A5073008506- Pharmacogenetics and Drug Metabolism
- Drug-Induced Hepatotoxicity and Protection
- Sepsis Diagnosis and Treatment
- Glutathione Transferases and Polymorphisms
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Drug Transport and Resistance Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Liver physiology and pathology
- 3D Printing in Biomedical Research
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Cancer therapeutics and mechanisms
- Environmental Toxicology and Ecotoxicology
- Adrenal Hormones and Disorders
- Estrogen and related hormone effects
- Carcinogens and Genotoxicity Assessment
- Pesticide Exposure and Toxicity
- Cancer Cells and Metastasis
- Cancer, Stress, Anesthesia, and Immune Response
- Sphingolipid Metabolism and Signaling
- Eicosanoids and Hypertension Pharmacology
- Kruppel-like factors research
- Pharmacological Receptor Mechanisms and Effects
- Genetics, Aging, and Longevity in Model Organisms
Université de Rennes
2010-2024
Centre Hospitalier Universitaire de Rennes
2021-2024
Inserm
2010-2023
Foie, Métabolisme, Cancer
2006-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2023
Nutrition, métabolismes et cancer
2021-2023
Université Bretagne Loire
2018
Hôpital Pontchaillou
2009-2015
Laboratoire de Recherche sur la Croissance Cellulaire, la Réparation et la Régénération Tissulaires
2005
Centre National de la Recherche Scientifique
2001
Most human hepatocyte cell lines lack a substantial set of liver-specific functions, especially major cytochrome P450 (P450)-related enzyme activities, making them unrepresentative in vivo hepatocytes. We have used the HepaRG cells, derived from hepatocellular carcinoma, which exhibit high differentiation pattern after 2 weeks at confluency to determine whether they could mimic hepatocytes for drug metabolism and toxicity studies. show that when passaged low density, these cells reversed an...
The human hepatoma HepaRG cells are able to differentiate in vitro into hepatocyte-like and express various liver-specific functions, including the major cytochromes P450. This study was aimed determine whether differentiated retained their specific functional capacities for a long time period at confluence. We show that expression of transcripts encoding CYP1A2, 2B6, 3A4, 2E1, several phase II antioxidant enzymes, membrane transporters, organic cation transporter 1 bile salt export pump,...
In this article, we present a liver-kidney co-culture model in micro fluidic biochip. The liver was modeled using HepG2/C3a and HepaRG cell lines the kidney MDCK lines. To demonstrate synergic interaction between both organs, investigated effect of ifosfamide, an anticancerous drug. Ifosfamide is prodrug which metabolized by to isophosforamide mustard, active metabolite. This metabolism process also leads formation chloroacetaldehyde, nephrotoxic metabolite acrolein urotoxic one. biochips...
Abstract Current developments in tissue engineering and microtechnology fields allow the use of microfluidic biochip as microtools for vitro investigations. In present study, we describe behavior HepG2/C3a cells cultivated a poly(dimethylsiloxane) (PDMS) coupled to perfusion system. Cell culture 96 h including 72 provoked 24 delay cell growth compared plate cultures. Inside biochip, few apoptosis, necrosis were detected along 3D organization was observed. Regarding hepatic metabolism,...
Human hepatocellular carcinoma (HCC) heterogeneity promotes recurrence and resistance to therapies. Recent studies have reported that HCC may be derived not only from adult hepatocytes hepatoblasts but also hepatic stem/progenitors. In this context, HepaRG cells represent a suitable cellular model study stem/progenitor cancer the retrodifferentiation of tumor-derived hepatocyte-like cells. Indeed, they differentiate into hepatocyte- biliary-like Moreover, (HepaRG-tdHep) both through...
Objective: The liver is an early target organ in sepsis, severe and septic shock, contributing to multiple failure, both lipopolysaccharide gut-derived catecholamines are implicated the occurrence of hepatocellular dysfunction. Treatment shock involves administration vasoactive agents such as exogenous or vasopressin order reestablish blood pressure. As a prelude clinical application, we tested hypothesis that could modulate lipopolysaccharide-induced inflammatory response function human...
Glutathione transferases (GST) are essentially known as enzymes that catalyse the conjugation of glutathione to various electrophilic compounds such chemical carcinogens, environmental pollutants, and antitumor agents. However, this protein family is also involved in metabolism endogenous which play critical roles regulation signaling pathways. For example, lipid peroxidation product 4-hydroxynonenal (4-HNE) prostaglandin 15-deoxy-Δ12,14-prostaglandin J(2) (15d-PGJ(2)) metabolized by GSTs...
Dimethyl sulfoxide (DMSO) is used to sustain or favor hepatocyte differentiation in vitro. Thus, DMSO the protocol of HepaRG cells that present closest drug-metabolizing enzyme activities primary human hepatocytes culture. The aim our study clarify its influence on liver-specific gene expression. For purpose, we performed a large-scale analysis (gene expression and histone modification) determine global role exposure during process cells. addition drives upregulation genes mainly regulated...
Knowledge about the impact of epinephrine on outcome in venoarterial (VA) extracorporeal membrane oxygenation (ECMO) patients is limited, and existing data are conflicting. We conducted a retrospective cohort study 1500 bed tertiary university hospital. Five hundred eighty-nine VA-ECMO were analysed. The median age was 57 years [47-65], 68% male. major indications for ECMO post-cardiotomy cardiogenic shock (CS) (38%) medical CS (36%). Two sixty-two (44.5%) received alone or associated with...
Roquefortine, a cyclopeptide derived from the diketopiperazine cyclo(Trp-dehydroHis), is secondary metabolite produced by several Penicillium species. It has been reported to cause neurotoxic effect and inhibit Gram-positive bacteria growth. The mechanisms responsible for its toxicity metabolism are still unknown. In this study, we investigated interaction of roquefortine with mammalian cytochromes P450. Roquefortine rat human liver P450 was monitored difference UV-vis spectroscopy. found...
Pifithrin α (PFTα) is a chemical compound that inhibits p53-mediated gene activation and apoptosis. It has also been recently shown to alter metabolism of carcinogenic polycyclic aromatic hydrocarbons (PAHs). This led us examine the effect PFTα on activity cytochrome P-450 (CYP) 1 isoforms, known metabolize PAHs, such as benzo( )pyrene (BP), into mutagenic metabolites. We report caused potent inhibition CYP1-related measured by ethoxyresorufin O-deethylase in CYP1-containing MCF-7 cells...
Abstract Glutathione transferases (GST) are phase II enzymes catalyzing the detoxification of endogenous noxious compounds and xenobiotics. They also regulate phosphorylation activities MAPKinases in a catalytic-independent manner. Previous studies have demonstrated regulation JNK-dependent pathway by GSTP1/2. Considering crucial role JNK early steps hepatocyte cell cycle, we sought to determine whether GSTP1/2 were essential for proliferation following partial hepatectomy (PH). Using...
Glutathione S-transferases (GSTs) detoxify toxic molecules by conjugation with reduced glutathione and regulate cell signaling. Single nucleotide polymorphisms (SNPs) of GST genes have been suggested to affect functions thus increase the risk human hepatocellular carcinoma (HCC). As GSTA1 is expressed in hepatocytes rs3957357C>T (TT) SNP known downregulate mRNA expression, aims this study were: (i) explore relationship between TT occurrence HCC; (ii) measure expression HCCs. For that...