A5052646795- Electron Spin Resonance Studies
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Nitric Oxide and Endothelin Effects
- Advanced MRI Techniques and Applications
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cancer, Lipids, and Metabolism
- Lanthanide and Transition Metal Complexes
- Cardiac electrophysiology and arrhythmias
- Free Radicals and Antioxidants
- Coenzyme Q10 studies and effects
- Neurological Disease Mechanisms and Treatments
- Ginkgo biloba and Cashew Applications
- Mitochondrial Function and Pathology
- Cardiac Ischemia and Reperfusion
- Organophosphorus compounds synthesis
- Cardiac Arrest and Resuscitation
- Phytochemicals and Antioxidant Activities
- Advanced NMR Techniques and Applications
- Metabolism and Genetic Disorders
- Biochemical effects in animals
- Muscle Physiology and Disorders
- Phytochemistry and Biological Activities
- Natural Compound Pharmacology Studies
Aix-Marseille Université
2012-2025
Institut de Chimie Radicalaire
2014-2023
Centre National de la Recherche Scientifique
2011-2022
Institut Français
2022
Institut des Sciences Moléculaires de Marseille
2006-2011
Laboratoire de Chimie Bactérienne
2009
Laboratoire de Réactivité de Surface
1997
Hôpital Lariboisière
1994
Centre de Résonance Magnétique Biologique et Médicale
1989-1991
University of Milan
1987
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide: A New Efficient Phosphorylated Nitrone for the in Vitro and Vivo Spin Trapping of Oxygen-Centered RadicalsClaudine Frejaville, Hakim Karoui, Beatrice Tuccio, Francois Le Moigne, Marcel Culcasi, Sylvia Pietri, Robert Lauricella, Paul TordoCite this: J. Med. Chem. 1995, 38, 2, 258–265Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January...
We have previously shown in cultured cerebellar granule neurons (Lafon-Cazal, M., Pietri, S., Culcasi, and Bockaert, J. (1993) Nature 364, 535-537) that upon N-methyl-D-aspartate stimulation, a nitric oxide synthase (NOS)-independent, arachidonic acid-dependent generation of superoxide free radicals (O2-.) is observed after lag time 10-15 min. Using the electron spin resonance trapping technique, we show stimulation produced more rapid burst O2-. L-arginine (L-Arg)-depleted neurons. These...
Abstract p53 exerts its tumor suppressor function mainly through transcriptional induction of target genes involved in several processes, including cell cycle checkpoints, apoptosis, and regulation redox status. antioxidant is dependent on activity proceeds by sequential proapoptotic targets. However, none the thus far renowned targets have proved able to abolish their own intracellular reactive oxygen species (ROS) accumulation caused deficiency, therefore pointing existence other prominent...
The present study examined whether dynamic cerebral autoregulation and blood-brain barrier function would become compromised as a result of exercise-induced oxidative-nitrosative stress. Eight healthy men were at rest after an incremental bout semi-recumbent cycling exercise to exhaustion. Changes in index determined during recovery from continuous recordings blood flow velocity the middle artery (MCAv) mean arterial pressure transiently induced hypotension. Electron paramagnetic resonance...
Chronic mountain sickness (CMS) is a maladaptation syndrome encountered at high altitude (HA) characterised by severe hypoxaemia that carries higher risk of stroke and migraine associated with increased morbidity mortality. We examined if exaggerated oxidative-inflammatory-nitrosative stress (OXINOS) corresponding decrease in vascular nitric oxide bioavailability patients CMS (CMS+) impaired cerebrovascular function adverse neurological outcome. Systemic OXINOS was markedly elevated CMS+...
5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), a newly synthesized spin trap, is used for the in vitro trapping of hydroxyl and superoxide radicals, though rates are close to those reported 5,5-dimethyl-1-pyrroline (DMPO), DEPMPO–superoxide adduct significantly more persistent than its DMPO analogue, difference which allows detection during reperfusion ischaemic isolated rat hearts.
Tumor protein 53-induced nuclear 1 (TP53INP1) is an antiproliferative and proapoptotic involved in cell stress response. To address its physiological roles colorectal cancer colitis, we generated tested the susceptibility of Trp53inp1-deficient mice to development tumors induced by injection carcinogen azoxymethane followed dextran sulfate sodium (DSS)-induced chronic colitis. showed increased incidence multiplicity compared those wild-type (WT) mice. Furthermore, acute colitis DSS treatment...
Photoinhibition and production of reactive oxygen species were studied in tobacco plants overexpressing the plastid terminal oxidase (PTOX). In high light, these was more susceptible to photoinhibition than wild-type plants. Also oxygen-evolving activity isolated thylakoid membranes from PTOX-overexpressing strongly inhibited light thylakoids contrast low PTOX overexpressor, protected against while wild type they significantly damaged. The superoxide hydroxyl radicals shown by EPR...
Mitochondrial free radical formation has been implicated as a potential mechanism underlying degenerative senescence, although human data are lacking. Therefore, the present study was designed to examine if resting and exercise-induced intramuscular radical-mediated lipid peroxidation is indeed increased across spectrum of sedentary aging. Biopsies were obtained from vastus lateralis in six young (26 + or - 6 yr) aged (71 males at rest after maximal knee extensor exercise. Aged tissue...
The broad range of applications cerium oxide (CeO2) nanoparticles (nano-CeO2) has attracted industrial interest, resulting in greater exposures to humans and environmental systems the coming years. Their health effects potential biological impacts need be determined for risk assessment. aims this study were gain insights into molecular mechanisms underlying genotoxic nano-CeO2 relation with their physicochemical properties. Primary human dermal fibroblasts exposed environmentally relevant...
The real‐time kinetics of the release ascorbyl free radicals in coronary perfusate from isolated rat hearts submitted to an ischemia/reperfusion sequence has been achieved by continuous‐flow ESR using high‐speed acquisition techniques. Enhanced detection was obtained addition dimethyl sulfoxide (Me 2 SO), a strong cation chelator and oxidizing agent. A device allowed direct monitoring radical and/or ascorbate leakage observation doublet ( H = 0.188 mT g 2.0054). results showed that occurred...
Aim The aim of this study was to examine if erythropoietin (EPO) has the potential act as a biological antioxidant and determine underlying mechanisms. Methods rate at which its recombinant form (rHuEPO) reacts with hydroxyl (HO˙), 2,2-diphenyl-1-picrylhydrazyl (DPPH˙) peroxyl (ROO˙) radicals evaluated in-vitro. relationship between erythopoietic oxidative–nitrosative stress response poikilocapneic hypoxia determined separately in-vivo by sampling arterial blood from eleven males in normoxia...
Tamanu oil from Calophyllum inophyllum L. has long been used in traditional medicine. Ethanol extraction was found the best strategy for recovering bioactive compounds resin part of oil, yielding two neutral and acidic resins fractions with high phenolics, flavonoids pyranocoumarins concentrations. A further cascade LPLC/HPLC separations allowed identifying fifteen metabolites, among them, calanolide D 12-oxocalanolide (both fraction) were first identified a natural source. All these...
Objective: Colorectal cancer is a major public health issue for which dietary factors such as red and processed meat consumption seem to play prominent role. Heme iron, present in important concentration those food products, was reported role colorectal promotion, animal studies. However, its initiation remains be established. Methods: Male Fischer 344 rats were given experimental diets (control diet, ferric citrate supplemented diet or hemin diet) two weeks before being initiated colon with...