A5000799938- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Methane Hydrates and Related Phenomena
- RNA Interference and Gene Delivery
- Electron Spin Resonance Studies
- Epigenetics and DNA Methylation
- X-ray Diffraction in Crystallography
- Spacecraft and Cryogenic Technologies
- Magnetism in coordination complexes
- Crystallization and Solubility Studies
- CO2 Sequestration and Geologic Interactions
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Lanthanide and Transition Metal Complexes
- Genetics and Neurodevelopmental Disorders
- Photonic Crystals and Applications
- Cancer therapeutics and mechanisms
- Metal complexes synthesis and properties
- RNA and protein synthesis mechanisms
- DNA and Biological Computing
- Advanced Manufacturing and Logistics Optimization
University of Oxford
2017-2022
Durham University
2017
Institute of Navigation
1963
Abstract Oligonucleotides that target mRNA have great promise as therapeutic agents for life-threatening conditions but suffer from poor bioavailability, hence high cost. As currently untreatable diseases come within the reach of oligonucleotide therapies, new analogues are urgently needed to address this. With this in mind we describe reduced-charge oligonucleotides containing artificial LNA-amide linkages with improved gymnotic cell uptake, RNA affinity, stability and potency. To construct...
The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. ability measure 2-10 nm particularly important: deformations arising from protein binding commonly fall within this range, but reliable measurement such for a conformational ensemble remains significant challenge. Using several techniques, we show that electron paramagnetic resonance (EPR) spectroscopy oligonucleotides spin-labelled with...
A wide variety of approaches have become available for the fabrication nanomaterials with increasing degrees complexity, precision, and speed while minimizing cost. Their quantitative characterization, however, remains a challenge. Analytical methods to better inspect validate structure composition large nanoscale objects are required optimize their applications in diverse technologies. Here, we describe single-molecule fluorescence-based strategies relying on photobleaching multiple-color...
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Abstract Oligonucleotides that target mRNA have great promise as therapeutic agents for life-threatening conditions but suffer from poor bioavailability, hence high cost. As currently untreatable diseases come within the reach of oligonucleotide therapies, new analogues are urgently needed to address this. With this in mind we developed reduced-charge oligonucleotides containing artificial LNA-amide linkages with improved gymnotic cell uptake, RNA affinity, stability and potency. To...