A5077937218- Estrogen and related hormone effects
- Effects and risks of endocrine disrupting chemicals
- Retinoids in leukemia and cellular processes
- Receptor Mechanisms and Signaling
- Cytokine Signaling Pathways and Interactions
- Toxic Organic Pollutants Impact
- Innovations in Medical Education
- Mast cells and histamine
- Steroid Chemistry and Biochemistry
- Inflammatory mediators and NSAID effects
- Chemokine receptors and signaling
- SARS-CoV-2 and COVID-19 Research
- Peroxisome Proliferator-Activated Receptors
- Birth, Development, and Health
- Carcinogens and Genotoxicity Assessment
- Reproductive System and Pregnancy
- COVID-19 and Mental Health
- Hormonal and reproductive studies
- Protein Kinase Regulation and GTPase Signaling
- Hypothalamic control of reproductive hormones
- Respiratory viral infections research
- Problem and Project Based Learning
- Pharmacogenetics and Drug Metabolism
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
Quinnipiac University
2019-2025
Rainbow Babies & Children's Hospital
2023-2025
International League Against Epilepsy
2021-2024
Campbell University
2012-2019
Newcastle upon Tyne Hospitals NHS Foundation Trust
2014
Great North Children's Hospital
2014
Research Triangle Park Foundation
2009
Duke University
1999-2008
The Hamner Institutes for Health Sciences
2008
Duke University Hospital
1999-2007
Abstract The human estrogen receptor α (ERα) and the recently identified ERβ share a high degree of amino acid homology; however, there are significant differences in regions these receptors that would be expected to influence transcriptional activity. Consequently, we compared mechanism(s) by which regulate target gene transcription, evaluated cellular consequences coexpression both ER subtypes. Previously, it has been determined ERα contains two distinct activation domains, ERα-AF-1...
Recent clinical studies estimate that 60-70% of human ovarian and breast cancers overexpress the estrogen receptor (ER). However, despite established mitogenic effects in these tumors, proliferative markers hormone action are limited. In current study, we report growth stimulatory cytokine stromal cell-derived factor 1 (SDF-1) is a bona fide target ERalpha-positive cancer cells. Notably, estradiol treatment BG-1 (ovarian carcinoma) MCF-7 (breast cells leads to rapid robust induction...
Hormone-activated ERs (ERalpha and ERbeta) bind with high affinity to specific DNA sequences, estrogen response elements (EREs), located within the regulatory regions of target genes. Once considered function solely as receptor tethers, there is an increasing amount recent evidence suggest that sequence ERE can influence activity. In this study, we have performed a systematic analysis role different EREs in ER pharmacology. Specifically, by measuring activity on vitellogenin A2, complement 3...
The human estrogen receptor α (ERα) and the recently identified ERβ share a high degree of amino acid homology; however, there are significant differences in regions these receptors that would be expected to influence transcriptional activity. Consequently, we compared mechanism(s) by which regulate target gene transcription, evaluated cellular consequences coexpression both ER subtypes. Previously, it has been determined ERα contains two distinct activation domains, ERα-AF-1 ERα-AF-2, whose...
SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. necessary not sufficient development clinical disease. Currently, there are no approved pre- or post-exposure prophylactic medical countermeasures. Clinical data suggest that famotidine may mitigate disease, both mechanism action rationale dose selection remain obscure. We have investigated several plausible hypotheses activity including antiviral host-mediated...
The coronavirus disease 2019 (COVID-19) pandemic has affected the care of all patients around world. International League Against Epilepsy (ILAE) COVID-19 and Telemedicine Task Forces examined, through surveys to people with epilepsy (PWE), caregivers, health professionals, how well-being, care, services for PWE. ILAE included a link on their website whereby PWE and/or caregivers could fill out survey (in 11 languages) about impact pandemic, including access mental health, 6-item Kessler...
Concern that some chemicals in our environment may affect human health by disrupting normal endocrine function has prompted research on interactions of environmental contaminants with steroid hormone receptors. We compared the activity 2,2-bis-(p-hydroxyphenyl)-1,1,1-trichloroethane (HPTE), an estrogenic metabolite organochlorine pesticide methoxychlor, at estrogen receptor alpha (ERalpha) and beta (ERbeta). Human hepatoma cells (HepG2) were transiently transfected either or rat ERalpha...
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor with constitutive activities and those induced by xenobiotic ligands, such as 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD). One unexplained cellular role for the AHR its ability to promote cell cycle progression in absence of exogenous whereas treatment ligands induces arrest. Within cycle, from G<sub>1</sub> S phase controlled sequential phosphorylation retinoblastoma protein (RB1) cyclin D–cyclin-dependent...
The current statistics associated with breast cancer continue to show a relatively high recurrence rate together poor survival for aggressive metastatic disease. These findings reflect, in part, the pharmaceutical intractability of processes involved process and highlight need identify additional drug targets treatment late-stage In study, we report that ligand activation aryl-hydrocarbon receptor (AhR) inhibits multiple aspects panel cell lines represent major subtypes. Specifically, it was...
Most of the currently available information on transcriptional activities endocrine-disrupting chemicals (xenoestrogens) through estrogen receptors α (ERα) and β (ERβ) has been derived from transactivation studies synthetic estrogen-responsive reporters. Thus, ability xenoestrogen-liganded ERs to regulate endogenous gene expression not well characterized. Here, we have evaluated xenoestrogens ERα ERβ vitellogenin A2 estrogen-response element (ERE) human pS2, lactoferrin, complement 3...
The biological actions of estrogen are manifest through two genetically distinct receptors (ER alpha and ER beta) that display nonidentical expression patterns in target tissues. phenotypic alterations response to estrogens mice disrupted for either or both these not identical, suggesting each subtype plays a unique role ER-action. However, the lack subtype-specific agonists antagonists has made it difficult define processes regulated by and/or beta. Previously, we have reported...
Abstract The majority of ovarian cancers over‐express the estrogen receptor (ERα) and grow in response to estrogens. We previously demonstrated that ER induction chemokine CXCL12 (stromal cell‐derived factor‐1) is required for estradiol (E2)‐stimulated proliferation human carcinoma cells. In current study, we report known “endocrine disrupting chemicals” (EDCs) display mitogenic activities cancer cells via their ability activate upregulate expression. Notably, EDCs genistein, bisphenol A...
Hormone-activated ERs (ERα and ERβ) bind with high affinity to specific DNA sequences, estrogen response elements (EREs), located within the regulatory regions of target genes. Once considered function solely as receptor tethers, there is an increasing amount recent evidence suggest that sequence ERE can influence activity. In this study, we have performed a systematic analysis role different EREs in ER pharmacology. Specifically, by measuring activity on vitellogenin A2, complement 3 gene,...
The availability of multiple teleost (bony fish) genomes is providing unprecedented opportunities to understand the diversity and function gene duplication events using comparative genomics. Here we describe cloning functional characterization two novel vitamin D receptor (VDR) paralogs from freshwater medaka (Oryzias latipes). VDR sequences were identified through mining genome database in which organization structure was determined. Two distinct genes mapped defined loci. Each sequence...
Abstract SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. Currently, there are no pre- or post-exposure prophylactic medical countermeasures. Clinical data suggest that famotidine may mitigate disease, both mechanism action rationale dose selection remain obscure. We explore several plausible avenues activity including antiviral host-mediated actions. propose the principal involves on-target histamine receptor H 2...