A5019682947- Reproductive Biology and Fertility
- Air Quality and Health Impacts
- Microtubule and mitosis dynamics
- Atmospheric chemistry and aerosols
- Cellular Mechanics and Interactions
- Veterinary Oncology Research
- Primate Behavior and Ecology
- Pluripotent Stem Cells Research
- Bird parasitology and diseases
- Air Quality Monitoring and Forecasting
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Preterm Birth and Chorioamnionitis
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Neonatal and fetal brain pathology
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Neonatal and Maternal Infections
- CRISPR and Genetic Engineering
- Atmospheric aerosols and clouds
- Sirtuins and Resveratrol in Medicine
- Biocrusts and Microbial Ecology
- Digital Holography and Microscopy
- Teratomas and Epidermoid Cysts
University of Florida
2023-2024
Korea Research Institute of Bioscience and Biotechnology
2019-2024
Chungbuk National University
2014-2021
University of Ulsan
2020-2021
Asan Medical Center
2020-2021
Ulsan College
2020-2021
Chonnam National University
2020
Yeungnam University
2015
If no fertilization occurs at an appropriate time after ovulation, oocyte quality deteriorates rapidly as a process called postovulatory aging. Because the aging of oocytes has detrimental effects on embryo development and offspring, many efforts have been made to prevent Here we showed that quercetin prevented decline in during oocytes. Quercetin treatment reduced aging-induced morphological changes reactive oxygen species accumulation. Moreover, attenuated aging-associated abnormalities...
Abstract Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) plays an important role in RNA processing via m 6 A modification of pre-mRNA or pre-miRNA. However, the functional and relationship between hnRNPA2/B1 early embryonic development are unclear. Here, we found that is crucial for by virtue regulating specific gene transcripts. HnRNPA2/B1 was localized to nucleus cytoplasm during subsequent development, starting at fertilization. Knockdown delayed after 4-cell stage blocked...
Abstract. The UNIfied Partitioning-Aerosol phase Reaction (UNIPAR) model was integrated into the Comprehensive Air quality Model with extensions (CAMx) to process secondary organic aerosol (SOA) formation by capturing multiphase reactions of hydrocarbons (HCs) in regional scales. SOA growth simulated using a wide range anthropogenic HCs, including 10 aromatics and linear alkanes different carbon lengths. atmospheric processes biogenic HCs (isoprene, terpenes, sesquiterpene) were for major...
Actin polymerization is essential for various stages of mammalian oocyte maturation, including spindle migration, actin cap formation, polar body extrusion, and cytokinesis. The heterodimeric actin-capping protein (CP) an element the cytoskeleton. It binds to fast-growing (barbed) ends filaments plays roles in actin-mediated cellular processes. However, CP maturation are poorly understood. We investigated mouse oocytes found that correct asymmetric migration extrusion. mainly localized...
Dynamic actin reorganization is the main driving force for spindle migration and asymmetric cell division in mammalian oocytes. It has been reported that various nucleators including Formin-2 are involved polarization of division. In mammals, formin family comprised 15 proteins. However, their individual roles and/or have not elucidated yet. this study, we employed a newly developed inhibitor proteins, small molecule homology 2 domains (SMIFH2), to assess functions mouse oocyte maturation....
Abstract The dynamic polymerization and depolymerization of actin filaments is essential for various cellular processes such as cell migration, rotation, cytokinesis, mammalian oocyte maturation. Tropomodulin 3 (Tmod3) binds to the slow-growing (pointed) ends filament, thereby protecting filament from depolymerization. However, roles Tmod3 in maturation remain elusive. mRNA protein present at all stages mouse mainly localized cytoplasm appears enriched near chromosome during By knocking down...
Abstract. The UNIfied Partitioning-Aerosol phase Reaction (UNIPAR) model was established on the Comprehensive Air quality Model with extensions (CAMx) to process Secondary Organic Aerosol (SOA) formation by capturing multiphase reactions of hydrocarbons (HCs) in regional scales. SOA growth simulated using a wide range anthropogenic HCs including ten aromatics and linear alkanes different carbon-lengths. atmospheric processes biogenic (isoprene, terpenes, sesquiterpene) were for major...
Abstract. Branched alkanes represent a significant proportion of hydrocarbons emitted in urban environments. To accurately predict the secondary organic aerosol (SOA) budgets environments, these branched should be considered as SOA precursors. However, potential to form from diverse under varying environmental conditions is currently not well understood. In this study, Unified Partitioning Aerosol Phase Reaction (UNIPAR) model extended formation via multiphase reactions various alkanes....
What is the function of Spindlin 1 (Spin1) in metaphase II stage oocytes pigs?Depletion Spin1 induces spontaneous oocyte activation and overexpression causes multinuclear formation through induction DNA damage porcine oocytes.Little known about embryos. In mouse oocytes, specifically expressed during gametogenesis essential for meiotic resumption. somatic cells, promotes cancer cell proliferation activates WNT/T-cell factor signaling.After knockdown (KD) or MII-stage MII maintenance was...
SUMMARY Oocyte meiosis involves a unique asymmetric division involving spindle movement from the central cytoplasm to cortex, followed by polar body extrusion. ROCK is Rho‐GTPase effector involved in various cellular functions somatic cells as well oocyte meiosis. was previously shown promote actin organization phosphorylating several downstream targets, including LIM domain kinase (LIMK), phosphorylated cofilin (p‐cofilin), and myosin light chain (MLC). In this study, we investigated roles...
