A5026136145- Plant tissue culture and regeneration
- Biofuel production and bioconversion
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Food composition and properties
- Insect Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Cancer-related molecular mechanisms research
- Blockchain Technology Applications and Security
- Plant-Microbe Interactions and Immunity
- Enzyme Production and Characterization
- Circular RNAs in diseases
- Glutathione Transferases and Polymorphisms
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Nitrogen and Sulfur Effects on Brassica
- Cellular Mechanics and Interactions
- Genomics, phytochemicals, and oxidative stress
- Plant Virus Research Studies
- Soybean genetics and cultivation
- Cloud Data Security Solutions
SRM Institute of Science and Technology
2024
The University of Adelaide
2008-2022
Western University
2022
Wellcome Centre for Cell-Matrix Research
2022
University of Manchester
2022
ARC Centre of Excellence in Plant Energy Biology
2016-2017
Australian Research Council
2016
Australian Centre for Plant Functional Genomics
2010-2012
Agriculture Victoria
1997
Transcription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application molecular plant breeding. We investigated possibility modulating transcriptional regulation drought cold responses agriculturally important species, wheat barley, with view increase frost tolerance. Transgenic barley plants were generated showing constitutive (double 35S) drought-inducible (maize Rab17) expression...
Cell walls in commercially important cereals and grasses are characterized by the presence of (1,3;1,4)-β-d-glucans. These polysaccharides beneficial constituents human diets, where they can reduce risk hypercholesterolemia, type II diabetes, obesity colorectal cancer. The biosynthesis cell wall (1,3;1,4)-β-d-glucans Poaceae is mediated, part at least, cellulose synthase-like CslF family genes. Over-expression barley CslF6 gene under control an endosperm-specific oat globulin promoter...
The ability to increase cellulose content and improve the stem strength of cereals could have beneficial applications in lodging producing crops with higher for biofuel feedstocks. Here, such potential is explored commercially important crop barley through manipulation synthase genes (CesA). Barley plants transformed primary cell wall (PCW) secondary (SCW) (HvCesA) cDNAs driven by CaMV 35S promoter, were analysed growth morphology, transcript levels, content, strength, tissue morphology...
The recent characterization of the polysaccharide composition papillae deposited at barley cell wall during infection by powdery mildew pathogen, Blumeria graminis f. sp. hordei (Bgh), has provided new targets for generation enhanced disease resistance. role callose in papilla-based penetration resistance crop species is largely unknown because genes involved observed accumulation have not been identified unequivocally. We employed both comparative and functional genomics approaches to...
Engineering of plant protection in cereals requires well characterized tissue-specific and wounding/pathogen-inducible promoters for targeted expression pathogen responsive resistance genes. We describe the isolation seven wheat rice defensin genes expressed early developing grain during germination, two developmental stages that are particularly vulnerable to pathogens insects. Comparison three-dimensional (3D) models these PRPI defensins indicated variations spatial architectures could...
Summary Homeodomain leucine zipper class I ( HD ‐Zip I) transcription factors TF s) play key roles in the regulation of plant growth and development under stresses. Functions Ta HDZ ipI‐2 gene isolated from endosperm developing wheat grain were revealed. Molecular characterization protein included studies its dimerisation, protein– DNA interactions activation properties using pull‐down assays, in‐yeast methods transient expression assays cells. The analysis functions was performed transgenic...
Summary Two putative endosperm‐specific rice genes, OsPR602 and OsPR9a , were identified from database searches. The promoter regions of these genes isolated, transcriptional promoter:β‐glucuronidase (GUS) fusion constructs stably transformed into barley. GUS expression patterns revealed that promoters active in early grain development both barley, showed strongest endosperm transfer cells during the stages filling. was similar but, exclusively differed timing activation relative to rice. In...
(1,3;1,4)-β-Glucan is a non-cellulosic polysaccharide required for correct barley grain fill and plant development, with industrial relevance in the brewing functional food sector. Barley grains contain higher levels of (1,3;1,4)-β-glucan compared to other small cereals this influences their end use, having undesirable effects on distilling beneficial linked human health. HvCslF6 main gene contributing biosynthesis grain. Here, transcriptional regulation was investigated using an in-silico...
The TaPR61 gene from bread wheat encodes a lipid transfer protein (LTP) with hydrophobic signal peptide, predicted to direct the apoplast. Modelling of revealed presence an internal cavity which can accommodate at least two molecules. full-length gene, including promoter sequence orthologue, was cloned BAC library Triticum durum. Quantitative RT-PCR analysis and TdPR61 mainly in grain. A transcriptional promoter–GUS fusion stably transformed into wheat, barley, rice. strongest GUS expression...
Summary An HD‐Zip IV gene from wheat, TaGL9 , was isolated using a Y1H screen of cDNA library prepared developing wheat grain. has an amino acid sequence distinct other reported members the family. The 3′ untranslated region used as probe to isolate genomic clone homologue BAC Triticum durum L. cv. Langdon. full‐length containing 3‐kb‐long promoter designated TdGL9H1 . Spatial and temporal activity examined promoter‐GUS fusion constructs in transgenic barley rice plants. Whole‐mount...
Hull-less barley is increasingly offering scope for breeding grains with improved characteristics human nutrition; however, recalcitrance of hull-less cultivars to transformation has limited the use these varieties. To overcome this limitation, we sought develop an effective system using cultivar Torrens. Torrens yielded a efficiency 1.8%, modified Agrobacterium method. This method was used over-express genes encoding synthases important dietary fiber component, (1,3;1,4)-β-glucan...
Blockchain has a strong capacity to monitor and retain educational records. The paperless future yet become reality, even with the ability digitally generate documents. Physical copy of records are still regularly printed which makes them susceptible document fraud. Thus, issue fake certificates academic risen drastically. In this paper, we have made reliable verification method avoid frauds. idea presented here is developed over Hyperledger. University or Educational Institute responsible...
Objective: Angiotensin II (Ang II)-induced endothelial dysfunction plays an important role in the pathogenesis of cardiovascular diseases such as systemic hypertension, cardiac hypertrophy and atherosclerosis. Recently, long noncoding RNAs (lncRNAs) have been shown to play essential pathobiology diseases; however, effect Ang on lncRNAs coding expression cells has not evaluated. Accordingly, we sought evaluate profiles following treatment with II. Methods: Human umbilical vein (HUVECs) were...
Introduction: Autophagy maintains cellular homeostasis by degradation of unnecessary proteins for their recycling and reuse. MicroRNAs (miRNAs) regulate various biological processes including autophagy. MiRNA autophagy both endothelial function, however, role miRNAs in function is unknown. Methods Results: To understand the autophagy, was genetically inhibited via ATG7 (autophagy-related gene 7) silencing Human Umbilical Vein Endothelial Cells (HUVECs) then a miRNA array performed, which...