A5101874183- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- SARS-CoV-2 and COVID-19 Research
- Bacterial Genetics and Biotechnology
- COVID-19 Clinical Research Studies
- Advanced Data and IoT Technologies
- Technology and Security Systems
- Virus-based gene therapy research
- PARP inhibition in cancer therapy
- Telecommunications and Broadcasting Technologies
- Sinusitis and nasal conditions
- Sympathectomy and Hyperhidrosis Treatments
- Alzheimer's disease research and treatments
- Aluminum toxicity and tolerance in plants and animals
- Animal Disease Management and Epidemiology
- Pharmacological Effects of Natural Compounds
- Gout, Hyperuricemia, Uric Acid
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Photodynamic Therapy Research Studies
- IoT and Edge/Fog Computing
- Privacy-Preserving Technologies in Data
- Inflammasome and immune disorders
Hebei Agricultural University
2024
Beijing University of Chinese Medicine
2022
Shanghai Medical College of Fudan University
2010-2021
Fudan University
2017-2020
China United Network Communications Group (China)
2017-2020
Ningxia Medical University
2012
Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has rapidly become a global public health threat. efficacy of several repurposed drugs been evaluated in clinical trials. Among these drugs, second-generation antiandrogen agent, enzalutamide, was proposed because it reduces the expression transmembrane serine protease (TMPRSS2), key component mediating SARS-CoV-2-driven entry, prostate cancer cells. However, definitive...
Accumulating mutations in the SARS-CoV-2 Spike (S) protein can increase possibility of immune escape, challenging present COVID-19 prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 13G9, with high neutralizing potency blocking authentic virus display remarkable efficacy against B.1.351 virus. Surprisingly, structural analysis has revealed that 58G6 13G9 both recognize steric region S470-495 on RBD, overlapping...
PhoU homologs are one of the determinant factors in regulation persister formation and phosphate metabolism many bacterial species, however, functions exhibit species-specific characteristics. The pathogenesis Staphylococcus aureus is closely correlated with persisters virulence factors, yet, two homologs, PhoU1 PhoU2, S. aureus, unclear. In this study, single double deletion mutants phoU1 phoU2 were generated strain USA500 2395. ΔphoU1 or ΔphoU2 displayed a change virulence, compared to...
Abstract Background Staphylococcus epidermidis has emerged as one of the most important nosocomial pathogens, mainly because its ability to colonize implanted biomaterials by forming a biofilm. Extensive studies are focused on molecular mechanisms involved in biofilm formation. The LytSR two-component regulatory system regulates autolysis and formation aureus . However, role played S. remained unknown. Results In present study, we demonstrated that lytSR knock-out did not alter...
Biofilms play a crucial role in the pathogenicity of Staphylococcus epidermidis, while little is known about whether essential YycFG two-component signal transduction system (TCS) involved biofilm formation. We used antisense RNA (asRNA) to silence yycFG TCS order study its regulatory functions S. epidermidis. Strain 1457 expressing asRNA yycF exhibited significant delay (~4-5 h) entry log phase, which was partially complemented by overexpressing ssaA. The expression and yycG resulted 68 50%...
PhoU, a conserved protein that has been proposed to coordinate phosphate import, is negative regulator of drug tolerance in most bacteria. In Staphylococcus epidermidis, the role PhoU biofilm formation and not yet investigated. Two homologs genome S. epidermidis have identified by presence motif E(D)XXXD PhoU. We separately constructed ΔphoU1 ΔphoU2 mutants strain 1457. The mutant displayed growth retardation, weakened capacity, higher sensitivity H2O2, reduced multiple antibiotics. However,...
Staphylococcus epidermidis is frequently associated with biofilm-related infections. Biofilms drastically reduce the efficacy of conventional antibiotics and host immune system. In S. biofilm formation, a major role played by YycG/YycF two-component system, previous findings have indicated that inhibitors targeting cytoplasmic HATPase_c domain YycG kinase in exhibit bactericidal biofilm-killing activities. Therefore, we hypothesized monoclonal antibodies (mAbs) against extracellular (YycGex)...
The two-component system VraSR responds to the cell wall-active antibiotic stress in Staphylococcus epidermidis. To study its regulatory function biofilm formation, a vraSR deletion mutant (ΔvraSR) was constructed using S. epidermidis strain 1457 (SE1457) as parent strain. Compared SE1457, ΔvraSR showed impaired formation both vitro and vivo with higher ratio of dead cells within biofilm. Consistently, produced much less polysaccharide intercellular adhesin (PIA). also increased...
Abstract The SARS-CoV-2 infection is spreading rapidly worldwide. Efficacious antiviral therapeutics against urgently needed. Here, we discovered that protoporphyrin IX (PpIX) and verteporfin, two FDA-approved drugs, completely inhibited the cytopathic effect produced by at 1.25 μM 0.31 respectively, their EC50 values of reduction viral RNA were nanomolar concentrations. selectivity indices PpIX verteporfin 952.74 368.93, suggesting broad margin safety. Importantly, prevented in mice...
Purpose: Dispelling dampness, relieving turbidity and dredging collaterals decoction (DED), is a traditional Chinese medicine used in the treatment of hyperuricemia. We aimed to explore effect mechanism DED Methods: The effects (9.48, 4.74, 2.37 g/kg/d) on potassium oxonate (750 mg/kg/d)-induced hyperuricemia rats were evaluated by serum uric acid (UA), creatinine (CRE), blood urea nitrogen (BUN), renal pathological changes. Network pharmacology was identify effective components targets DED,...
Abstract Accumulating mutations in the SARS-CoV-2 Spike (S) protein can increase possibility of immune escape, challenging present COVID-19 prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 13G9, with high neutralizing potency blocking authentic virus displayed remarkable efficacy against B.1.351 virus. Each these mAbs combination one Ab recognizing non-competing epitope exhibited synergistic effect Surprisingly,...
To evaluate the photodynamic therapy (PDT) against multi-antibiotic-resistant Staphylococcus aureus (S. aureus) and epidermidis (S.epidermidis) obtained from patients with chronic rhinosinusitis (CRS).Forty-five CRS who had been given medical treatment but still needed endoscopic surgery were included in this study. The mucus middle meatus was collected these during surgery, followed by separation of S. drug sensitive test. strains which could form biofilm selected. Light emitting diode...