A5053289851- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- Neuropeptides and Animal Physiology
- Corneal surgery and disorders
- PARP inhibition in cancer therapy
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Protein Kinase Regulation and GTPase Signaling
- Advanced Fluorescence Microscopy Techniques
- Analytical Chemistry and Sensors
- Hippo pathway signaling and YAP/TAZ
- Cancer, Stress, Anesthesia, and Immune Response
- Conducting polymers and applications
- Aortic Disease and Treatment Approaches
- Optical Coherence Tomography Applications
- Retinal Diseases and Treatments
- Cellular transport and secretion
- Molecular Sensors and Ion Detection
- Neuroscience and Neural Engineering
- Cancer-related gene regulation
Oregon Health & Science University
2018-2023
Portland State University
2017
University of Michigan
2002
Junctions between the endoplasmic reticulum (ER) and plasma membrane (PM) are specialized contacts ubiquitous in eukaryotic cells. Concentration of intracellular signaling machinery near ER-PM junctions allows these domains to serve critical roles lipid Ca2+ homeostasis. Subcellular compartmentalization protein kinase A (PKA) also regulates essential cellular functions, however, no specific association PKA junctional is known. Here, we show that brain neurons type I directed Kv2.1...
Electrical coupling, mediated by gap junctions, contributes to signal averaging, synchronization, and noise reduction in neuronal circuits. In addition, junctions may also provide alternative pathways. However, because they are small especially difficult image, often ignored large-scale 3D reconstructions. Here, we reconstruct between photoreceptors the mouse retina using serial blockface-scanning electron microscopy, focused ion beam-scanning confocal microscopy for junction protein Cx36....
Class A G-protein-coupled receptors (GPCRs) normally function as monomers, although evidence from heterologous expression systems suggests that they may sometimes form homodimers and/or heterodimers. This study aims to evaluate possible functional interplay of endogenous µ- and δ-opioid (MORs DORs) in mouse neurons. Detecting GPCR dimers native tissues, however, has been challenging. Previously, MORs DORs co-expressed transfected cells have reported heterodimers, their co-localization...
Yes-associated protein 1 (YAP1) is indispensable for the development of mutant KRAS-driven pancreatic ductal adenocarcinoma (PDAC). High YAP1 mRNA a prognostic marker worse overall survival in patient samples; however, regulatory mechanisms that mediate its overexpression are not well understood. genetic alterations rare PDAC, suggesting dysregulation likely due to events. HuR an RNA-binding whose inhibition impacts many cancer-associated pathways, including “conserved signature” as...
The ability of the visual system to relay meaningful information over a wide range lighting conditions is critical functional vision, and relies on mechanisms adaptation within retina that adjust sensitivity gain as ambient light changes. Photoreceptor synapses represent first stage image processing in system, thus activity-driven changes at this site are potentially powerful, yet under-studied means adaptation. To insight into these mechanisms, abundance distribution key synaptic proteins...
Abstract Class A G protein-coupled receptors (GPCRs) normally function as monomers, although evidence from heterologous expression systems suggests they may form homodimers and/or heterodimers. Detection of GPCR dimers in native tissues has however been challenging due to the lack suitable tools. μ- and δ-Opioid (MORs DORs) co-expressed transfected cells reported The co-localization MORs DORs neurons studied knock-in mice expressing genetically engineered fused fluorescent proteins. Here we...
Abstract Photorefractive keratectomy (PRK) is an alternative to LASIK and can cause intense acute pain that often not relieved by standard treatments. To assess potential therapeutics for this type of pain, appropriate preclinical models are needed. Herein we describe a rodent model PRK multi-faceted approach determine the therapeutic resveratrol, natural phytoestrogen, on tear production, corneal epithelium. Studies were conducted in male female Sprague-Dawley rats. Heptanol was applied one...
Abstract Alterations in DNA damage response (DDR) proteins are prevalent across cancers and present unique opportunities for the therapeutic induction of synthetic lethality (i.e., two gene deficiencies same pathway synergize to kill tumor cells). In pancreatic adenocarcinoma, patients harboring alterations DDR genes, such as homology-directed repair (HDR), comprise 10-20% that may benefit from recent FDA approval Olaparib, a poly (ADP-ribose) polymerase inhibitor disrupts non-homologous end...
Abstract Using serial blockface-scanning electron microscopy (SBF-SEM) and focused ion beam-scanning (FIB-SEM), combined with confocal for the gap junction protein Cx36, we reconstructed mouse photoreceptor terminals located junctions between them. An exuberant spray of fine telodendria extends from each cone pedicle (including blue cones) to contact 40-50 nearby rod spherules where Cx36 clusters were located, close mouth synaptic opening. There approximately 50 per 2-3 spherule. We unable...