A5011496545- Protein Hydrolysis and Bioactive Peptides
- Proteins in Food Systems
- Biochemical effects in animals
- Insect Utilization and Effects
- Phytase and its Applications
- Seed and Plant Biochemistry
- Moringa oleifera research and applications
- Food composition and properties
- Phytochemicals and Antioxidant Activities
- Meat and Animal Product Quality
- Natural Antidiabetic Agents Studies
- Consumer Attitudes and Food Labeling
- Food and Agricultural Sciences
- Food Chemistry and Fat Analysis
- Aquaculture Nutrition and Growth
- Enzyme Production and Characterization
- Biochemical Analysis and Sensing Techniques
- Hibiscus Plant Research Studies
- African Botany and Ecology Studies
- Antioxidant Activity and Oxidative Stress
- Antimicrobial Peptides and Activities
- Free Radicals and Antioxidants
- Morinda citrifolia extract uses
- Food Science and Nutritional Studies
- Sesame and Sesamin Research
University of Manitoba
2014-2024
Charles Sturt University
2013-2018
University of Winnipeg
2016
Wine Australia
2011-2014
In this study, the bambara protein isolate (BPI) was digested with three proteases (alcalase, trypsin and pepsin), to produce hydrolysates (BPHs). These were passed through ultrafiltration membranes obtain peptide fractions of different sizes (<1, 1-3, 3-5 5-10 kDa). The their investigated for antioxidant activities. membrane showed that peptides <3 kDa had significantly (p < 0.05) reduced surface hydrophobicity when compared >3 kDa. This is in agreement result obtained ferric reducing...
The aim of this work was to compare the antioxidant and angiotensin converting enzyme (ACE) inhibitory properties Moringa oleifera seed protein isolate (ISO) its enzymatic hydrolysates. ISO subjected (alcalase, pepsin trypsin) hydrolysis obtain alcalase isolate, trypsin hydrolysates (AIH, PIH, TIH). Amino acid composition similar for samples except that TIH had lower Sulphur-containing amino acids while PIH in tryptophan. All were tested through free radical scavenging abilities such as 2,2...
The effects of consuming hemp seed protein (HSP) as well its hydrolysate-derived bioactive peptide (HSP+) on blood pressure (BP) has not to our knowledge been investigated in humans. We aimed investigate how consumption HSP and hydrolysate modulates 24-hr systolic diastolic BP (24-hrSBP 24-hrDBP) plasma biomarkers compared casein. In a double-blind, randomized, cross-over design trial, 35 adults who had mild hypertension with SBP between 130 160 millimeters mercury (mmHg), DBP ≤110 mmHg were...
This report shows the antioxidant effects of a hemp seed meal protein hydrolysate (HMH) in spontaneously hypertensive rats (SHR). Defatted was hydrolyzed consecutively with pepsin and pancreatin to yield HMH, which incorporated into rat feed as source peptides. Young (8-week old) SHRs were divided three groups (8 rats/group) fed diets that contained 0.0%, 0.5% or 1.0% (w/w) HMH for eight weeks; half sacrificed blood collection. After 4-week washout period, remaining 20-week old an additional...
Abstract This work evaluated the functional properties of sesame protein fractions in order to determine their potential food applications. Sesame seed were prepared according solubility: water-soluble (albumin), salt-soluble (globulin), alkaline-soluble (glutelin) and ethanol-soluble (prolamin). Globulin was most abundant fraction, consisting 91% protein, followed by glutelin, albumin prolamin decreasing order. Non-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
Summary Defatted lentil seed flour proteins were separated into their constituent albumin (ALB), globulin (GLB) and glutelin (GLT) fractions followed by determination of structural functional properties. The GLB fraction demonstrated superior solubility (84%–100%) at acidic alkaline pH values when compared to the lower for ALB GLT. Amino acid composition analysis showed contents hydrophobic sulphur‐containing residues GLB. However, had highest in vitro protein digestibility, which may be due...
Abstract Legumes are rich sources of protein in human diet and their consumption has been associated with the prevention chronic diseases attributable to bioactive components. Pigeon pea ( Cajanus cajan ) is an underutilized legume relatively high content (~24%). Protein hydrolysates were prepared from isolate by enzymatic hydrolysis using pepsin pancreatin. Hydrolysates evaluated for amino acid composition, antioxidant properties, vitro vivo antihypertensive properties. The had hydrophobic...
The aim of this work was to determine the T2R4 bitter taste receptor-blocking ability enzymatic beef protein hydrolysates and identified peptide sequences. Beef hydrolyzed with each six commercial enzymes (alcalase, chymotrypsin, trypsin, pepsin, flavourzyme, thermoase). Electronic tongue measurements showed that had significantly ( p < 0.05) lower scores than quinine. Addition quinine led reduced bitterness intensity trypsin pepsin being most effective. HEK293T cells heterologously express...
An increased rate of high blood pressure has led to critical human hypertensive conditions in most nations. In the present study, bambara protein hydrolysates (BPHs) obtained using three different proteases (alcalase, trypsin and pepsin) their peptide fractions (molecular weight: 10, 5, 3 1 kDa) were investigated for antihypertensive antioxidant activities.Alcalase hydrolysate contained highest amount low molecular weight (LMW) peptides compared pepsin hydrolysates. LMW (<1 exhibited...
Proteins from tilapia frame and skin can potentially be precursors of antihypertensive peptides according to the result BIOPEP analyses. The aim was generate with inhibitory effects against angiotensin-converting enzyme (ACE) renin protein isolates (FPI SPI). most active hydrolysate then tested for blood pressure-lowering ability in spontaneously hypertensive rats (SHRs). Tilapia hydrolysates (FPHs SPHs) were respectively produced FPI SPI hydrolysis using pepsin, papain, or bromelain....
The aim of the study was to determine in vitro enzyme inhibition activities aqueous polyphenolic extracts nine popular Bangladeshi vegetables, namely ash gourd, bitter brinjal, Indian spinach, kangkong, okra, ridge snake and stem amaranth. Polyphenolic glycosides were major compounds present extracts. Inhibition α-amylase (up 100% at 1 mg/mL) stronger than α-glucosidase 70.78% 10 mg/mL). spinach extract strongest inhibitor pancreatic lipase activity (IC50 = 276.77 µg/mL), which significantly...
Isoelectric precipitation was used to produce protein isolates (>90% contents) from three edible vegetable leaves. Amaranth (ALI), eggplant (ELI) and fluted pumpkin (FLI) leaf were isolated study their conformational structures. Intrinsic fluorescence indicated a loose structural conformation for ELI at all the pH values, whereas FLI ALI had more compact structures 3.0 9.0. The surface hydrophobicity showed greater distribution of hydrophobic amino acid groups basic than acidic regions....
The aim of this work was to characterize the antioxidant properties some peptides present in bromelain mung bean meal protein hydrolysate (MMPH). MMPH subjected two rounds bioassay-guided reversed-phase HPLC separation followed by peptide identification most potent fractions using tandem mass spectrometry. Twelve peptides, namely, HC, CGN, LAN, CTN, LAF, CSGD, MMGW, QFAAD, ERF, EYW, FLQL, and QFAW were identified assayed for properties. CSGD (