A5065943236- Hepatocellular Carcinoma Treatment and Prognosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- DNA Repair Mechanisms
- Colorectal Cancer Treatments and Studies
- Bacterial Genetics and Biotechnology
- Liver Disease Diagnosis and Treatment
- DNA and Nucleic Acid Chemistry
- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Pancreatic and Hepatic Oncology Research
- Gallbladder and Bile Duct Disorders
- Hepatitis B Virus Studies
- Multiple and Secondary Primary Cancers
- Pediatric Hepatobiliary Diseases and Treatments
- Cancer Genomics and Diagnostics
- Esophageal Cancer Research and Treatment
- Gastric Cancer Management and Outcomes
- RNA and protein synthesis mechanisms
- Porphyrin Metabolism and Disorders
- Photodynamic Therapy Research Studies
- Bladder and Urothelial Cancer Treatments
- Hepatitis C virus research
- Lung Cancer Treatments and Mutations
- Sphingolipid Metabolism and Signaling
- Immune cells in cancer
The University of Texas MD Anderson Cancer Center
2021-2025
Qatif Central Hospital
2025
NTT Medical Center
2016-2025
The University of Tokyo
2015-2024
Kyushu University
1983-2018
Kyoto University
2018
New Generation University College
2018
Seoul National University
2018
University of Oslo
2018
Fujieda Municipal General Hospital
2015-2017
8-Oxoguanine (8-oxo-7, 8-dihydroguanine) is produced in DNA, as well nucleotide pools of cells, by active oxygen species normally formed during cellular metabolic processes. can pair with cytosine and adenine nucleotides at almost equal efficiencies, transversion mutation ensues. Human cells contain enzyme activity, which hydrolyzes 8-oxo-dGTP to 8-oxo-dGMP, this responsible for preventing misincorporation 8-oxoguanine into DNA. We purified particular human physical homogeneity determined a...
8-Oxoguanine nucleotide can pair with cytosine and adenine nucleotides at almost equal efficiencies. Once 8-oxodGTP is formed in the cellular pool, this mutagenic incorporated into DNA would cause transversion mutations. The MutT protein of Escherichia coli possesses enzyme activity to hydrolyze corresponding nucleoside monophosphate thus may be responsible for preventing occurrence such Here we show that human cell has an specifically hydrolyzing a fashion similar seen protein. 8-oxodGTPase...
Pol III, a subassembly of Escherichia coli DNA polymerase III holoenzyme lacking only the auxiliary beta subunit, was purified to homogeneity by an improved procedure. This assembly consists nine different polypeptides, likely in 1:1 stoichiometry: catalytic core (pol III) alpha (132 kDa), epsilon (27 and theta (10 six subunits: tau (71 gamma (52 delta (35 delta' (33 chi (15 psi (12 kDa). The behaves on gel filtration as particle about 800 kDa, indicating content two each subunits. A new...
The alpha subunit (140 kDa) of DNA polymerase III (pol III) holoenzyme has been purified to near-homogeneity from a plasmid-carrying Escherichia coli strain which overproduced the about 20-fold. Pol core (containing only alpha, epsilon, and theta subunits), produced at twice normal level, was also in good yield. isolated activity, is completely inhibited by 10 mM N-ethylmaleimide or 150 KCl as observed pol holoenzyme. an apparent turnover number 7.7 nucleotides polymerized per s, compared 20...
The polymerase subunit (alpha) of Escherichia coli DNA III holoenzyme and the 3'----5' exonuclease (epsilon) are each less active separately than together in core (an assembly alpha, epsilon, theta subunits). In a complex formed from purified alpha epsilon subunits, activity increased 2-fold, that 10- to 80-fold. alpha-epsilon contains one subunits as does core. Stimulation is due mainly greatly affinity for 3'-hydroxyl terminus, resulting binding by subunit. Proofreading course synthesis...
Occurrence of the transversion mutation A.T to C.G is specifically enhanced in Escherichia coli mutT mutants. With aid cloned gene, MutT protein, which has a molecular mass 15 kilodaltons, was overproduced and purified near homogeneity. The protein catalyzes hydrolysis dGTP dGMP. dGDP GTP were also hydrolyzed by but at lower rate than seen with dGTP. No other deoxynucleoside triphosphates hydrolyzed. Using poly(dA).(dT)20 as template-primer, we investigated misincorporation dGMP, dCMP, dAMP...
