The study of yeast cell morphology requires consistent identification cycle phases based on bud size. A computer-based image processing algorithm is designed to automatically classify microscopic images cells in a microfluidic channel environment. were enhanced reduce background noise, and robust segmentation developed extract geometrical features including compactness, axis ratio, are then used for classification, the accuracy various machine-learning classifiers compared. linear support...

This is the first part of a two-part study on generation droplets at microfluidic T-junction operating in transition regime where confinement droplet creates large squeezing pressure that influences formation. In this regime, operation depends geometry intersection (height-to-width ratio, inlet width ratio), capillary number, flow and viscosity ratio two phases. Here paper I we presented our experimental observations through analysis high-speed videos formation process. Various parameters...

This is the second part of a two-part study on generation droplets at microfluidic T-junction operating in transition regime. In preceding paper [Phys. Rev. E 85, 016322 (2012)], we presented our experimental observations droplet formation and decomposed process into three sequential stages defined as lag, filling, necking stages. Here develop model that describes performance generators working squeezing to regimes where confinement dominates process. The incorporates detailed geometric...

A novel method is presented for on-chip temperature measurements using a poly(dimethylsiloxane) (PDMS) thin film dissolved with Rhodamine B dye. This sandwiched between two glass substrates (one of which 150 μm thick) and bonded to microchannel molded in PDMS substrate. Whole-chip (liquid substrate) can be obtained via fluorescent intensity visualization. For verification purposes, the was tested tapered subjected Joule heating, resulting axial gradients comparing well numerical simulations....

In this study, we present a microwave-based microfluidic mixer that allows rapid mixing within individual droplets efficiently. The designed microwave is coplanar design with small footprint, which fabricated on glass substrate and integrated chip. works essentially as resonator accumulates an intensive electromagnetic field into spiral capacitive gap (around 200 μm), provides sufficient energy to heat-up pass through the gap. This actuation induces nonuniform Marangoni stresses interface,...

This study extends our previous work on droplet generation in microfluidic T-junction generators to include dynamic interfacial tension effects created by the presence of surfactants. In Paper I [T. Glawdel, C. Elbuken, and L. Ren, Phys. Rev. E 85, 016322 (2012)], we presented experimental findings regarding formation process squeezing-to-transition regime, II 016323 (2012)] developed a theoretical model that describes performance without Here for two surfactants, one with small molecular...

Droplet-based microfluidics is an emerging high-throughput screening technology finding applications in a variety of areas such as life science research, drug discovery and material synthesis. In this paper we present cost-effective, scalable microwave system that can be integrated with microfluidic devices enabling remote, simultaneous sensing heating individual nanoliter-sized droplets generated microchannels. The key component electrically small resonator able to distinguish between...

Developing a microfluidic chip with multiple functions is highly demanded for practical applications, such as chemical analysis, diagnostics, particles synthesis and drug screening.

96-Well plate has been the traditional method used for screening drug compounds libraries potential bioactivity. Although this proven successful in testing dose-response analysis, microliter consumption of expensive reagents and hours reaction analysis time call innovative methods improvements. This work demonstrates a droplet microfluidic platform that to significantly reduce reagent shorten by utilizing nanoliter-sized droplets as replacement wells. is evaluated applying it screen inhibit...

We report a microwave-microfluidics integrated approach capable of detecting droplet at high-throughput and label-free sensing individual content without physical intrusion.

Soft robotics has gone through a decade of tremendous progress in advancing both fundamentals and technologies. It also seen wide range applications such as surgery assistance, handling delicate foods, wearable assistive systems driven by its soft nature that is more human friendly than traditional hard robotics. The rapid growth introduces many challenges, which vary with applications. Common challenges include the availability materials for realizing different functions precision speed...

ABSTRACT Free‐flow counterflow gradient focusing (FF‐CGF) is a promising tool for protein fractionation. This work establishes guidelines to construct an FF‐CGF system suitable preparative applications. Numerical and analytical computations are undertaken investigate design parameters, including device geometry, flow conditions, sample concentration, applied voltage. collective information serves as guide the fabrication of device, which, in this case, constructed from polymethyl...

This study presents a microfluidic system that incorporates electroosmotic pumps, concentration gradient generator and fish cell line (rainbow trout gill) to perform toxicity testing on cells seeded in the system. The consists of three mechanical components: (1) chip containing which creates linear distribution toxicant test chamber, (2) an (EO) pump controls flow rate operation chip, (3) indirect reservoirs connect two chips allowing for solution be pumped separately from solution....

The global COVID-19 pandemic caused by SARS-CoV-2 has resulted in an unprecedented economic and societal impact. Developing simple accurate testing methods for point-of-care (POC) diagnosis is crucial not only the control of COVID-19, but also better response to similar outbreaks future. In this work, we present a novel proof-of-concept microfluidic microwave sensing method POC virus. This relies on antibody immobilized sensor selectively capture concentrate antigen or virus buffer solution...

Escherichia coli (E. coli) O157:H7 (O157), one of the most common Shiga toxin-producing E. coli, can contaminate water systems causing severe illnesses often accompanied with diarrhea and sometimes life threatening. Frequent monitoring in is critical to protect public health. Most traditional methods for detection are slow responding outbreaks due need sample transportation from site lab, expensive equipment, highly trained personnel detection. This work presents a novel reagent-free method...

Rhodamine B based fluorescence thermometry is commonly used in microfluidics to measure fluid temperatures microchannels. Notable absorption of into PDMS channel walls, however, causes difficulties obtaining accurate temperature measurements due a steady increase the overall fluorescent signal. A simple and effective technique reported that removes signal from absorbed dye by means photobleaching with high intensity light source before taking images for analysis. The field at convergence hot...

A poly(dimethylsiloxane)(PDMS)/SU-8/quartz hybrid chip was developed and applied in the isoelectric focusing (IEF) of proteins with ultraviolet (UV) absorbance-based whole-channel imaging detection (UV-WCID). Each made three layers: a PDMS flat top substrate, bottom quartz substrate middle layer SU-8 photoresist. The serves two purposes: it contains microchannel used for IEF separation, acts as an optical slit that absorbs UV light below 300 nm improving sensitivity WCID. novel design...

When droplets enter a junction they sort to the channel with highest flow rate at that instant. Transport is regulated by discrete time-delayed feedback results in highly periodic behavior where specific patterns can continue cycle indefinitely. Between these ordered regimes are chaotic structures no pattern evident. Here we develop model describes droplet sorting under various asymmetries: branch geometry (length, cross-section), resistance and pressures. First, developed based on continuum...