A5008006400- biodegradable polymer synthesis and properties
- Biopolymer Synthesis and Applications
- Microplastics and Plastic Pollution
- Enzyme Catalysis and Immobilization
- Graphene and Nanomaterials Applications
- Lignin and Wood Chemistry
- Microbial Metabolic Engineering and Bioproduction
- Antimicrobial agents and applications
- Microbial bioremediation and biosurfactants
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Advanced Cellulose Research Studies
- Electrospun Nanofibers in Biomedical Applications
- Carbon dioxide utilization in catalysis
- Pickering emulsions and particle stabilization
- Polymer composites and self-healing
- Hydrogels: synthesis, properties, applications
- Micro and Nano Robotics
- Surfactants and Colloidal Systems
- Mass Spectrometry Techniques and Applications
- Characterization and Applications of Magnetic Nanoparticles
- RNA Interference and Gene Delivery
- Solar-Powered Water Purification Methods
- Metabolomics and Mass Spectrometry Studies
- Fungal and yeast genetics research
National Institute of Advanced Industrial Science and Technology
2018-2024
Fukui Prefectural University
2015-2022
Tokyo Institute of Technology
2012-2016
Abstract Sophorolipids (SLs) are promising glycolipid biosurfactants as they easily produced and functional. SLs from microorganisms comprised of mixtures multiple derivatives that have different structures properties, including well-known acidic lactonic SL (ASLs LSLs, respectively). In this study, we established a method for analyzing all in the products Starmerella bombicola , typical SL-producing yeast. Detailed component analyses S. were carried out using reversed-phase high-performance...
ABSTRACT In this study, we performed in vitro and vivo activity assays of polyhydroxyalkanoate (PHA) synthases (PhaCs) the presence phasin proteins (PhaPs), which revealed that PhaPs are activators PhaC derived from Aeromonas caviae (PhaC Ac ). assays, among three PhaCs tested, was significantly activated when were added at beginning polymerization (prepolymerization ), whereas prepolymerization Re (derived Ralstonia eutropha ) Da ( Delftia acidovorans showed reduced with PhaPs. The...
ε-Poly-l-lysine (ε-PL) is one of the few homopoly(amino-acid)s occurring in nature. ε-PL, which possesses multiple amino groups, highly soluble water, where it forms antimicrobial polycationic chain (PLn+). Although high water-solubility advantageous for use ε-PL as a food preservative, has limited applicability biopolymer plastic. Here, we report on preparation and availability water-insoluble complex formed with PLn+ an anionic surfactant, bis(2-ethylhexyl) sulfosuccinate (BEHS–, also...
Abstract Sixteen strains of basidiomycetous yeasts were evaluated for their capability to produce ergothioneine (EGT), an amino acid derivative with strong antioxidant activity. The cells cultured in either two synthetic media or yeast mold (YM) medium 72 h, after which cytosolic constituents extracted from the hot water. After analyzing extracts via liquid chromatography-mass spectrometry (LC-MS), we found that all produced varying amounts EGT. EGT-producing strains, including Ustilago...
The type I polyhydroxyalkanoate synthase from Cupriavidus necator was heterologously expressed in Escherichia coli with simultaneous overexpression of chaperone proteins. Compared to expression alone (14.55 mg liter(-1)), coexpression chaperones resulted the production larger total quantities enzyme, including a proportion soluble fraction. largest increase seen when GroEL/GroES system coexpressed, resulting approximately 6-fold-greater enzyme yields (82.37 liter(-1)) than absence...
Mannosylerythritol lipids (MELs), which are one of the representative sugar-based biosurfactants (BSs) produced by microorganisms, have attracted much attention in various fields sustainable development goals (SDGs) era. However, they inseparable mixtures with respect to chain length fatty acids. In this study, self-assembling properties and structure-activity relationship (SAR) studies recovery effects on damaged skin cells using chemically synthesized MELs were investigated. It was...
Bacteria produce polycationic homopoly(amino acid)s, which are characterized by isopeptide backbones. Although the biological significance of acid)s remains unclear, increasing attention has recently been focused on their potential use to achieve cellular internalization. Here, for first time, we provide direct evidence that two representative bacterial isopeptides, ε-poly-L-α-lysine (ε-PαL) and ε-oligo-L-β-lysine (ε-OβL), were internalized into mammalian cells cell-membrane penetration then...
