Putative myogenic and endothelial (myo-endothelial) cell progenitors were identified in the interstitial spaces of murine skeletal muscle by immunohistochemistry immunoelectron microscopy using CD34 antigen. Enzymatically isolated cells characterized fluorescence-activated sorting on basis surface antigen expression, sorted as a CD34+ CD45− fraction. Cells this fraction ∼94% positive for Sca-1, mostly negative (<3% positive) CD14, 31, 49, 144, c-kit, FLK-1. The CD34+/45− formed...

A new method for strength-training of rat hindlimb muscles, comparable to human weight lifting, is compared with sprint training by a treadmill. The apparatus that can induce rats perform squats was designed. Squat composed isotonic high-intensity, short-duration, and graded overload exercises. After 60 tnin one bout all-out squat training, serum creatine kinase activities were markedly increased in the group (P < 0.001), but no significant changes observed group. These responses reflected...

We used a rat model of weight lifting to examine the serial biochemical and morphological changes following muscle fiber hyperplasia during 14 days exercise. [3H]thymidine [14C]leucine labeling were determine in cellular mitotic activity level amino acid uptake myosin synthesis. Morphological assessed with light transmission electron microscopy, whereas proliferation cells was evaluated immunohistochemically 5-bromo-2'-deoxyuridine (BrdU). The intensity exercise degree damage monitored by...

Recent studies have shown that skeletal muscle-derived stem cells (MDSCs) can give rise to several cell lineages after transplantation. However, the potential therapeutic uses of MDSCs, functional significance transplanted tissue, and vasculogenesis, myogenesis, reconstitution other tissues yet be investigated in detail. In addition, relationship between MDSCs mesenchymal bone marrow is interest.We developed a severe-damage model mouse tibialis anterior muscle with large deficit nerve...

The influence of an anabolic androgenic steroid (AAS) on thymidine and amino acid uptake in rat hindlimb skeletal muscles during 14 days after a single exhaustive bout weight lifting was determined. Adult male rats were divided randomly into Control or Steroid groups. Nandrolone decanoate administered to the group 1 wk before exercise bout. [ 3 H]thymidine C]leucine labeling used determine serial changes cellular mitotic activity, uptake, myosin synthesis. Serum creatine kinase (CK) as...

The differentiation potential of skeletal muscle-derived stem cells (MDSCs) after in vitro culture and vivo transplantation has been extensively studied. However, the clonal multipotency MDSCs yet to be fully determined. Here, we show that single CD34-/CD45- (skeletal double negative [Sk-DN]) exhibit can give rise myogenic, vasculogenic, neural cell lineages cell-derived sphere implantation culture. Muscles from green fluorescent protein (GFP) transgenic mice were enzymatically dissociated...

The purpose of the present study was to compare myogenic response hindlimb muscles in young (14–20 wk age) and old (&gt;120 rats with a single exhaustive bout heavy resistance weight lifting. [ 3 H]thymidine 14 C]leucine labeling were monitored for up 2 after exercise estimate serial changes mitotic activity level amino acid uptake myosin synthesis. Histological, histochemical, immunohistochemical [anti-5-bromo-2′-deoxyuridine determination genes (MyoD)] analyses whole analysis...

Loss of vital functions in the somatic motor and sensory nervous systems can be induced by severe peripheral nerve transection with a long gap following trauma. In such cases, autologous grafts have been used as gold standard, expectation activation proliferation graft-concomitant Schwann cells associated their paracrine effects. However, there are limited number suitable sites available for harvesting autografts due to unavoidable sacrifice other healthy functions. To overcome this problem,...

Losses in vital functions of the somatic motor and sensory nervous system are induced by severe long-gap peripheral nerve transection injury. In such cases, autologous grafts gold standard treatment, despite unavoidable sacrifice other healthy functions, whereas prognosis is not always favorable. Here, we use human skeletal muscle-derived stem cells (Sk-SCs) to reconstitute function after long nerve-gap Muscles samples were obtained from amputated legs 9 patients following unforeseen...

