A5100422302- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Estrogen and related hormone effects
- Cancer-related gene regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Hormonal Regulation and Hypertension
- Genomics and Chromatin Dynamics
- Cancer Mechanisms and Therapy
- Photochemistry and Electron Transfer Studies
- Cancer Research and Treatments
- Genetic Syndromes and Imprinting
- Bladder and Urothelial Cancer Treatments
- Photochromic and Fluorescence Chemistry
- Genetics and Neurodevelopmental Disorders
- earthquake and tectonic studies
- Reproductive Biology and Fertility
- Telomeres, Telomerase, and Senescence
- Acute Myeloid Leukemia Research
- ATP Synthase and ATPases Research
- Luminescence and Fluorescent Materials
- Histone Deacetylase Inhibitors Research
- Radical Photochemical Reactions
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Shandong Provincial Hospital
2023-2025
Shandong University
2025
Agricultural Genomics Institute at Shenzhen
2024
Chinese Academy of Agricultural Sciences
2024
Zhengzhou University
2024
First Affiliated Hospital of Fujian Medical University
2024
Fujian Medical University
2024
Shandong First Medical University
2023
Yangzhou University
2021-2022
Affiliated Hospital of Southwest Medical University
2022
The glucocorticoid receptor (GR) is phosphorylated at multiple serine residues in a hormone-dependent manner, yet progress on elucidating the function of GR phosphorylation has been hindered by lack simple assay to detect vivo. We have produced antibodies that specifically recognize sites within human Ser(203) and Ser(211). In absence hormone, level Ser(211) was low compared with Ser(203). Phosphorylation both increased upon treatment agonist dexamethasone. Using battery agonists...
The glucocorticoid receptor (GR) is phosphorylated at multiple sites within its N terminus (S203, S211, S226), yet the role of phosphorylation in function not understood. Using a range agonists and GR site-specific antibodies, we demonstrated that transcriptional activation greatest when relative S211 exceeds S226. Consistent with this finding, replacement S226 an alanine enhances response. battery compounds perturb different signaling pathways, found BAPTA-AM, chelator intracellular...
Gliomas with mutant p53 occurring in 30% of glioma patients exhibit therapeutic resistance and poor outcomes. In this study, we identify a novel mechanism through which drives cancer cell survival malignant growth. We documented overexpression the zinc finger protein ZDHHC5 compared normal brain tissue that event tightly correlated mutations. Mechanistic investigations revealed transcriptionally upregulated along nuclear transcription factor NF-Y. These events contributed to development by...
The glucocorticoid receptor (GR) is phosphorylated at three major sites on its N terminus (S203, S211, and S226), phosphorylation modulates GR-regulatory functions in vivo. We examined the site interdependence, contribution of C-terminal ligand-binding domain, participation protein phosphatases GR N-terminal gene expression. found that S203 was greater when S226 not vice versa, indicative intersite dependency. also observed a derivative lacking which no longer binds heat shock 90 (Hsp90)...
Trithorax group proteins methylate lysine 4 of histone 3 (H3K4) at active gene promoters. MLL5 protein, a member the protein family, has been implicated in control cell cycle progression; however, underlying molecular mechanism(s) have not fully determined. In this study, we found that can associate with regulator “host factor” (HCF-1). The interaction between and HCF-1 is mediated by “HCF-1 binding motif” (HBM) Kelch domain protein. Confocal microscopy showed largely colocalized nucleus....
Cellular senescence plays a crucial role in tumorigenesis, development and immune modulation cancers. However, to date, robust reliable cellular senescence-related signature its value clinical outcomes immunotherapy response remain unexplored lung adenocarcinoma (LUAD) patients. Through exploring the expression profiles of 278 genes 936 LUAD patients, (SRS) was constructed validated as an independent prognostic predictor for Notably, patients with high SRS scores exhibited upregulation...