We have developed a moldable and self-healable material from lignosulfonate by taking advantage of the ionic interactions between various cationic polyelectrolytes. Three complexes with different polyelectrolytes were prepared. The complex sodium (L-SO3Na) poly(diallyldimethylammonium chloride) (PDADMACl) was deemed promising based on its mechanical toughness in tensile tests (>3 MJ/m3). properties L-SO3Na/PDADMACl further characterized regard to effects composition, molecular weight...
Synthesis of three types purpose-designed mannosylerythritol lipid (MEL)-D analogues with decanoyl groups, β-GlcEL-D, α-GlcEL-D, and α-MEL-D, was accomplished utilizing our boron-mediated aglycon delivery (BMAD) methods. Their self-assembling properties, recovery effects on damaged skin cells, antibacterial activity were evaluated. It revealed, for the first time, that α-GlcEL-D α-MEL-D only generated giant vesicles, indicating slight differences in steric configuration an erythritol moiety...
A moldable material from a natural cationic polyelectrolyte, ε-poly-l-lysine (ε-PL), was prepared by mixing with two lignosulfonates reagent for research (L-SO3Na) and commercially available purified lignosulfonate (Pearllex NP). The obtained ε-PL/lignosulfonate complexes demonstrated the ability to be tuned rigid form, such as polystyrene or poly(methyl methacrylate), soft elastomer form silicone varying species composition. maximum toughness of complex (8.4 MJ/m3) superior that ε-PL...
A separation method for a biopolymer, ε-poly-L-lysine (εPL), with its colorimetric determination using yellow anionic dye, dipicrylamine anion (DPA(-)), is presented. The εPL-producing culture broth was mixed NaDPA solution to precipitate the εPL in polycationic form DPA(-) anion. dissolved into acetonitrile (AN). AN yellowish, and gave an absorption maximum at around 420 nm. Thus, can be assayed colorimetrically multiwell microtiter plate. By addition of...
We report a bio-based, soft, elastic, and tough material prepared from mixture of ε-poly-l-lysine (ε-PL) d-fructose. The obtained complex was insoluble in water, whereas its ingredients had high water solubility. This likely formed via Schiff base formation subsequent rearrangement reactions, that is, the Maillard reaction, because reaction occurred between reducing sugars cationic polyelectrolytes having primary secondary amino groups. progress investigated by proton nuclear magnetic...
We developed a method for the immobilization of multiple active enzymes, allowing production chiral products from nonchiral substrates with recycling expensive cofactors. Using rapid, two-step process under nondenaturing conditions, we could preserve enzyme activity by separating an scaffold attachment enzymes. The technique is applicable to wide range enzymes and will facilitate simple, cost-effective research industrial purposes. An (R)-specific poly(hydroxyalkanoate) synthase (PhaCRe...
The initial stage of in vitro polyhydroxyalkanoate (PHA) polymerization by PHA synthase from Ralstonia eutropha (PhaCRe) on a mica substrate water was observed using high-speed scanning atomic force microscopy (HS-AFM). Before polymerization, the adsorption-desorption cycle PhaCRe molecule real time. For approximately 30 s after addition monomer, no significant change substrate, but could be transformed into an active enzyme upon contact with monomer during this period. Subsequently,...
ABSTRACT Recombinant Ralstonia eutropha strain PHB − 4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. 61-3 (PhaC1 Ps ) synthesizes a PHA copolymer containing branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has carbon backbone identical to that of leucine. Mutant 1F2 was derived R. by chemical mutagenesis and shows higher levels 3H4MV production than does parent strain. In this study, understand mechanisms underlying...
Polyelectrolyte complexes composed of lignosulfonate and cationic polyelectrolytes were used as bio-based adhesives. Sodium (L-SO3Na), a wood-derived anionic polyelectrolyte, was combined with three different the adhesive strength resulting evaluated on various substrates. Higher observed polar substrates (stainless steel, aluminum, wood) compared to nonpolar substrate (polypropylene). Complexes L-SO3Na/poly(allylamine) L-SO3Na/ε-poly-l-lysine exhibited higher than other polyelectrolyte...