A5100457011- Immune Cell Function and Interaction
- Aquaculture disease management and microbiota
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- T-cell and B-cell Immunology
- Ubiquitin and proteasome pathways
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Aquaculture Nutrition and Growth
- interferon and immune responses
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Astrophysics and Cosmic Phenomena
- Protein Degradation and Inhibitors
- Biosensors and Analytical Detection
- Crustacean biology and ecology
- Particle physics theoretical and experimental studies
- DNA Repair Mechanisms
- Renal and related cancers
- Peptidase Inhibition and Analysis
- Inflammatory Bowel Disease
- Reproductive Biology and Fertility
East China Normal University
2005-2025
Chongqing Medical University
2011-2024
The First Affiliated Hospital, Sun Yat-sen University
2020-2024
Sun Yat-sen University
2020-2024
Sichuan University
2024
West China Hospital of Sichuan University
2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2024
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2023
Chinese University of Hong Kong, Shenzhen
2023
First People's Hospital of Chongqing
2023
Abstract As the lowest organisms possessing T cells, fish are instrumental for understanding cell evolution and immune defense in early vertebrates. This study established Nile tilapia models suggests that cells play a critical role resisting Edwardsiella piscicida infection via cytotoxicity essential IgM + B response. CD3 CD28 monoclonal antibody crosslinking reveals full activation of requires first secondary signals, while Ca 2+ –NFAT, MAPK/ERK, NF‐ κ B, mTORC1 pathways collectively...
The braking mechanisms to protect the host from tissue damage and inflammatory disease caused by an overexuberant immune response are common in many T cell subsets. However, negative regulation of responses detailed not well understood early vertebrates. In current study, using a Nile tilapia (Oreochromis niloticus) model, we investigated suppression immunity IL-10. Tilapia encodes evolutionarily conserved IL-10, whose expression lymphocytes is markedly induced during primary adaptive...
Enoblituzumab, an immunotherapeutic agent targeting CD276, shows both safety and efficacy in activating T cells oligodendrocyte-like against various cancers. Preclinical studies mouse models suggest that therapies CD276 may outperform PD1/PD-L1 blockade. However, data from indicate a significant non-responsive population to anti-CD276 treatment, with the mechanisms of resistance still unclear. In this study, we evaluate activity antibodies chemically-induced murine model head neck squamous...
TGF-β has been demonstrated to promote tumor metastasis, and the regulatory mechanisms are poorly understood. Here, we report role of USP2a in promoting metastasis by facilitating TGF-β-triggered signaling. interacts with TGFBR1 TGFBR2 upon stimulation removes K33-linked polyubiquitin chains from Lys502 TGFBR1, recruitment SMAD2/3. Simultaneously, phosphorylates Ser207/Ser225 USP2a, leading disassociation SMAD2/3 TGFBR1. The phosphorylation SMAD2 is positively correlated human biopsies,...
Abstract As fish constitute the first evolutionary group with primordial T cells, they are of importance for understanding origin and evolution adaptive immunity. Yet, knowledge about how ancestral cells function remains limited. Therefore, teleost model Nile tilapia ( Oreochromis niloticus ) was used in this study to investigate regulatory mechanisms T‐cell immunity fish. We identified an evolutionarily conserved canonical NF‐κB signaling pathway tilapia, which participates primary immune...
Abstract As an immune checkpoint, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) suppresses the activation, proliferation, and effector function of T cells, thus preventing overexuberant response maintaining homeostasis. However, whether how this checkpoint functions in early vertebrates remains unknown. In current study, using a Nile tilapia (Oreochromis niloticus) model, we investigated suppression cell by CTLA-4 bony fish. Tilapia is constitutively expressed lymphoid tissues, its...
Gastric cancer (GC) is one of the leading causes cancer-associated mortality worldwide. The aim present study was to investigate mechanism microRNA-4295 (miR-4295), which regulates cisplatin (DDP)-induced apoptosis in GC cells through leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1)-mediated epidermal growth factor receptor (EGFR)/phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway. Two cell lines were selected, with highest expression miR-4295 lowest...
