Embelin (EMB) and Piperine (PIP) alkaloids are reported for ­antidiabetic, hypolipidemic, antioxidant, anti-cancer properties. However, simultaneous analytical methods scarce. A stability-indicating RP-HPLC method was developed with mobile phase MeOH: 0.1%TEA (pH 4.2 adjusted by OPA) (92:08 v/v ratio), CHEMSIL-ODS, C18 (4.6 × 250mm, 5 µm) column, 1.2 mL/min ­flowrate. Retention times were 3.2, 6.2 min PIP EMB respectively, at 289 nm, validated as per Q2 (R1) ICH guidelines. Linearity ranging...

Garuga pinnata a tree spotted in the Asian continent constitutes of constellation phytochemicals whole from which alcoholic extract leaf is abundant source. The namely Amentoflavone, Garuganin-1, Garuganin-3, Garuganin-4, and Garuganin-5 were considered for study as they have anti-Alzheimer's potential but biological target has not been reported. So, to identify scrutinized by employing silico methodologies molecular docking, dynamics simulation, ADMET prediction. Molecular docking revealed...

Difference spectrophotometric method was developed for the estimation of telmisartan in bulk drug and pharmaceutical formulations. Telmisartan exists two different forms acidic basic mediums that differ their UV spectra. spectrum, obtained by keeping 0.01 N NaOH reference cell HNO 3 sample cell, showed characteristic peaks at 295 nm 327 with positive negative absorbance respectively. between these maxima calculated to find out amplitude, which plotted against concentration. The found be...

Abstract: Background: Metformin (MET) is an oral antidiabetic agent falls chemically under the category of biguanides and it effectively utilized in management type 2 diabetes mellitus. It marketed tablet dosage form hence quality control assessment MET very much essential important. Objectives: The objectives present research investigation are to implement Box-Behnken Design (BBD) optimization validation Reverse Phase-High Performance Liquid Chromatographic (RP-HPLC) method for also...

Abstract Background Based on bioisosteric similarities with thiacetazone, a series of 7-chloro-4-aminoquinoline derivatives have been designed and synthesized. The target compounds were elucidated by NMR, mass, FTIR spectral data. All synthesized evaluated for their in vitro antitubercular activity against Mycobacterium tuberculosis H 37 Rv (MTB), human dermal fibroblast cell lines used to assess toxicity selected ligands. Results the showed inhibition MTB MIC 1.56–50 μM. Among tested...

Abstract Cardiovascular (CVS) drugs are medications whose primary effects experienced by the heart and blood vessels to treat cardiovascular disorders such as coronary artery disease, arrhythmias, high blood-pressure, failure stroke. Evaluation of drug-likeness is a qualitative research approach in drug design that examines drug-like properties any chemical compound used lead terms factors oral bioavailability. These ADME parameters can be widely discovery optimize needed convert candidates...

The current drug research techniques, combinatorial synthesis and high throughput screening, help in obtaining pre-evaluating thousands of compounds a short duration time.Among the selected compounds, best hit molecule becomes lead, likeness property helps to optimize selection.Lipinski's Rule five is most widely used filter pre-evaluation according Lipinski's with poor oral absorption low permeation has molecular weight >500, ClogP >5, hydrogen bond acceptor more than 10 donor 5.To evaluate...

Basavaraj Dinnimath, Mahesh Palled, Shradha Sutar, Tashildar, Utkarsh Chavan and Sampada Ganpule Author informationCitations Corresponding author. Correspondence: Dr. M Dinnimath Department of Pharmaceutical Chemistry, KLE College Pharmacy, Belagavi-590010, Karnataka, INDIA. Email: bmdinnimath@gmail.com Notes December 07, 2023; January 19, 2024; February 28, 2024. Copyright License information ©2024 Phcog.Net This is

Aim: The present study deals with development and validation of accurate, sensitive cost-effective RP-HPLC method for Loxoprofen sodium. Method: PRIMESIL C18 column (id 4.6 x 250 mm length) was used as stationary phase a mobile mixture methanol 0.05% OPA buffer in ratios 75:25 v/v. 20µl samples were injected at flow rate 1 ml/min. detector is G-13148 (DAD) detector, wavelength observed 225nm ambient temperature. Further, the developed validated per ICH guidelines. Results: method's linearity...

Antimicrobial resistance presents a significant threat to contemporary healthcare systems, making it increasingly difficult treat infections that were once easily managed. This study aims assess the efficacy of pyrazole benzamide derivatives (M5a–M5o), which synthesized for their antimicrobial and antifungal activities. These compounds tested against methicillin resistant staphylococcus aureus (MRSA), vancomycin (VRSA), mycobacterium tuberculosis H37Rv other species. The minimum inhibitory...

This study emphasizes the crucial role of Quality by Design (QbD) in developing pharmaceutical procedures, particularly risk assessment. It demonstrates how QbD principles were applied to create a precise and effective HPLC method for Silymarin Tablets, ensuring consistent quality within specified criteria. The optimized method, developed using Experiment approach, employs C18 column (150 mm x 4.6 mm, 5μm) with isocratic elution 95:25 ratio acetonitrile orthophosphoric acid buffer (pH 3)....

A simple, economical, fast, and precise reverse phase high performance liquid chromatographic method has been developed for the determination of rabiprazole in tablet dosage form. C 18 Hypersil (5 micron 25 cm×4.6 mm) column from Thermo, isocratic mode with mobile acetonitrile:phosphate buffer:methanol(50:40:10) The flow rate is 1.4 ml/min, effluent was monitored at 232 nm.

Cholate-based nano emulgel of 1,1-dimethylbiguanide can diminish the liver damage in NAFLD induced by high-fat dietHeba F. Salem, Mohammed M. Nafady, Adel A. Ali, Nermeen Khalil, Amani Elsisi

One of the most widely spreading diseases due to several lifestyle problems in 21st century is diabetes mellitus. The management mellitus very important and essential. Plants are natural reservoir many medicinal value added components help overcome chronic disorders including Herbal drugs prescribed treatment their good effectiveness, fewer side effects clinical experience relatively low costs. Screening antidiabetic therapeutics essential for effective Many researchers have worked on...

Following publication of the original article [1], authors reported an error in one authors’ names.

Tuberculosis is a disease that has been known from the earliest of recorded history. The development multidrug-resistant TB (Mtb) strains, treatment active more complicated than it used to be. Therefore, there an urgent need for novel anti-tuberculosis drugs, which will be against both drug-sensitive and drug-resistant Mtb strains. FtsZ (Filamental temperature-sensitive protein Z), tubulin homolog, most critical bacterial cell division. GTP dependent polymerization forms dynamic helical...

In the present research work a new simple, specific, precise and accurate Ultra-fast liquid chromatographic method was developed validated for estimation of Isoniazid in bulk marketed pharmaceutical dosage forms.Experimental Approach: The by using BDS Hypersil C-8 column (5µm, 250 mm x 4.6 mm) as stationary phase Milli Q water: Methanol (95:05%v/v) mobile phase.The flow rate 1 ml/min.The analysis performed at ambient temperature with UV detection 290nm.The retention time found to be 6.8...

The objectives of present research work was to develop simple, precise, accurate, specific, cost effective, validated UV-spectrophotometric method for quantification Agomelatin (AGM) in bulk and pharmaceutical tablets dosage form by Area Under Curve (AUC) Zero Order Spectroscopic (ZOS) methods. Both methods involve the use methanol: millipore water (50:50 %v/v) as solvent estimation. area between 212-237 nm used measure AUC first 229 absorbance second method. developed technique optimized...