A 1.6-kilobase-pair DNA fragment derived from the Escherichia coli chromosome was analyzed by Tn3 transposon insertion and deletion mapping to locate a mutator gene, dnaQ (mutD), rnh gene that codes for RNase H. When strong promoter, PL of lambda phage, placed at right- left-side cloned fragment, protein H, respectively were overproduced. These results suggested two genes are transcribed in opposite directions their promoters located narrow region between genes. Nucleotide sequence analysis...
Abstract The role of sphingosine 1-phosphate (S1P) in liver fibrosis or inflammation was not fully examined human. Controversy exists which S1P receptors, S1P1 and S1P3 vs S1P2, would be importantly involved its mechanism. To clarify these matters, 80 patients who received resection for hepatocellular carcinoma 9 metastatic tumor were enrolled. metabolism analyzed background, non-tumorous tissue. mRNA levels kinase 1 (SK1) but SK2 increased livers with stages 3–4 compared to those 0–2 normal...
Sickle Cell Hepatopathy is a multifactorial liver disease that can have an impact on half of patients with Disease (SCD), cryptic and puzzling connection appearing to be associating (SCD) Autoimmune Hepatitis (AIH), repeatedly yielding diagnostic neglect delay, bringing about disastrous consequences. This case introduces you 41-year-old single Saudi male known who was accidentally diagnosed advanced cirrhosis by pure chance during laparoscopic cholecystectomy. study will draw attention the...
The level of O6-methylguanine-DNA methyltransferase activity in a human cell line carrying 1.1-kilobase cDNA fragment was about 50 times higher than that found ordinary methyltransferase-proficient (Mer+) lines (Hayakawa, H., Koike, G., and Sekiguchi, M. (1990) J. Mol. Biol. 213, 739-747). Taking advantage this overproduction, the enzyme purified to apparent physical homogeneity biochemical properties investigated. A single polypeptide with molecular weight approximately 25,000 detected on...
By in vitro recombination we have constructed hybrid plasmids capable of complementing a conditional lethal mutator mutation, dnaQ49, Escherichia coli K12. The dnaQ+ consist full-length pBR322 DNA and 1.5-kilobase fragment derived from the E. chromosome. Specific labeling plasmid-encoded proteins by maxicell method revealed that insert codes for two proteins, one whose molecular weight is 25,000 [the 25-kilodalton (kDal) protein] other 21,000 (the 21-kDal protein). Because insertion gamma...
Hepatocellular carcinoma (HCC) commonly develops in patients with liver fibrosis; these patients, the blood levels of lysophosphatidic acid (LPA) and its generating enzyme autotaxin (ATX) increase fibrosis stage. We aimed to examine potential relevance ATX LPA HCC. Fifty-eight HCC who underwent surgical treatment were consecutively enrolled study. Among receptors HCC, higher LPA2 mRNA correlated poorer differentiation, LPA6 microvascular invasion, which suggested a malignant increased...
DNA polymerase III; core, pol 111 core; ssDNA, single-stranded
DNA polymerase IV (Pol IV) is one of three translesion polymerases in Escherichia coli. A mass spectrometry study revealed that single-stranded DNA-binding protein (SSB) lysates prepared from exponentially-growing cells has a strong affinity for column-immobilized Pol IV. We found purified SSB binds directly to pull-down assay, whereas SSBΔC8, mutant lacking the C-terminal tail, failed interact with These results show interaction between and mediated by tail SSB. When activity was tested on...
Abstract Most potent mutators heretofore detected in Escherichia coli are associated with defects epsilon subunit of DNA polymerase III, encoded by the dnaQ gene. To elucidate role alpha subunit, catalytic polymerase, maintaining high fidelity replication, we isolated a mutator mutant, mutation (dnaE173) which resides on dnaE gene, encoding subunit. The dnaE173 mutant was unable to grow salt-free L broth at temperatures exceeding 44.5 degrees C and exhibited an increased frequency...