Calcium ion (Ca2+) is a widespread and primitive second messenger that regulates physiological cell functions in almost all life beings. Ca2+ influx-induced NFAT activation essential for T function adaptive immunity. However, whether how signaling modulates immunity early vertebrates, especially nontetrapods, remains largely unknown. To address these questions, Nile tilapia (Oreochromis niloticus) model was employed to investigate the regulation of ancestral by Ca2+-NFAT jawed fish. In...
Abstract Osteosarcoma (OS) is the most prevalent type of primary malignant bone cancer and currently lacks effective targeted treatments. Increasing evidence indicates that SOX2 overexpression a driver OS. By screening small-molecule kinase inhibitor library, we identified AKT as essential for robust expression in OS cells. was found to be frequently overexpressed positively correlated with protein levels. We demonstrated has no effect on transcription but promotes stability....
Although bony fish have CD8+ T cells, the mechanisms by which these early-evolved cytotoxic cells combat intracellular pathogens remain unclear. In present study, using Nile tilapia as a model, we investigated detailed function, mechanism, and evolutionary pattern concerning cells. By depleting they are found essential in combating Edwardsiella piscicida infection. Using siRNA interference, propose that unlike strategy predominantly relying on perforin/granzyme mammals, T-cell effector...
Ovarian cancer remains the most lethal gynecological malignancy, largely owing to its chemotherapy resistance and high recurrence rate. Emerging evidence has linked aberrant expression of SOX2, a transcription factor that is important in development maintenance stem cell state, with chemoresistance poor prognosis ovarian patients. In this study, we aimed elucidate mechanisms drive SOX2 cells. By examining multiple lines panel clinical tumor samples, observed broad overexpression tumors. To...
Abstract Background Epigenetic regulation plays an important role in the development and progression of nasopharyngeal carcinoma (NPC). However, epigenetic mechanisms underlying NPC metastasis remains poorly understood. We aimed to find functional genes which regulate identify therapeutic targets for treatment. Methods Bisulfite pyrosequencing was used analyze zinc finger protein 582 ( ZNF582 ) methylation tissues cell lines. Quantitative reverse transcription‐polymerase chain reaction...
The integration of metabolic programs with T cell signaling establishes a molecular foundation for immune metabolism. As key regulator, GSK3β's activity is dynamically modulated by phosphorylation at Ser9 and Tyr216. However, the contribution these sites on metabolism-driven response remains unclear. Using tilapia mouse models, we investigated regulation GSK3β metabolism its evolutionary variation. In tilapia, activation induces signaling, linking to both glycolysis oxidative (OXPHOS)....
Transforming growth factor-β1 (TGF-β1) can suppress the activation, proliferation, and function of many T-cell subsets, protecting organisms from inflammatory autoimmune disease caused by an overexuberant immune response. However, whether how TGF-β1 regulates immunity in early vertebrates remain unknown. Here, using a Nile tilapia (Oreochromis niloticus) model, we investigated suppression response teleost species. Tilapia encodes evolutionarily conserved TGF-β1, expression which lymphocytes...
Bone marrow mesenchymal stem cells (BMSCs) on the repair of spinal cord injury (SCI) in rats as well role transforming growth factor‑β (TGF-β)/Smads signaling pathway were investigated. Rat BMSCs and astrocyte-spinal cords (ASCs) isolated cultured vitro, cell purity was detected by flow cytometry. ASCs co-cultured with TGF-β1, + TGF‑β1, respectively, grouped accordingly, conventionally included into control group. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5‑diphenyltetrazoliumbromide (MTT) assay...
Abstract Methyltransferase like 13 (METTL13), a kind of methyltransferase, is implicated in protein binding and synthesis. The upregulation METTL13 has been reported variety tumors. However, little was known about its potential function head neck squamous cell carcinoma (HNSCC) so far. In this study, we found that significantly upregulated HNSCC at both mRNA level. Increased negatively associated with clinical prognosis. And markedly affected cellular phenotypes vivo vitro. Further mechanism...
Abstract Despite the advances in study on osmotic physiology bony fish, mechanism by which immune system, especially T-cell immunity, adapts and responds to stress remains unknown. In current study, we investigated response of T cells hyperosmotic fish Nile tilapia (Oreochromis niloticus). As a euryhaline was able adapt wide range salinities; however, hypertonic caused inflammation excessive activation. Furthermore, increased expression IL-17A cells, upregulated transcription factor RORα,...