A5041146312- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Electrohydrodynamics and Fluid Dynamics
- Extracellular vesicles in disease
- Graphene and Nanomaterials Applications
- Biosensors and Analytical Detection
- Gold and Silver Nanoparticles Synthesis and Applications
- 3D Printing in Biomedical Research
- Nanoparticles: synthesis and applications
- Nanoparticle-Based Drug Delivery
- Nanopore and Nanochannel Transport Studies
- Cancer Genomics and Diagnostics
- Neuroscience and Neural Engineering
- Thermal properties of materials
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- Advanced Nanomaterials in Catalysis
- Polymer Surface Interaction Studies
- Advanced Glycation End Products research
- AI in cancer detection
- Nanowire Synthesis and Applications
- Retinal Imaging and Analysis
- Nanofabrication and Lithography Techniques
UNSW Sydney
2022
National University of Singapore
2018-2021
The University of Queensland
2013-2020
Brisbane School of Theology
2019-2020
CeNTI (Portugal)
2019
Personalis (United States)
2019
University of Glasgow
2011-2012
Kalasalingam Academy of Research and Education
2009-2010
Exosomes are vesicles which have garnered interest due to their diagnostic and therapeutic potential. Isolation of pure yields exosomes from complex biological fluids whilst preserving physical characteristics is critical for downstream applications. In this study, we use 100 nm-liposomes 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) cholesterol as a model system assess the effect exosome isolation protocols on vesicle recovery size distribution using single-particle analysis method. We...
Exosomes show promise as noninvasive biomarkers for cancer, but their effective capture and specific detection is a significant challenge. Herein, we report multiplexed microfluidic device highly of multiple exosome targets using tunable alternating current electrohydrodynamic (ac-EHD) methodology, referred to nanoshearing. In our system, electrical body forces generated by ac-EHD act within nanometers an electrode surface (i.e., the layer) generate nanoscaled fluid flow that enhances...
Abstract Tumor-derived exosomes possess significant clinical relevance due to their unique composition of genetic and protein material that is representative the parent tumor. Specific isolation as well identification proportions these clinically relevant (CREs) from biological samples could help better understand significance cancer biomarkers. Herein, we present a simple approach for quantification proportion CREs within bulk exosome population isolated patient serum. This can potentially...
A rapid and simple approach is presented to address two critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption reducing assay time. The demonstrated involves rationally designed fluorophore-integrated gold/silver nanoshells SERS nanotags utilizes alternative current electrohydrodynamic (ac-EHD)-induced nanoscaled surface shear forces enhance the capture kinetics. performance was validated in comparison with hydrodynamic...
Cancer diagnosis and patient monitoring require sensitive simultaneous measurement of multiple cancer biomarkers considering that single biomarker analysis present inadequate information on the underlying biological transformations. Thus, development selective assays for detection might improve clinical expedite treatment process. Herein, a microfluidic platform rapid, sensitive, parallel cancer‐specific protein from complex samples is presented. This approach utilizes alternating current...
Background: micro-RNAs (miRNAs) are stable, small, non-coding RNAs enriched in exosomes. Their variation levels according to different disease etiologies have made them a promising diagnostic biomarker for neurodegenerative diseases such as Alzheimer’s (AD). Altered expression of miR-320a, miR-328-3p, and miR-204-5p been reported AD frontotemporal dementia (FTD). Objective: To determine their reliability, we aimed examine the three exosomal miRNAs isolated from cerebrospinal fluid (CSF)...
Early diagnosis of disease requires highly specific measurement molecular biomarkers from femto to pico-molar concentrations in complex biological (e.g., serum, blood, etc.) samples provide clinically useful information. While reaching this detection limit is challenging itself, these contain numerous other non-target molecules, most which have a tendency adhere solid surfaces via nonspecific interactions. Herein, we present an entirely new methodology physically displace nonspecifically...
We report a tunable alternating current electro-hydrodynamic (ac-EHD) force which drives lateral fluid motion within few nanometers of an electrode surface. Because the magnitude this shear can be tuned externally (e.g., via application ac electric field), it provides new capability to physically displace weakly (nonspecifically) bound cellular analytes. To demonstrate utility nanoshearing phenomenon, we present data on purpose-built microfluidic devices that employ ac-EHD remove nonspecific...
Microfluidic flow based multiplexed devices have gained significant promise in detecting biomarkers complex biological samples. However, to fully exploit their use bioanalysis, issues such as (i) low sensitivity and (ii) high levels of nonspecific adsorption non-target species be overcome. Herein, we describe a new device for the sensitive detection multiple protein serum by using an alternating current (ac) electrohydrodynamics (ac-EHD) induced surface shear forces phenomenon referred...
Nanometric movements of the substrate on which endothelial cells are growing, driven by periodic sinusoidal vibration from 1 Hz to 50 applied piezo actuators, upregulate endothelin-1 and Kruppel-like factor 2 expression, increase cell adhesion. These in z (vertical) axis ranges 5 nm similar vertical extent protrusions themselves already reported literature. White noise vibrations do not produce these effects. Vibrational sweeps, if suitably confined within a narrow frequency range,...
Profiling tumor cells present in blood based on multiple tumor-relevant markers can identify specific subpopulations of and provide extensive information the status. Herein we a simple approach for analysis combination alternating current electrohydrodynamic (ac-EHD)-induced fluid flow capture, release, subsequent recapture from biological fluids. Initially, cell isolation was achieved using an epithelial adhesion molecule (EpCAM) antibody-modified ac-EHD device. Following this, captured...
Abstract We report a new tuneable alternating current (ac) electrohydrodynamics (ac‐EHD) force referred to as “nanoshearing” which involves fluid flow generated within few nanometers of an electrode surface. This can be externally tuned via manipulating the applied ac‐EHD field strength. The ability manipulate induced forces and concomitant micromixing enhance transport capture domain channel (e.g., analytes hence increase target–sensor interactions). also provides capability preferentially...