Background/Aim: Picrasma quassioides (P. quassioides) is used in traditional Asian medicine widely for the treatment of anemopyretic cold, eczema, nausea, loss appetite, diabetes mellitus, hypertension etc. In this study we aimed to understand effect P. ethanol extract on SiHa cervical cancer cell apoptosis. Materials and Methods: The extract-induced apoptosis was analyzed using MTT assay, fluorescence microscopy, flow cytometry western blotting. Results: induced cellular by increasing...
Tropomyosins are actin-binding cytoskeletal proteins that play a pivotal role in regulating the function of actin filaments muscle and non-muscle cells; however, roles tropomyosins mouse oocytes unknown. This study investigated expression functions tropomyosin (Tpm3) during meiotic maturation oocytes. Tpm3 mRNA was detected at all developmental stages protein localized cortex germinal vesicle breakdown stages. However, overall fluorescence intensity immunostaining markedly decreased...
Protein phosphatase 2A regulatory subunit B55α (PP2A-B55α) has been studied in mitosis. However, its functions mammalian meiosis and early embryonic development remain unknown. Here, we report that PP2A-B55α is critical for mouse oocyte preimplantation embryo development. Knockdown of oocytes led to abnormal asymmetric division, disordered spindle dynamics, defects chromosome congression, an increase aneuploidy, induction the DNA damage response. Moreover, knockdown fertilized zygotes...
Dynamic reorganization of the actin cytoskeleton is fundamental to a number cellular events, and various actin-regulatory proteins modulate polymerization depolymerization. Adenylyl cyclase-associated (CAPs), highly conserved monomer-binding proteins, have been known promote disassembly by enhancing actin-severing activity ADF/cofilin protein family. In this study, we found that CAP1 regulated remodeling during mouse oocyte maturation. Efficient maturation essential for asymmetric division...
Particulate matter (PM) is a general atmospheric pollutant released into the air by an anthropogenic and naturally derived mixture of substances. Current studies indicate that fine dust can result in different health defects, including endothelial dysfunction, asthma, lung cancer, cardiovascular diseases, uterine leiomyoma, deterioration sperm quality, overall birth impairment. However, most prominent effects PM 10 (diameter < μM) exposure on female reproductive system, especially...
Particulate matter (PM) is a major air pollutant that affects human health worldwide. PM can pass through the skin barrier, thus causing diseases such as heat rash, allergic reaction, infection, or inflammation. However, only few studies have been conducted on cytotoxic effects of exposure large-scale animals. Therefore, herein, we investigated whether and how rhesus macaque fibroblasts. Rhesus fibroblasts were treated with various concentrations PM10 (1, 5, 10, 50, 100 μg/mL) incubated for...
Polymethylmethacrylate (PMMA) has been used in many products, such as acrylic glass, and is estimated to reach 5.7 million tons of production per year by 2028. Thus, nano-sized PMMA particles the environment are highly likely due weathering process. However, information on hazards nanoplastics, including mammals, especially reproductive toxicity action mechanism, scarce. Herein, we investigated effect nanoplastics female system mice embryos during pre-implantation. The treated plastic (10,...
Abstract Background Cynomolgus monkeys ( Macaca fascicularis ) are essential in biomedical research, including reproductive studies. However, the application of human estimated foetal weight (EFW) formulas using ultrasonography (USG) these non‐human primates is not well established. Objectives This study aims to evaluate applicability EFW for estimating cynomolgus at approximately 130 days gestation. Methods Our involved nine pregnant monkeys. We measured parameters, biparietal diameter,...
Measurements of the three-dimensional (3D) structure spermatozoon are crucial for study developmental biology and evaluation in vitro fertilization. Here, we present 3D label-free imaging individual perform quantitative analysis bovine, porcine, mouse spermatozoa morphologies using refractive index tomography. Various morphological biophysical properties were determined, including internal structure, volume, surface area, concentration, dry matter mass spermatozoon. Furthermore, Holstein...
Zinc plays an essential role in mammalian oocyte maturation, fertilization, and early embryogenesis, depletion of zinc impairs cell cycle control, asymmetric division, cytokinesis oocyte. We report that zinc, via the actin nucleator Spire, acts as regulator cytoskeleton remodeling during mouse maturation fertilization. Depletion impaired cortical cytoplasmic formation. Spire is colocalized with zinc-containing vesicles its finger-containing Fab1, YOTB, Vac 1, EEA1 (FYVE) domain. Improper...
Abstract. Branched alkanes represent a significant proportion of hydrocarbons emitted in urban environments. To accurately predict the SOA budgets environments, these branched should be considered as precursors. However, potential to form from diverse under varying environmental conditions is currently not well understood. In this study, Unified Partitioning Aerosol Phase Reaction (UNIPAR) model extended formation via multiphase reactions various alkanes. Simulations with UNIPAR model, which...
Objective: This study was conducted to investigate the roles of LIM kinases (LIMK1 and LIMK2) during porcine early embryo development. We checked mRNA expression patterns localization LIMK1/2 evaluate their characterization. further explored function in developmental competence relationship between actin assembly cell junction integrity, specifically first cleavage compaction.Methods: Pig ovaries were transferred from a local slaughterhouse within 1 h cumulus oocyte complexes (COCs)...