A5081600211- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Amino Acid Enzymes and Metabolism
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Studies on Chitinases and Chitosanases
- Polyamine Metabolism and Applications
- Protein Structure and Dynamics
- Plant responses to elevated CO2
- Carbohydrate Chemistry and Synthesis
- Catalysis for Biomass Conversion
- Polysaccharides and Plant Cell Walls
- Coenzyme Q10 studies and effects
- Medicinal Plant Pharmacodynamics Research
- Biocrusts and Microbial Ecology
- Cassava research and cyanide
- Pancreatic function and diabetes
- Fungal and yeast genetics research
- Porphyrin Metabolism and Disorders
- Cancer Research and Treatments
- Nanocomposite Films for Food Packaging
- Steroid Chemistry and Biochemistry
- Ginseng Biological Effects and Applications
Shandong University of Technology
2015-2025
Chinese Academy of Sciences
2012-2023
Northwest Institute of Plateau Biology
2023
Xinjiang Institute of Ecology and Geography
2023
Tianjin University of Science and Technology
2019
MIT University
2019
RMIT University
2019
Tianjin Institute of Industrial Biotechnology
2012-2014
University of Chinese Academy of Sciences
2013
State Key Laboratory of Microbial Technology
2008
Abstract Heme is an iron-containing porphyrin compound widely used in the fields of healthcare, food, and medicine. Compared to animal blood extraction, it more advantageous develop a microbial cell factory produce heme. However, heme biosynthesis microorganisms tightly regulated, its accumulation highly cytotoxic. The current review describes biosynthetic pathway free heme, fermentation production using different engineered bacteria constructed by metabolic engineering, strategies for...
Surfactin is widely used in the petroleum extraction, cosmetics, biopharmaceuticals and agriculture industries. It possesses antibacterial antiviral activities can reduce interfacial tension. Bacillus are commonly as production chassis, but wild-type subtilis 168 cannot synthesise surfactin. In this study, phosphopantetheinyl transferase (PPTase) gene sfp* (with a T base removed) was overexpressed enzyme activity restored, enabling B. to surfactin with yield of 747.5 ± 6.5 mg/L. Knocking out...
meso-Diaminopimelate dehydrogenase (meso-DAPDH) is an NADP(+)-dependent enzyme which catalyzes the reversible oxidative deamination on d-configuration of meso-2,6-diaminopimelate to produce l-2-amino-6-oxopimelate. In this study, gene encoding a meso-diaminopimelate from Symbiobacterium thermophilum was cloned and expressed in Escherichia coli. addition native substrate meso-2,6-diaminopimelate, purified also showed activity toward d-alanine, d-valine, d-lysine. This catalyzed reductive...
Pro-Xylane is a biologically active C-glycoside compound in cosmetic products. The conventional method involves converting d-xylose from beech trees to 1-C-(β-d-xylopyranosyl)-acetone and reducing it with sodium borohydride. This produced borate salts as byproducts, requiring tedious removal process. In addition, (S)-Pro-Xylane shows superior biological activity compared (R)-Pro-Xylane. Hence, focus on the second step of reduction reaction, carbonyl reductase (CR) Candida orthopsilosis Co...
ABSTRACT In order to enlarge the substrate binding pocket of meso -diaminopimelate dehydrogenase from Symbiobacterium thermophilum accommodate larger 2-keto acids, four amino acid residues (Phe146, Thr171, Arg181, and His227) were targeted for site saturation mutagenesis. Among all mutants, single mutant H227V had a specific activity 2.39 ± 0.06 U · mg −1 , which was 35.1-fold enhancement over wild-type enzyme.
Divergent evolution exists in the meso-diaminopimelate dehydrogenase (meso-DAPDH) family. Taking meso-DAPDH from Corynebacterium glutamicium ATCC13032 (CgDAPDH) and Symbiobacterium thermophilum IAM14863 (StDAPDH) as examples, two subtypes differ terms of their amination ability toward 2-keto acids. The significant structural difference between these is an indel loop containing 16 amino acid residues, which CgDAPDH. However, function has still not been reported to date. Herein, mutagenesis,...
ABSTRACT meso -Diaminopimelate dehydrogenase ( -DAPDH) from Symbiobacterium thermophilum (StDAPDH) is the first member of -DAPDH family known to catalyze asymmetric reductive amination 2-keto acids produce d -amino acids. It important understand catalytic mechanisms StDAPDH and other enzymes in this family. In study, based on an evolutionary analysis examination activity, can be divided into two types. Type I showed highly preferable activity toward -diaminopimelate -DAP), type II exhibited...
A meso-diaminopimelate dehydrogenase (DAPDH) from Clostridium tetani E88 (CtDAPDH) was found to have low activity toward the D-amino acids other than its native substrate. Site-directed mutagenesis similar that carried out on residues mutated by Vedha-Peters et al. resulted in a mutant enzyme with highly improved catalytic ability for synthesis of acids. The crystal structures CtDAPDH apo form and complex (meso-DAP), D-leucine (D-leu), 4-methyl-2-oxopentanoic acid (MOPA) were solved....
Abstract Chitin deacetylase (CDA) can hydrolyse the acetamido group of chitin polymers to produce chitosans, which are used in various fields including biomedical and pharmaceutical industries, food production, agriculture, water treatment. CDA represents a more environmentally-friendly easier control alternative chemical methods currently utilised chitosans from chitin; however, majority identified CDAs display activity toward low-molecular-weight oligomers essentially inactive polymeric or...
The enzymatic synthesis of 2-furylhydroxymethylketone from furfural and formaldehyde was developed on a preparative scale, which provides promising alternative for the low-cost green industrial production SMIA.
meso-Diaminopimelate dehydrogenase (meso-DAPDH) is a good candidate for one-step synthesis of d-amino acid from 2-keto acids. Our previous research revealed the classification meso-DAPDH family and showed that type II meso-DAPDH, such as Symbiobacterium thermophilum (StDAPDH), could catalyze reductive amination. In this article, seven residues StDAPDH, which are highly conserved in each subfamily but different between two subfamilies, were targeted to explore relationships structure...
Graphical Abstract GRAPHICAL ABSTRACT
The mitochondrial citrate transporter (MCT) plays an important role in efflux from the mitochondria eukaryotes, and hence provides a direct correlation between carbohydrate metabolism lipid synthesis. Our previous studies on transporters confirmed presence of two MCTs (TCT CT) oleaginous Mucor circinelloides WJ11 associated with high accumulation. However, molecular mechanism by MCT M. is still unclear. To study transport CT, gene was expressed Escherichia coli , its product purified....