The purpose of this study was to determine whether fiber hyperplasia occurs in the rat plantaris muscle during postnatal weeks 3–20. Total number, obtained via nitric acid digestion method, increased by 28% early rapid growth phase (3–10 weeks), whereas number branched fibers consistently low. Whole-muscle mitotic activity and amino uptake levels showed an inverse relationship increase total number. expression MyoD mRNA (RT-PCR) decreased from 3 20 age, as did detection anti-BrdU-...

BackgroundCellular cardiomyoplasty for myocardial infarction has been developed using various cell types. However, complete differentiation and/or trans-differentiation into cardiomyocytes have never occurred in these transplant studies, whereas functional contributions were reported.Methods and ResultsSkeletal muscle interstitium-derived CD34+/CD45− (Sk-34) cells purified from green fluorescent protein transgenic mice by flowcytometory. Cardiac of Sk-34 was examined vitro clonal culture...

BACKGROUND.: Postoperative neurogenic bladder dysfunction is a major complication of radical hysterectomy for cervical cancer and mainly caused by unavoidable damage to the branch pelvic plexus (BBPP) associated with colateral blood vessels. Thus, we attempted reconstitute disrupted BBPP vessels using skeletal muscle-derived multipotent stem cells that show synchronized reconstitution capacity vascular, muscular, peripheral nervous systems. METHODS.: Under pentobarbital anesthesia,...

The hierarchical relationship of skeletal muscle-derived multipotent stem cells sorted as CD34(+)/CD45(-) (Sk-34) and CD34(-)/CD45(-) (Sk-DN) cells, which have synchronized reconstitution capacities for blood vessels, peripheral nerves, muscle fibers, was examined. Expression Sca-1 CD34 (typical state freshly isolated Sk-34 cells) in Sk-DN examined using vitro culture vivo cell implantation. sequentially expressed during showing self-maintenance and/or self-renewal-like behavior, are thus...

We herein describe a surgical technique for the repair of complete tear pectoralis major (PM) tendon using endobuttons to strengthen initial fixation. Five male patients (3 judo players, 1 martial arts player, and body builder) were treated within 2 weeks sustaining PM tendon. Average age at surgery follow-up period 28.4 years (range, 23-33) 28.8 months 24-36). A rectangular bone trough (about × 4 cm) was created on humerus insertion distal The stump introduced into this trough, fixed...

Skeletal muscle makes up 40-50% of body mass, and is thus considered to be a good adult stem cell source for autologous therapy. Although, several stem/progenitor cells have been fractionated from mouse skeletal showing high potential therapeutic use, it unclear whether this the case in human. Differentiation human muscle-derived (Sk-Cs) was examined. Samples (5-10 g) were obtained abdominal leg muscles 36 patients (age, 17-79 years) undergoing prostate cancer treatment or amputation...

Tissue regeneration requires both growth factor and extracellular matrix such as collagen, serving a scaffold for cell growth. We established FNCBD-VEGF121, consisting of the fibronectin collagen-binding domain (FNCBD) vascular endothelial (VEGF) 121, investigated its properties.FNCBD-VEGF121 specifically bound to gelatin type I, II, III, IV, V collagen. This collagen-bound FNCBD-VEGF121 captured soluble VEGF receptor 2 (VEGFR-2)/Fc chimeric protein. Cell growth-promoting activity was almost...

Background. Postoperative damage of the urethral rhabdosphincter (URS) and neurovascular bundle (NVB) is a major operative complication radical prostatectomy. It generally recognized to be caused by unavoidable surgical muscle-nerve-blood vessel units around urethra. We attempted treat this using skeletal muscle-derived stem cells, which are able reconstitute units. Methods. Cells were enzymatically extracted sorted flow cytometry as CD34+/45− (Sk-34) CD34−/45− (Sk-DN) cells from green...

Qualitative changes in the peripheral motor system were examined using Young, Adult, Middle-aged and Old-aged rats order to assess before after appearance of sarcopenia symptoms. Significant loss muscle mass strength, slow-type fiber grouping with a innervated nerve fibers used as typical markers sarcopenia. Dynamic twitch tetanus tension evoked electromyogram (EEMG) measured via electrical stimulation through sciatic under anesthesia our force-distance transducer sciatectomy. Digital...