A5009078847- Estrogen and related hormone effects
- Hemoglobin structure and function
- Protein Kinase Regulation and GTPase Signaling
- Cholesterol and Lipid Metabolism
- Phytochemicals and Antioxidant Activities
- Hormonal Regulation and Hypertension
- Mitochondrial Function and Pathology
- Retinoids in leukemia and cellular processes
- Ion channel regulation and function
- Pharmacogenetics and Drug Metabolism
- Reproductive System and Pregnancy
- Erythrocyte Function and Pathophysiology
- Drug Transport and Resistance Mechanisms
- Cytokine Signaling Pathways and Interactions
- Receptor Mechanisms and Signaling
- PI3K/AKT/mTOR signaling in cancer
- Nitric Oxide and Endothelin Effects
- Nutrition, Genetics, and Disease
- Antioxidant Activity and Oxidative Stress
- Phytoestrogen effects and research
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Neuroscience of respiration and sleep
- Lipid metabolism and biosynthesis
Roma Tre University
2009-2024
Fondazione Santa Lucia
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2024
Marconi University
2008-2015
Institute of Biostructure and Bioimaging
2009-2013
In-Q-Tel
2009
Istituto Nazionale di Fisica Nucleare, Sezione di Roma Tre
1998
Bristol-Myers Squibb (United States)
1998
Bristol-Myers Squibb (Germany)
1997
Sapienza University of Rome
1986-1994
A fraction of the nuclear estrogen receptor alpha (ERalpha) is localized to plasma membrane region 17beta-estradiol (E2) target cells. We previously reported that ERalpha a palmitoylated protein. To gain insight into molecular mechanism residence at membrane, we tested both role palmitoylation and impact E2 stimulation on localization. The cancer cell lines expressing transfected or endogenous human (HeLa HepG2, respectively) nonpalmitoylable Cys447Ala mutant in HeLa cells were used as...
Estrogens induce cell proliferation in target tissues by stimulating progression through the G1 phase of cycle. Activation cyclin D(1) gene expression is a critical feature this hormonal action. The existence rapid/nongenomic estradiol-regulated protein kinase C (PKC-alpha) and extracellular signal-regulated (ERK) signal transduction pathways, their cross talk, role played DNA synthesis transcription have been studied herein human hepatoma HepG2 cells. 17Beta-estradiol was found to rapidly...
Abstract l-T3 and l-T4 activated the Na+/H+ exchanger of L-6 myoblasts, with a fast nongenomic mechanism, both in steady state when cells undergo acid loading ammonium chloride. Monitored intracellular pH-sensitive fluorescent probe 2′,7′-bis(carboxyethyl)-5(6)-carboxyfluorescein, activation appeared to be initiated at plasma membrane, because T3-agarose reproduced effect l-T3, triiodothyroacetic acid, hormone analog previously shown inhibit membrane actions thyroid hormone, blocked action...
Naringenin (Nar) is a component of fruits and vegetables associated with healthful benefits, such as in osteoporosis, cancer, cardiovascular diseases. These protective effects have been linked Nar antiestrogenic well estrogenic activities. Previous studies indicate that impaired estrogen receptor (ER) α signaling by interfering ERα-mediated activation ERK phosphoinositide 3-kinase pathways the absence at transcriptional level. The present evaluated hypothesis these antagonistic occur level...
The sex steroid hormone 17β-estradiol (E2) upregulates the levels of neuroglobin (NGB), a new neuroprotectant globin, to elicit its neuroprotective effect against H2O2-induced apoptosis. Several mechanisms could be proposed justify NGB involvement in E2 prevention stress-induced apoptotic cell death. Here, we evaluate ability modulate intracellular localization and interaction with mitochondrial cytochrome c following toxicity. Present results demonstrate that is expressed nuclei,...
We reported previously in HepG2 cells that estradiol induces cell cycle progression throughout the G1-S transition by parallel stimulation of both PKC-alpha and ERK signaling molecules. The analysis cyclin D1 gene expression showed only MAP kinase pathway was involved. Here, presence rapid/nongenomic, estradiol-regulated, PI3K/AKT signal transduction pathway, its modulation levels tumor suppressor PTEN, cross-talk with involvement DNA synthesis promoter activity have all been studied cells....
Abstract Quercetin has potentially beneficial effects on disease prevention, including cancer. An intriguing issue regarding the mechanisms of action quercetin is ability this drug to modulate estrogen receptor (ER) activities. In a previous study, we demonstrated that elicited apoptosis through an ERα‐dependent mechanism. However, contribution ERβ in quercetin‐induced remains elusive. Here, report quercetin, at nutritionally relevant concentrations, mimicked 17β‐estradiol (E2)‐induced...
Although discovered in 2000, neuroglobin (Ngb) functions are still uncertain. A contribution to the role played by Ngb neurons could certainly derive from identification of endogenous modulators. Here, we evaluate possibility that be regulated 17β-estradiol (E2) signaling both SK-N-BE human neuroblastoma cell line and mouse hippocampal neurons. 1 nM E2 rapidly induced a 300% increase levels models. The effect was specific, being not testosterone or dihydrotestosterone. E2-induced requires...
17β-Estradiol (E 2 ) mediates a wide variety of complex biological processes determining the growth and development reproductive tract as well nonreproductive tissues male female individuals. While E effects on system, bone, cardiovascular system are quite established, less is known about how it affects physiology other tissues. Skeletal muscle tissue that expected to be responsive since both isoforms estrogen receptor (ER-α ER-β) expressed. Significant sex-related differences have been...
Adipocyte differentiation plays an important role in bone remodeling due to secretory factors that can directly modulate osteoblast and osteoclast, thus affecting overall mass skeletal integrity. Excessive adipocyte within marrow microenvironment lead decreased mass, eventually causing osteoporosis. Mechanical of marrow, including fluid shear, maintains the balance during remodeling. However, how mechanical cues interact with long noncoding RNA (lncRNA) regulating remains unexplored. In this...
Abstract Recently, we reported that human neuroglobin (NGB) is a new player in the signal transduction pathways lead to 17 β -estradiol (E2)-induced neuron survival. Indeed, E2 induces mitochondria enhancement of NGB level, which turn impairs activation pro-apoptotic cascade. Nowadays, existence similar pathway activated by non-neuronal cells completely unknown. Here, role E2-induced upregulation tumor reported. induced dose- and time-dependent manner MCF-7, HepG2, SK-N-BE, HeLa transfected...
Sexual dimorphism, detectable in vascular smooth muscle cells freshly isolated from aorta of male and female rats, is associated with a different susceptibility to radiation‐induced apoptosis. In this work we investigated the mechanism underlying difference discovered that, comparison rats show adhesion‐associated resistance apoptosis, so called anoikis resistance. This apparently due more adhering phenotype, characterized by well organized actin microfilament cytoskeleton an increased...
Abstract Flavonoids have been described as health‐promoting, disease‐preventing dietary components. In vivo and in vitro experiments also support a protective effect of flavonoids to reduce the incidence certain hormone‐responsive cancers. particular, our previous results indicate that flavanone naringenin (Nar), decoupling estrogen receptor α (ERα) action mechanisms, drives cancer cells apoptosis. Because these studies were conducted absence endogenous hormone 17β‐estradiol (E2),...