A5004557448- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Plant and animal studies
- CRISPR and Genetic Engineering
- Lichen and fungal ecology
- Immunotherapy and Immune Responses
- Ecology and Vegetation Dynamics Studies
- Advanced Biosensing Techniques and Applications
- Transgenic Plants and Applications
- Metabolism and Genetic Disorders
- Trace Elements in Health
- Genetic diversity and population structure
- Immunodeficiency and Autoimmune Disorders
- Amino Acid Enzymes and Metabolism
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Immune responses and vaccinations
- vaccines and immunoinformatics approaches
- Biological and pharmacological studies of plants
- Advanced biosensing and bioanalysis techniques
Southern Medical University
2020-2024
Nanfang Hospital
2020-2021
Shenyang Agricultural University
2013
The extent to which AIRRs differ among and within individuals remains elusive. Via ultra-deep repertoire sequencing of 22 25 tissues in three cynomolgus macaques, respectively, we identified 84 114 novel IGHV TRBV alleles, confirming 72 (85.71%) 100 (87.72%) them. heterogeneous V gene usage patterns were influenced, turn, by genetics, isotype (for BCRs only), tissue group, tissue. A higher proportion intragroup shared clones the intestinal than those other suggests a close intra-intestinal...
Antibody repertoire sequencing enables researchers to acquire millions of B cell receptors and investigate these molecules at the single-nucleotide level. This power resolution in studying humoral responses have led its wide applications. However, most studies were conducted with a limited number samples. Given extraordinary diversity, assessment key features large sample set is demanded. Thus, we collect systematically analyze 2,152 high-quality heavy-chain antibody repertoires. Our study...
The sequence upstream of the antibody variable region (antibody [AUS]) consists a 5' untranslated (5' UTR) and preceding leader region. variations in AUS affect engineering PCR based quantification may also be implicated mRNA transcription translation. However, diversity AUSs remains elusive. Using rapid amplification cDNA ends high-throughput repertoire sequencing technique, we acquired full-length for human, rhesus macaque, cynomolgus mouse, rat. We designed bioinformatics pipeline...
Abstract The adaptive immune receptor repertoire consists of the entire set an individual’s BCRs and TCRs is believed to contain a record prior responses potential for future immunity. Analyses TCR repertoires via deep learning (DL) methods have successfully diagnosed cancers infectious diseases, including coronavirus disease 2019. However, few studies used DL analyze BCR repertoires. In this study, we collected IgG H chain Ab from 276 healthy control subjects 326 patients with various...
Detailed knowledge of the diverse immunoglobulin germline genes is critical for study humoral immunity. Hundreds alleles have been discovered by analyzing antibody repertoire sequencing (Rep-seq or Ig-seq) data via multiple novel allele detection tools (NADTs). However, performance these NADTs through sequences with intrinsic somatic hypermutations (SHMs) unclear. Here, we developed a tool to simulate repertoires integrating full spectrum features an such as gene usage, junctional...
The antibody repertoire is a critical component of the adaptive immune system and believed to reflect an individual’s history current status. Delineating has advanced our understanding humoral immunity, facilitated discovery, showed great potential for improving diagnosis treatment disease. However, no tool date effectively integrated big Rep-seq data prior knowledge functional antibodies elucidate remarkably diverse repertoire. We developed dataset Analysis Platform with Integrated Database...
Abstract The sequence upstream of antibody variable region (Antibody Upstream Sequence, or AUS) consists 5’ untranslated (5’ UTR) and two leader regions, L-PART1 L-PART2. variations in AUS affect the efficiency PCR amplification, mRNA translation, subsequent PCR-based quantification as well engineering. Despite their importance, diversity AUSs has long been neglected. Utilizing rapid amplification cDNA ends (5’RACE) high-throughput repertoire sequencing (Rep-Seq) technique, we acquired...
Abstract Antibody repertoire refers to the totality of superbly diversified antibodies within an individual cope with vast array possible pathogens. Despite this extreme diversity, same clonotype, namely public clones, have been discovered among individuals. Although some clones could be explained by antibody convergence, in naïve or virus-neutralizing from not infected people were also discovered. All these findings indicated that might occur random and they exert essential functions....
Abstract Detailed knowledge of the diverse immunoglobulin germline genes is critical for study humoral immunity. Hundreds alleles have been discovered by analyzing antibody repertoire sequencing (Rep-seq or Ig-seq) data via multiple novel allele detection tools (NADTs). However, performance these NADTs through sequences with intrinsic somatic hypermutations (SHMs) unclear. Here, we developed a tool to simulate repertoires integrating full spectrum features an such as gene usage, junctional...
To establish a simple method to detect four ATP7B gene mutations in Wilson disease using allele-specific PCR(AS-PCR) with whole blood polymerase chain reaction.Four PCR primers specific for the mutations(G2333T, C2850T, G2855A, G2975T) were designed, and was optimized screen samples. The amplified products mutation separated agarose gel electrophoresis pattern of point allele types. Exons 8, 12 13 PCR, amplification sequenced confirm mutation.The detection by AS-PCR accomplished 100%...
COVID-19 has swept across the world, causing widespread epidemics and millions of life lost worldwide. After infected with 2019-nCoV, body quickly mobilizes innate immune response produces type Ⅰ interferon (IFN-Ⅰ). IFN-Ⅰ plays an important role in virus clearance early stage disease. This article reviews recognition after infection interaction between 2019-nCoV IFN-Ⅰ, which would be conductive to understanding pathogenesis antiviral treatment COVID-19. © 2021 Chinese Medical Association