A5086130375- SARS-CoV-2 and COVID-19 Research
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Ferroptosis and cancer prognosis
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- vaccines and immunoinformatics approaches
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Mycobacterium research and diagnosis
- Air Quality and Health Impacts
- Viral gastroenteritis research and epidemiology
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Breast Cancer Treatment Studies
- Cancer-related Molecular Pathways
- SARS-CoV-2 detection and testing
- RNA Interference and Gene Delivery
- Hepatitis B Virus Studies
The Affiliated Yongchuan Hospital of Chongqing Medical University
2021-2025
Chongqing Medical University
2021-2025
Panzhihua Central Hospital
2018-2025
Jinan University
2023
Harbin Medical University
2016-2021
Third Affiliated Hospital of Harbin Medical University
2016-2021
Northeast Forestry University
2017
Zhejiang University
2013
The SARS-CoV-2 Omicron variant of concern (VoC) and its sublineages contain 31-36 mutations in spike escape neutralization by most therapeutic antibodies. In a pseudovirus assay, 66 the nearly 400 candidate therapeutics Coronavirus Immunotherapeutic Consortium (CoVIC) panel neutralize multiple sublineages. Among natural immunoglobulin Gs (IgGs), especially those receptor-binding domain (RBD)-2 epitope community, all neutralizers recognize bivalently, with both antigen-binding fragments...
Abstract Background Depression and sleep disturbances are associated with increased risks of various diseases mortality, but their impacts on mortality in cancer survivors remain unclear. The objective this study was to characterize the independent joint associations depressive symptoms outcomes survivors. Methods This population-based prospective cohort included aged ≥ 20 years ( n = 2947; weighted population, 21,003,811) from National Health Nutrition Examination Survey (NHANES) 2007–2018...
Chemokine (C-C motif) receptor 8 (CCR8) is a chemokine selectively expressed on tumor-infiltrating regulatory T cells (Tregs). Strong immunosuppression mediated by CCR8
Accumulating mutations in the SARS-CoV-2 Spike (S) protein can increase possibility of immune escape, challenging present COVID-19 prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 13G9, with high neutralizing potency blocking authentic virus display remarkable efficacy against B.1.351 virus. Surprisingly, structural analysis has revealed that 58G6 13G9 both recognize steric region S470-495 on RBD, overlapping...
Long noncoding RNAs (lncRNA) that are associated with immune checkpoints have not been identified, and the mechanism by which such lncRNAs might regulate expression of is unknown in human cancer. Immune checkpoint-associated (ICP-lncRNA) were identified validated via a comprehensive bioinformatic analysis The Cancer Genome Atlas data. These ICP-lncRNAs involved key response cell receptor signaling pathways. was upregulated correlated poor prognosis patients HLA complex P5 (HCP5) myocardial...
Adoptive immunotherapy using CAR-T cells is a promising curative treatment strategy for hematological malignancies. Current manufacture of clinical-grade based on lentiviral/retrovirus transfection T followed by anti-CD3/CD28 activation supplemented with IL-2 has been associated low efficiency and usually the use terminally differentiated effector cells. Thus, improving quality quantity are essential optimizing cell preparation. In our study, we focus role IL-21 in γ c cytokine conditions We...
Despite the growing knowledge of T cell responses in COVID-19 patients, there is a lack detailed characterizations for cell-antigen interactions and functions. Here, with predicted peptide library from SARS-CoV-2 S N proteins, we found that specific CD8+ were identified over 75% convalescent patients (15/20) an epitope protein, N361-369 (KTFPPTEPK), was most dominant our selected library. Importantly, discovered 2 N361-369-specific receptors (TCRs) high functional avidity independent CD8...
After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly efficiently generate effective NAbs SARS-CoV-2. Here, we integrated optimized a strategically screening method NAbs, which has enabled us obtain receptor-binding domain (RBD) specific within 6 days, followed by additional 9 days antibody production function analysis. Using this...
Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant treatment predicts survival. Studies have shown that patient-derived organoids can be cancer research drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples predict the of patients chemotherapy. We generated receiving When patient received chemotherapy, were treated with similar drugs, thereby...
T cell receptor (TCR)-engineered cells targeting neoantigens originated from mutations in KRAS gene have demonstrated promising outcomes clinical trials against solid tumors. However, the challenge lies developing tumor-specific TCRs that avoid cross-reactivity with self-antigens to minimize possibility of severe toxicities. Current research efforts been put towards strategies eliminate TCR off-target recognition. Naive repertoire was used for screening KRASG12D-reactive TCRs. Specific were...
<title>Abstract</title> <bold>Background</bold> Public neoantigens, including KRAS, TP53, and PIK3CA mutations, which are shared across various tumor types, have demonstrated significant immunogenicity offer great promise for cancer immunotherapy. Clinical trials targeting these public neoantigens yielded encouraging results, regression prolonged relapse-free survival. This study evaluates the HLA binding properties of T cell epitopes derived from to identify optimal target, further develops...
