A5005049809- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Nitric Oxide and Endothelin Effects
- Bone health and treatments
- Click Chemistry and Applications
- Proteoglycans and glycosaminoglycans research
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
University of Oxford
2019-2024
Science Oxford
2020
Oxfam
2019
Abstract Oligonucleotides that target mRNA have great promise as therapeutic agents for life-threatening conditions but suffer from poor bioavailability, hence high cost. As currently untreatable diseases come within the reach of oligonucleotide therapies, new analogues are urgently needed to address this. With this in mind we describe reduced-charge oligonucleotides containing artificial LNA-amide linkages with improved gymnotic cell uptake, RNA affinity, stability and potency. To construct...
Triazole linkages (TLs) are mimics of the phosphodiester bond in oligonucleotides with applications synthetic biology and biotechnology. Here we report RuAAC-catalyzed synthesis a novel 1,5-disubstituted triazole (TL2) dinucleoside phosphoramidite as well its incorporation into compare DNA polymerase replication competency other TL analogues. We demonstrate that TL2 has superior kinetics to these analogues is accurately replicated by polymerases. Derived structure–biocompatibility...
The combination of amide coupling with standard oligonucleotide synthesis enables assembly reduced charge chimeric gapmer antisense oligonucleotides that trigger an efficient RNase H response while improving serum lifetime and cellular uptake.
Carbamate-LNA oligonucleotides have improved biophysical properties for theraputic applications.
The modification of DNA or RNA backbones is an emerging technology for therapeutic oligonucleotides, synthetic biology and biotechnology. Despite a plethora reported artificial backbones, their vast potential not fully utilised. Limited accessibility remains major bottleneck the wider application backbone-modified oligonucleotides. Thus, variety readily accessible robust methods introduction into oligonucleotides are urgently needed to utilise full in therapeutics,
Abstract Oligonucleotides that target mRNA have great promise as therapeutic agents for life-threatening conditions but suffer from poor bioavailability, hence high cost. As currently untreatable diseases come within the reach of oligonucleotide therapies, new analogues are urgently needed to address this. With this in mind we developed reduced-charge oligonucleotides containing artificial LNA-amide linkages with improved gymnotic cell uptake, RNA affinity, stability and potency. To...