A5071524792- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Eosinophilic Esophagitis
- T-cell and B-cell Immunology
- Drug-Induced Adverse Reactions
- CAR-T cell therapy research
- Mast cells and histamine
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Plant Virus Research Studies
- Nuts composition and effects
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Animal Disease Management and Epidemiology
- Plant and Fungal Interactions Research
- Computational Drug Discovery Methods
- Immune cells in cancer
- Protein Kinase Regulation and GTPase Signaling
- Agricultural safety and regulations
- Sepsis Diagnosis and Treatment
- Adipose Tissue and Metabolism
Assaf Harofeh Medical Center
2015-2025
International AIDS Vaccine Initiative
2021-2024
Scripps Research Institute
2021-2024
Tel Aviv University
2018-2021
Ben-Gurion University of the Negev
2011-2012
Hackensack University Medical Center
2009-2012
Thomas Jefferson University
2008
Howard Hughes Medical Institute
2001
Yale University
2001
University of Massachusetts Chan Medical School
2001
The c-Jun N-terminal kinase (JNK) signal transduction pathway is activated in response to the exposure of cells environmental stress. Components JNK signaling interact with JIP1 scaffold protein. located neurites primary hippocampal neurons. However, stress, accumulates soma together and phosphorylated c-Jun. Disruption Jip1 gene mice by homologous recombination prevented activation caused excitotoxic stress anoxic vivo vitro. These data show that protein a critical component MAP-kinase pathway.
NK cells play an important role in the early immune response to cancer. The NKp44 activating receptor is only natural cytotoxicity that expressed exclusively by primate cells, yet its cellular ligands remain largely unknown. Proliferating cell nuclear Ag (PCNA) overexpressed cancer cells. In this study, we show recognizes PCNA. Their interaction inhibits function through NKp44/ITIM. physical of and PCNA enabled recruitment target immunological synapse. We demonstrate promotes survival...
Ambiguities exist regarding the diagnosis of tree-nut allergy, necessitating either their elimination or performance oral food challenges (OFCs).To examine coincidences allergies among tree-nuts and improve diagnostic testing to minimize need for OFC.Eighty-three patients prospectively evaluated walnut, pecan, cashew, pistachio, hazelnut, almond allergy. A history previous reactions was obtained, standardized skin prick tests (SPTs) using finely ground solution basophil activation (BAT) were...
Abstract Background Multiple studies suggest a key role for gut microbiota in IgE-mediated food allergy (FA) development, but to date, none has studied it the persistent state. Methods To characterize composition and short-chain fatty acid (SCFAs) profiles associated with major groups, we recruited 233 patients FA including milk ( N = 66), sesame 38), peanut 71), tree nuts 58), non-allergic controls 58). DNA was isolated from fecal samples, 16S rRNA gene sequences were analyzed. SCFAs stool...
Oral immunotherapy (OIT) is a treatment option for patients with milk, egg, and peanut allergy, but data on the efficacy safety of cashew OIT are limited.A cohort 50 cashew-allergic aged ≥4 years, who were consecutively enrolled into (target dose 4000 mg protein) between 4/2016 12/2019. Fifteen continued elimination served as observational controls. Co-allergy to pistachio walnut was determined. Full desensitization rate associated immunological changes in both groups compared. Patients...
Summary Background The prevalence of sesame food allergy ( SFA ) has increased over recent years, with the potential anaphylactic reactions upon exposure. Oral challenge OFC remains diagnostic standard, yet its implementation may be risky. Commercial skin prick tests SPT have a low sensitivity. Investigation alternate methods is warranted. Objective To evaluate utility and basophil activation test BAT for diagnosis. Methods Eighty‐two patients suspected completed an open to or reported...
Abstract Studies examining the long‐term effect of oral immunotherapy in food‐allergic patients are limited. We investigated cow's milk‐allergic patients, >6 months after completion ( n = 197). Questionnaires, skin prick tests, and basophil activation assays were performed. Of 195 contacted, 180 (92.3%) consuming milk protein regularly. Half experienced adverse reactions, mostly mild. Thirteen (6.7%) required injectable epinephrine. Higher reaction rate was associated with more...
Abstract Background The prevalence of sesame food allergy (SFA) is increasing worldwide with the potential anaphylactic reactions upon exposure. Utility specific component IgE testing as an alternative to oral challenge (OFC), diagnostic standard, being investigated. Methods Patients ( n = 42) suspected SFA completed open OFC sesame. Diagnostic included serum levels Ses i 1‐specific IgE, skin prick test high‐protein extract, and basophil reactivity (% induced CD63 expression) for each...
The MHC-matched, minor histocompatibility Ag (miHA)-mismatched B10.BR-->CBA strain combination has been used to elucidate the immunobiology of graft-vs-host disease (GVHD) following allogeneic bone marrow transplantation. Studies conducted in 1980s had established that B10.BR CD8+ T cells were capable mediating GVHD absence CD4+ cells, and unable induce lethal disease. In more recent studies with this model, we detected etiological discrepancies previously published results, which suggested...
Abstract NKp46 is a primary activating receptor of NK cells that involved in lysis target by cells. Previous studies showed the membrane-proximal domain (NKp46D2) retained binding to its ligands and lysis. We studied NKp46D2 using peptide-based epitope mapping approach identified an NKp46D2-derived linear inhibited NKp46-mediated The epitope, designated as pep4 (aa 136–155), interacted with NKp46, was presence pep4. Through modeling mutagenesis, we could be homodimerization. R145 D147...
The generation of broadly neutralizing antibodies (bnAbs) to specific HIV epitopes the Envelope (Env) is one cornerstones vaccine research. current animal models we use have been unable reliable produce a antibody response, with exception cows. Cows rapidly and reliably produced CD4 binding site response by homologous prime boosting native-like Env trimer. In small other engineered immunogens previously able focus responses bnAb V2-apex region Env. Here, immunized two groups cows (n=4)...
Elicitation of HIV broadly neutralizing antibodies (bnAbs) is challenging because unmutated bnAb precursors are rare and seldom bind envelope glycoprotein (Env) trimers. One strategy to initiate responses use germline-targeting (GT) immunogens with high affinity bnAb-class precursor B cells then shepherd maturation booster that successively look more like native Env. In a mouse model where the frequency VRC01-precursor (VRC01gHL) mimics humans, we show following GT Env trimer protein prime,...
Vaccination strategies aimed at maturing broadly neutralizing antibodies (bnAbs) from naïve precursors are hindered by unusual features that characterize these Abs, including insertions and deletions (indels). Longitudinal studies of natural HIV infection cases shed light on the complex processes underlying bnAb development have suggested a role for superinfection as potential enhancer neutralization breadth. Here we describe potent lineage was elicited two founder viruses to inform vaccine...