It remains unclear that trade-off between the benefits of regular physical activity (PA) and potentially harmful effects exposure to PM2.5 (particulate matter with aerodynamic diameter < 2.5 μm) in highly polluted regions. Therefore, we aimed investigate modification on associations PA volume intensity depressive symptoms glaucoma individuals or without symptoms. Data this study was obtained from China Health Retirement Longitudinal Survey (CHARLS) implemented during 2011 2020. were measured...
Adoptive immunotherapy with CMV-specific T cells (CMV-VSTs) has shown favorable efficacy and minimal adverse effects in clinical settings, serving as prophylaxis, preemptive and/or curative treatment for the restoration of immunity patients after allogeneic hematopoietic stem cell transplantation. The establishment a CMV-VST bank enables prompt use CMV-VSTs off-the-shelf therapeutics. can be generated through culture or immunomagnetic selection based on IFN-γ secretion multimer technology....
Background/Aims: The oncogenic role of linc00152 in pan-cancer is unclear. Methods: In this study, RNA-Seq 33 breast specimens was performed, and the expression validated by qPCR using 50 paired cancer tissues adjacent normal tissues. This result combined with revalidated Gene Expression Omnibus Cancer Genome Atlas data. Next, roles view prognosis, chemoresistance, genomic epigenetic regulation, including DNA methylation histone modification, potential biological function enrichment, basic...
Breast cancer patients at the same stage may show different clinical prognoses or therapeutic effects of systemic therapy. Differentially expressed genes breast were identified from GSE42568. Through survival, receiver operating characteristic (ROC) curve, random forest, GSVA and a Cox regression model analyses, that could be associated with survival time in cancer. The molecular mechanism was by enrichment, GSEA, methylation SNV analyses. Then, expression key gene verified TCGA dataset...
Abstract Hotspot driver mutations presented by human leukocyte antigens might be recognized anti-tumor T cells. Based on their advantages of tumor-specificity and immunogenicity, neoantigens derived from hotspot mutations, such as PIK3CA H1047L , may serve emerging targets for cancer immunotherapies. NetMHCpan V4.1 was utilized predicting neoepitopes mutation. Using in vitro stimulation, antigen-specific cells targeting the HLA-A*11:01-restricted mutation were isolated healthy donor-derived...
Abstract Endocrine resistance poses a significant clinical challenge for patients with hormone receptor-positive and human epithelial growth factor receptor 2-negative (HR + HER2−) breast cancer. Dysregulation of estrogen (ER) ERBB signaling pathways is implicated in development; however, the integration these remains unclear. While SMAD4 known to play diverse roles tumorigenesis, its involvement endocrine poorly understood. Here, we investigate role acquired HR HER2− Genome-wide CRISPR...
Facing the imminent need for vaccine candidates with cross-protection against globally circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutants, we present a conserved antigenic peptide RBD9.1 both T-cell and B-cell epitopes. can be recognized by disease 2019 (COVID-19) convalescent serum, particularly those high neutralizing potency. Immunization successfully induce production of receptor-binding domain (RBD)-specific antibodies in Balb/c mice. Importantly, immunized...
This study is aimed at exploring the potential mechanism of PPAR signaling pathway in breast cancer (BRCA) and constructing a novel prognostic-related risk model. We used various bioinformatics methods databases to complete our exploration this research. Based on TCGA database, we use multiple extension packages based R language for data conversion, processing, statistics. LASSO regression analysis establish model BRCA. And combined online websites, including GEPIA, ImmuCellAI, TIMER, GDSC,...
Currently, neutralizing antibody and vaccine strategies have been developed by targeting the SARS-CoV-2 strain identified during early phase of pandemic. Early studies showed that ability RBD or NTD antibodies to elicit infection enhancement in vivo is still controversial. There are growing concerns plasma from convalescent patients people receiving vaccines mediate ADE variants infections immune cells. Here, we constructed engineered double-mutant containing an mutation D614G spike (S)...
The evidence regarding the relationship between postnatal exposure of air pollution and child malnutrition indicators, as well corresponding urban-rural disparities, is limited, especially in low-pollution area low- middle-income countries (LMICs). Therefore, our aim was to contrast effect estimates varying ambient particulate matter (PM) on indicators urban rural areas Tibet, China.Six were evaluated this study, namely, Z-scores height for age (HFA), weight (WFA), (WFH), stunting,...
Abstract Neutralizing antibodies (Abs) have been considered as promising therapeutics for the prevention and treatment of pathogens. After outbreak COVID-19, potent neutralizing Abs to SARS-CoV-2 were promptly developed, a few those are being tested in clinical studies. However, there methodologies detailly reported on how rapidly efficiently generate interest. Here, we present strategically optimized method precisive screening monoclonal (mAbs), which enabled us identify receptor-binding...