A5006134461- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- MicroRNA in disease regulation
- Muscle Physiology and Disorders
- Cardiomyopathy and Myosin Studies
- Complement system in diseases
- Silk-based biomaterials and applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Renal and related cancers
- Optical measurement and interference techniques
- Blood disorders and treatments
- Aquaculture disease management and microbiota
- Congenital heart defects research
- Cell Adhesion Molecules Research
- Genetic Syndromes and Imprinting
- Animal Genetics and Reproduction
- Collagen: Extraction and Characterization
- Hemoglobinopathies and Related Disorders
- Congenital Diaphragmatic Hernia Studies
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
Erasmus University Rotterdam
2019-2025
Erasmus MC
2019-2025
Erasmus MC Cancer Institute
2023
Hacettepe University
2020-2021
Palmetto Hematology Oncology
2019
University Medical Center Freiburg
2019
Reprogenetics
2014
Istanbul Medeniyet University
2014
The GATA2 transcription factor is a pivotal regulator of hematopoiesis. Disruptions in the gene drive severe hematologic abnormalities and are associated with an increased risk myelodysplastic syndromes acute myeloid leukemia; however, mechanisms underlying pathophysiology deficiency remain still unclear. We developed two different mouse models that based on serial limiting donor cell transplantation (aged) haploinsufficient cells mirror symptoms deficiency. Similar to what has been observed...
Nephronophthisis (NPHP) is one of the most common genetic causes CKD; however, underlying abnormalities have been established in <50% patients. We performed genome-wide analysis followed by targeted resequencing a Turkish consanguineous multiplex family and identified canonic splice site mutation ANKS6 associated with an NPHP-like phenotype. Furthermore, we four additional variants cohort 56 unrelated patients diagnosed CKD due to nephronophthisis, chronic GN, interstitial nephritis, or...
Abstract Gata2 is a key transcription factor required to generate Haematopoietic Stem and Progenitor Cells (HSPCs) from haemogenic endothelium (HE); misexpression of leads haematopoietic disorders. Here we deleted conserved enhancer (i4 enhancer) driving pan-endothelial expression the zebrafish gata2a showed that Gata2a for HE programming by regulating runx1 second orthologue, gata2b . By 5 days, homozygous Δi4/Δi4 larvae normal numbers HSPCs, recovery mediated Notch signalling in HE....
The differentiation of hematopoietic stem cells (HSCs) is tightly controlled to ensure a proper balance between myeloid and lymphoid cell output. GATA2 pivotal transcription factor required for generation maintenance HSCs. expressed throughout development, but because early embryonic lethality in mice, its role during adult hematopoiesis incompletely understood. Zebrafish contains 2 orthologs GATA2: Gata2a Gata2b, which are different types. We show that the mammalian functions split these...
Abstract Desmin is a muscle-specific intermediate filament protein that has fundamental role in muscle structure and force transmission. Whereas human desmin encoded by single gene, two paralogs ( desma desmb ) exist zebrafish. Desma show differential spatiotemporal expression during zebrafish embryonic larval development, being similarly expressed skeletal until hatching, after which of shifts to gut smooth muscle. We generated knockout (KO) mutant lines carrying loss-of-function mutations...
Abstract The first hematopoietic stem cells (HSCs) are formed through endothelial-to-hematopoietic transition (EHT) during embryonic development. transcription factor GATA2 is a crucial regulator of EHT and HSC function throughout life. Because patients with haploinsufficiency have inborn mutations, prenatal defects likely to influence disease In mice, Gata2 (Gata2+/−) reduces the number functionality progenitor (HSPCs) generated EHT. However, HSPC pool heterogeneous mechanisms underlying...
The GATA2 transcription factor is a pivotal regulator of hematopoiesis. Disruptions in the gene drive severe hematologic abnormalities and are associated with an increased risk myelodysplastic syndromes acute myeloid leukemia; however, mechanisms underlying pathophysiology deficiency remain still unclear. We developed two different mouse models that based on serial limiting donor cell transplantation (aged) haploinsufficient cells mirror symptoms deficiency. Similar to what has been observed...
Abstract Hematopoietic stem cells (HSCs) are tightly controlled to keep a balance between myeloid and lymphoid cell differentiation. Gata2 is pivotal hematopoietic transcription factor required for HSC generation maintenance. We generated zebrafish mutant the mammalian orthologue, gata2b . found that in adult zebrafish, both neutrophilic- monocytic lineage Single transcriptome analysis revealed defect present Gata2b deficient arise most immature progenitor (HSPC) compartment this population...
Background: Patients with GATA2 mutations have up to 80% chance develop a myeloid malignancy. MonoMAC and Emberger syndromes are deficiency characterized by monocytopenia, B, NK cell lymphopenia predisposition acute leukemia (AML). Although the disease initiating mutation is known, it unclear how these patients hematological malignancies. Aims: In our research we use both mouse zebrafish models of Gata2 haploinsufficiency describe their phenotypes study mechanisms malignant transformation....
Abstract Haematopoietic stem and progenitor cells (HSPCs) maintain the vertebrate blood system throughout life their emergence from haemogenic endothelium (HE) is regulated by transcription factors such as Gata2. Here we deleted a conserved enhancer (i4 enhancer) driving pan-endothelial expression of gata2a showed that Gata2a required for HE programming regulating runx1 second zebrafish Gata2 orthologue, gata2b . By 5 days, homozygous Δi4/Δi4 larvae normal numbers HSPCs, recovery mediated...
Background: GATA2 is the most frequently mutated gene in childhood AML. These patients have germline mutations one allele of and a high predisposition for MDS/AML. Also, mouse, Gata2 required embryonic HSC generation survival complete deletion lethal at day (E)10, while haploinsufficiency results severe reduction formation HSCs. The first HSCs are formed E10.5 through process called endothelial‐to‐hematopoietic transition (EHT) from specialized hemogenic endothelial cells dorsal aorta...
Abstract Desmin is a muscle-specific intermediate filament protein that has fundamental role in muscle structure and force transmission. Whereas human desmin encoded by single gene, two paralogs ( desma desmb ) exist zebrafish. Desma show differential spatiotemporal expression during zebrafish embryonic larval development, being similarly expressed skeletal until hatching, after which of shifts to gut smooth muscle. We generated knockout (KO) mutant lines carrying loss-of-function mutations...