ABSTRACT Unlike other members of the polycomb group protein family, EZH1 has been shown to positively associate with active transcription on a genome-wide scale. However, underlying mechanism for this behavior still remains elusive. Here, we report that physically interacts UXT, small chaperon-like co-activator. UXT specifically and SUZ12, but not EED. Similar upon knockdown or SUZ12 through RNA interference in cell impairs transcriptional activation nuclear factor (NF)-κB target genes...
Abstract The UHRF1-DNMT1 axis plays a key role in DNA maintenance methylation mammals. Accumulative studies demonstrate that UHRF1 is broadly overexpressed cancers, which contributes to cancer cell proliferation and tumorigenesis. Interestingly, proteasome-dependent downregulation of has been observed pluripotent ground state mouse embryonic stem cells (mESCs) cultured the presence two kinase (MEK1/MEK2 GSK3β) inhibitors (termed 2i), raising question whether similarly regulated cells. Here...
Ubiquitin‐specific protease 22 ( USP 22) is a member of the “death‐from‐cancer” signature, which plays key role in cancer progression. Previous evidence has shown that overexpressed and correlates with poor prognosis glioma. The effect mechanism glioma malignancy, especially stemness, remain elusive. Herein, we find more enriched stem‐like tumorspheres than differentiated cells. knockdown inhibits stemness cell lines. With cell‐penetrating TAT ‐tag protein, B cell‐specific Moloney murine...
Understanding the sex determination mechanisms in birds has great significance for biological sciences and production poultry industry. Sex chickens is a complex process that involves fate decisions of supporting cells such as granulosa or Sertoli cells. However, systematic understanding genetic regulation cell commitment underlying still lacking. We aimed to dissect molecular characteristics associated with gonads chicken embryos. Single-nucleus RNA-seq (snRNA-seq) ATAC-seq (snATAC-seq)...
Abstract Background The mammalian liver executes its vital functions through intricate hepatic biochemistry. However, the complexity of metabolome and dynamic alterations during metabolic dysfunction-associated steatotic disease (MASLD) remain poorly understood. Methods We established progressive MASLD mouse models high-fat diet (HFD) high-fat/high-cholesterol (HFHC) dietary-feeding across multiple time points. Utilizing liquid chromatography-mass spectrometry (LC-MS)-based metabolomics...
The human placenta is a glucocorticoid (GC)-responsive organ consisting of multiple cell types including smooth muscle cells, fibroblasts, and trophoblast that demonstrate changes in gene expression after hormone treatment. However, little known about the relative or activity GC receptor (GR) among various placental types. Normal term placentas were examined by immunohistochemistry using either GR phosphorylation site-specific antibodies are markers for activation states antibody recognizes...
Abstract Basinward propagation of fold and thrust belts is a crucial geological process accommodating Cenozoic crustal shortening within the India‐Eurasia collision zone. Anticlinal growth strata in southern Chaiwopu Basin (a piggyback basin) northern Chinese Tian Shan foreland record basinward encroachment along Junggar Frontal Thrust Fault. A new magnetostratigraphic section constrains onset syntectonic at circa 6.4 Ma suggests synchronous thrusting Shan. The intense alluviation ceased...
The methylcytosine dioxygenases TET proteins (TET1, TET2, and TET3) play important regulatory roles in neural function. In this study, we investigated the role of neuronal differentiation using Neuro2a cells as a model. We observed that knockdown TET1, TET2 or TET3 promoted cells, their overexpression inhibited VPA (valproic acid)-induced differentiation, suggesting all three negatively regulate cells. Interestingly, inducing activity protein is independent its enzymatic activity. Our...
Estrogen receptor alpha (ER) coordinates gene expression with cellular physiology in part by controlling receptor- cofactor interactions response to extracellular signals. We have previously shown that the Rho signaling pathway modulates ER transcriptional activation. now demonstrate GDI-dependent increase transactivation is dependent on AF-2 coactivator binding site, prompting us examine regulation of coactivators GDI. Indeed, GDI cooperates GRIP1 ligand-independent and ligand-dependent...