A5015224335- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Soil and Water Nutrient Dynamics
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Mast cells and histamine
- Hydrology and Watershed Management Studies
- Virology and Viral Diseases
- COVID-19 Clinical Research Studies
- Microbial Inactivation Methods
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Transgenic Plants and Applications
- Immunotherapy and Immune Responses
- Pharmacological Receptor Mechanisms and Effects
- vaccines and immunoinformatics approaches
- DNA Repair Mechanisms
- Viral Infections and Vectors
- DNA and Nucleic Acid Chemistry
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Genetics and Reproduction
- Intramuscular injections and effects
- Heparin-Induced Thrombocytopenia and Thrombosis
Malone University
2016-2021
Icahn School of Medicine at Mount Sinai
2021
Tisch Cancer Institute
2021
Beloit College
2021
University of Manchester
2020
Institute of Science and Technology
2020
Alchem Laboratories (United States)
2020
University of Iowa
1999-2018
Harvard University
2016
University Hospital of Bern
2004
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein was readily detected all cases, no special delivery system required these effects. The extent of from both the constructs comparable to that obtained fibroblasts transfected vitro under optimal conditions. In situ cytochemical staining activity localized cells following injection vector. After luciferase...
We have developed an efficient and reproducible method for RNA transfection, using a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA), incorporated into liposome (lipofectin). Transfection of 10 ng to 5 micrograms Photinus pyralis luciferase mRNA synthesized in vitro NIH 3T3 mouse cells yields linear response activity. The procedure can be used efficiently transfect human, rat, mouse, Xenopus, Drosophila cells. Using the RNA/lipofectin...
The liver is an attractive target tissue for gene therapy. Current approaches hepatic delivery include retroviral and adenoviral vectors, liposome/DNA, peptide/DNA complexes. This study describes a technique direct injection of DNA into that led to significant expression. Gene expression was characterized in both rats cats following plasmid encoding several different proteins. Luciferase activity measured after the luciferase (pCMVL), β-galactosidase (β-Gal) evaluated situ using Lac Z...
The Root Zone Water Quality Model (RZWQM2) has been used widely for simulating agricultural management effects on crop production and soil water quality. Although it is a one-dimensional model, many desirable features the modeling community. This article outlines principles of calibrating model component by with one or more datasets validating independent datasets. Users should consult RZWQM2 user manual distributed along detailed protocol how to calibrate provided in book chapter. Two case...
SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. necessary not sufficient development clinical disease. Currently, there are no approved pre- or post-exposure prophylactic medical countermeasures. Clinical data suggest that famotidine may mitigate disease, both mechanism action rationale dose selection remain obscure. We have investigated several plausible hypotheses activity including antiviral host-mediated...
Naked DNA injection with electropermeabilization (EP) is a promising method for nucleic acid vaccination (NAV) and in vivo gene therapy. Skin an ideal target NAV due to ease of administration the accessibility large numbers antigen-presenting cells within tissue. This study demonstrates that skin EP may be used increase transgene expression up average 83-fold relative naked (50 μg per dose, P < 0.005). Transfected were principally located dermis included adipocytes, fibroblasts, endothelial...
Planting winter cover crops into corn-soybean rotations is a potential approach for reducing subsurface drainage and nitrate-nitrogen (NO3-N) loss. However, the long-term impact of this practice needs investigation. We evaluated RZWQM2 model against comprehensive field data (2005-2009) in Iowa used to study (1970-2009) hydrologic nitrogen cycling effects crop within rotation. The calibrated satisfactorily simulated yield, biomass, N uptake with percent error (PE) 15% relative root mean...
<h2>Abstract</h2> Preclinical in vivo rodent, porcine, and primate experiments aimed at enhancing nonviral transgene delivery to skin have been performed. These investigations identified a compound (aurintricarboxylic acid or ATA) that enhances transfection activity of "naked" plasmid pulsed electrical fields (electroporation EP) synergistically boosts expression an average 115-fold more than observed with free DNA (P < 0.00009). When is intradermally injected without ATA, the transfected...
