dry and to enhance efficacy anti-VEGF monotherapies nAMD. This study reports preclinical assessment phase 1 clinical outcomes...
Long noncoding RNAs (lncRNAs) or microRNAs belong to the two most important and they are involved in a lot of cancers, including non-small-cell lung cancer (NSCLC). Therefore, currently, we focused on biological clinical significance lncRNA nuclear enriched abundant transcript 1 (NEAT1) hsa-mir-98-5p NSCLC. It was observed that NEAT1 upregulated while downregulated respectively NSCLC cell lines compared human normal epithelial BES-2B cells. Inhibition can suppress progression cells reverse...
In age-related macular degeneration (AMD), one of the principal sources vascular endothelial growth factor (VEGF) is retinal pigment epithelium (RPE) cells under hypoxia or oxidative stress. Solute carrier family 7 member 11 (SLC7A11), a key component cystine/glutamate transporter, regulates level cellular lipid peroxidation, and restrains ferroptosis. our study, we assessed role SLC7A11 in laser-induced choroidal neovascularization (CNV) explored underlying mechanism. We established mouse...
Subretinal fibrosis is a major cause of the poor visual prognosis for patients with neovascular age-related macular degeneration (nAMD). Myofibroblasts originated from retinal pigment epithelial (RPE) cells through epithelial-mesenchymal transition (EMT) contribute to formation. N6-Methyladenosine (m6A) modification has been implicated in EMT process and multiple fibrotic diseases. The role m6A EMT-related subretinal not yet elucidated. In this study, we found that during mouse model...
Base editing technology is an ideal solution for treating pathogenic single-nucleotide variations (SNVs). No gene therapy has yet been approved eye diseases, such as retinitis pigmentosa (RP). Here, we show, in the rd10 mouse model, which carries SNV identified RP-causing mutation human patients, that subretinal delivery of optimized dual adeno-associated virus system containing adenine base editor corrects neuroretina with up to 49% efficiency. Light microscopy showed a thick and robust...
Trithorax group proteins methylate lysine 4 of histone 3 (H3K4) at active gene promoters. MLL5 protein, a member the protein family, has been implicated in control cell cycle progression; however, underlying molecular mechanism(s) have not fully determined. In this study, we found that can associate with regulator “host factor” (HCF-1). The interaction between and HCF-1 is mediated by “HCF-1 binding motif” (HBM) Kelch domain protein. Confocal microscopy showed largely colocalized nucleus....
Mixed lineage leukemia 5 (MLL5) protein is a trithorax family histone 3 lysine 4 (H3K4) methyltransferase that regulates diverse biological processes, including cell cycle progression, hematopoiesis and cancer. The mechanisms by which MLL5 stability regulated have remained unclear to date. Here, we showed cooperatively O-GlcNAc transferase (OGT) ubiquitin-specific protease 7 (USP7). Depletion of OGT in cells led decrease the level through ubiquitin/proteasome-dependent proteolytic...
Trithorax group protein MLL5 is an important epigenetic modifier that controls cell cycle progression, chromatin architecture maintenance, and hematopoiesis. However, whether has a role in innate antiviral immunity largely unknown. Here we show suppresses the RIG-I-mediated anti-viral immune response. Mll5-deficient mice infected with vesicular stomatitis virus enhanced immunity, reduced morbidity, viral load. Mechanistically, fraction of located cytoplasm interacts both RIG-I its E3...
Neovascular age-related macular degeneration (nAMD), characterized by choroidal neovascularization (CNV), is one of the leading causes severe visual impairment and irreversible vision loss around world. Subretinal fibrosis (SRF) contributes to incomplete response anti-vascular endothelial growth factor (VEGF) treatment main reasons for long-term poor outcomes in nAMD. Reducing SRF urgently needed anti-VEGF era. The role non-coding RNAs has been implicated CNV; however, their roles have not...
The orangutan ( Pongo spp.) is a critically endangered species. Today, populations of these animals are rapidly declining by up to 75%. They found in the tropical rainforests Borneo and Sumatra. In this study, using next generation sequencing approach, bacterial fungal diversity fecal material orangutans living Racine Zoo were investigated. most predominant phyla Bacillota along with Bacteroidota. phylum was Ascomycota. Finally, various functions communities present predicted PICRUSt2 KEGG database.
Adenosine triphosphate (ATP)-dependent chromatin remodeling SWI/SNF-like BAF and PBAF complexes have been implicated in the regulation of stem cell function cancers. Several subunits or PBAF, including BRG1, BAF53a, BAF45a, BAF180, BAF250a, are known to be involved hematopoiesis. Baf200, a subunit complex, plays pivotal role heart morphogenesis coronary artery angiogenesis. However, little is on importance Baf200 normal malignant Utilizing Tie2-Cre-, Vav-iCre-, Mx1-Cre-mediated gene deletion...
SYVN1, a gene involved in endoplasmic reticulum-associated degradation, has been found to exert protective effect by inhibiting inflammation retinopathy. This study aimed clarify whether SYVN1 is the pathogenesis of retinopathy prematurity (ROP) and its potential as candidate for target therapy.Human retinal microvascular endothelial cells (hRMECs) mouse model oxygen-induced (OIR) were used reveal development-associated protein expression molecular mechanism. An adenovirus overexpressing or...
Abstract Amyloid β (Aβ) is a crucial component of drusen, the hallmark early stage age‐related macular degeneration (AMD), and can cause retinal pigment epithelium (RPE) cell damage through activation inflammatory response. MicroRNAs play critical role in inflammation. However, mechanism underlying effect microRNAs on NLRP3 inflammasome induced by Aβ remains poorly understood. In present study, we demonstrated that 1‐40 ‐mediated RPE inducing decrease endogenous miR‐191‐5p expression. This...
Abstract Enhanced understanding of normal and malignant hematopoiesis pathways should facilitate the development effective clinical treatment strategies for hematopoietic malignancies. Nuclear receptor corepressor 1 (NCoR1) has been implicated in transcriptional repression embryonic organ development, but its role is yet to be fully elucidated. Here, we showed that hematopoietic-specific loss NCoR1 leads expansion stem cell (HSC) pool due aberrant cycle entry long-term HSCs under...
Abstract Introduction Optic nerve injury is a leading cause of irreversible blindness worldwide. The retinal ganglion cells (RGCs) and their axons cannot be regenerated once damaged. Therefore, reducing RGC damage crucial to prevent blindness. Accordingly, we aimed investigate the potential influence gut microbiota on survival, as well associated action mechanisms. Methods We evaluated effects microbiota, specifically Bifidobacterium , RGC. crush (ONC) was used model optic injury. Vancomycin...
Disordered immune responses and cholesterol metabolism have been implicated in age-related macular degeneration (AMD), the leading cause of blindness elderly individuals. SULT2B1, key enzyme sterol sulfonation, plays important roles inflammation metabolism. However, role underlying mechanism SULT2B1 AMD not investigated thus far. Here, we report that is specifically expressed macrophages choroidal neovascularization lesions. Sutl2b1 deficiency significantly reduced leakage areas inhibited...