A panel of lipidic tetraalkylammonium chlorides has been prepared and screened in studies both lipid hydration vivo mouse transfection. The effect cationic structure on liposome surface was determined using differential scanning calorimetry. Increases headgroup steric bulk the inclusion cis-unsaturation hydrophobic domain led to greater hydration, indicative a decrease polar associations. Cationic lipids containing hydrogen-bonding functionality exhibited corresponding observed an increase...
The critical physiological functions of the liver make hepatocytes important targets for therapeutic gene delivery. This study reports significant expression following direct injection plasmid DNA into livers rats and cats. Transfection was characterized using luciferase Lac Z from plasmids with cytomegalovirus immediate early promoter/enhancer (CMV IE) or Rous sarcoma virus long terminal repeat (RSV LTR). Dexamethasone treatment enhanced prolonged transfected expression, possibly by...
Drainage water management (DWM) has been proposed as a solution to reduce losses of nitrate (NO3) from subsurface drainage systems in the midwestern U.S.; however, tests DWM efficacy have only performed over short time periods and at limited number sites. To fill this gap, RZWQM-DSSAT hybrid model, previously evaluated for subsurface-drained agricultural system Iowa, was used simulate both conventional (CVD) 25 years historical weather 48 locations across U.S. Model simulations were...
Background: In 80% of patients, COVID-19 presents as mild disease1,2. 20% cases develop severe (13%) or critical (6%) illness. More forms present clinical acute respiratory syndrome, but include a T-predominant lymphopenia3, high circulating levels proinflammatory cytokines and chemokines, accumulation neutrophils macrophages in lungs, immune dysregulation including immunosuppression4. Methods: All major SARS-CoV-2 proteins were characterized using an amino acid residue variation analysis...
Background: In 80% of patients, COVID-19 presents as mild disease. 20% cases develop severe (13%) or critical (6%) illness. More forms present clinical acute respiratory syndrome, T-predominant lymphopenia, high circulating levels proinflammatory cytokines and chemokines, accumulation macrophages neutrophils in lungs, immune dysregulation including immunosuppression. Methods: All major SARS-CoV-2 proteins were characterized using an amino acid residue variation analysis method. Results...
Cationic liposome transfection reagents are useful for transferring polynucleotides into cells, and have been proposed human pulmonary gene therapy. The effect of adding cholesterol to cationic lipid preparations has tested by first formulating the N-[1-(2,3-dioleoyloxy)propyl-N-[1-(2-hydroxy)ethyl]-N,N-dimethyl ammonium iodide (DORI) with varying amounts dioleoylphosphatidylethanolamine (DOPE) cholesterol. Cholesterol was found enhance lipid-mediated in both respiratory epithelial cells...
Planting winter cover crops such as rye (Secale cereale L.) after corn and soybean harvest is one of the more promising practices to reduce nitrate loss streams from tile drainage systems without negatively affecting production. Because availability replicated tile-drained field data limited because use has only been tested over a few years with environmental management conditions, estimating impacts under range expected conditions difficult. If properly against observed data, models can...
Abstract SARS-CoV-2 infection is required for COVID-19, but many signs and symptoms of COVID-19 differ from common acute viral diseases. Currently, there are no pre- or post-exposure prophylactic medical countermeasures. Clinical data suggest that famotidine may mitigate disease, both mechanism action rationale dose selection remain obscure. We explore several plausible avenues activity including antiviral host-mediated actions. propose the principal involves on-target histamine receptor H 2...
Initiation of meiotic recombination in the yeast Saccharomyces cerevisiae requires at least 10 gene products. The initiation event creates double-strand breaks, which are then processed by other enzymes. A variety classical observations, such as existence nodules, have suggested that proteins catalyzing form a complex. lines evidence indicate Rad50p, Mre11p, and Xrs2p interact, genetic data suggesting interactions between Rec102p Rec104p been reported. It has recently shown Spo11p...
Zika virus was previously considered to cause only a benign infection in humans. Studies of recent outbreaks the Pacific, South America, Mexico and Caribbean have associated with severe neuropathology. Viral evolution may be one factor contributing an apparent change disease as it spread from Southeast Asia across Pacific Americas. To address this possibility, we employed computational tools compare phylogeny, geography, immunology RNA structure isolates Africa, Asia, In doing so, contrast...