A5100318155- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Diseases and Treatments
- Circadian rhythm and melatonin
- Neuroscience and Neural Engineering
- Connexins and lens biology
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Glaucoma and retinal disorders
- Mitochondrial Function and Pathology
- Traumatic Brain Injury and Neurovascular Disturbances
- Physiological and biochemical adaptations
- Thermal Regulation in Medicine
- Insect Resistance and Genetics
- Beetle Biology and Toxicology Studies
- Zebrafish Biomedical Research Applications
- Single-cell and spatial transcriptomics
- Heat shock proteins research
- Burn Injury Management and Outcomes
- Adipose Tissue and Metabolism
- Turtle Biology and Conservation
- bioluminescence and chemiluminescence research
- Aquaculture disease management and microbiota
Oil Crops Research Institute
2024-2025
Chinese Academy of Fishery Sciences
2024-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Shanghai Ocean University
2025
National Eye Institute
2015-2024
National Institutes of Health
2014-2024
Sun Yat-sen University
2017-2024
Third Affiliated Hospital of Sun Yat-sen University
2024
He University
2020
University of Chinese Academy of Sciences
2020
The retinal degenerative diseases, which together constitute a leading cause of hereditary blindness worldwide, are largely untreatable. Development reliable methods to culture complex tissues from human pluripotent stem cells (hPSCs) could offer means study development, provide platform investigate the mechanisms degeneration and screen for neuroprotective compounds, basis cell-based therapeutic strategies. In this study, we describe an in vitro method by hPSCs can be differentiated into 3D...
Abstract The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs 1 . Retinal cell types may have evolved to accommodate these varied needs, but this has not been systematically studied. Here we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a bird, reptile, teleost fish lamprey. We found high molecular conservation...
Color, an important visual cue for survival, is encoded by comparing signals from photoreceptors with different spectral sensitivities. The mouse retina expresses a short wavelength-sensitive and middle/long opsin (S- M-opsin), forming opposing, overlapping gradients along the dorsal-ventral axis. Here, we analyzed distribution of all cone types across entire two commonly used strains. We found, unexpectedly, that ‘true S-cones’ (S-opsin only) are highly concentrated (up to 30% cones) in...
Mammalian photoreceptors aggregate numerous mitochondria, organelles chiefly for energy production, in the ellipsoid region immediately adjacent to light-sensitive outer segment support high metabolic demands of phototransduction. However, these complex, lipid-rich are also poised affect light passage into segment. Here, we show, via live imaging and simulations, that despite this risk scattering or absorption, tightly packed mitochondria “focus” entry mitochondrial remodeling affects such...
Introduction Aeromonas hydrophila , a bacterium widely distributed in the natural environment, causes multiple diseases various animals. Exploring mechanism of host defense against A. can help develop efficient strategies infection. Methods Herein, we investigated temporal influence on Chinese soft-shelled turtle, an economically important species, at biochemical, transcriptomic, and metabolomic levels. Plasma parameters were detected with test kits. Transcriptome metabolome respectively...
Replacement of dysfunctional or dying photoreceptors offers a promising approach for retinal neurodegenerative diseases, including age-related macular degeneration and retinitis pigmentosa. Several studies have demonstrated the integration differentiation developing rod when transplanted in wild-type degenerating retina; however, physiology function donor cells are not adequately defined. Here, we describe physiological properties that tagged with green fluorescent protein (GFP) driven by...
Strategies aimed at invoking synaptic plasticity have therapeutic potential for several neurological conditions. The human retinal disease X-linked retinoschisis (XLRS) is characterized by impaired visual signal transmission through the retina and progressive acuity loss, mice lacking retinoschisin (RS1) recapitulate disease. Here, we demonstrate that restoration of RS1 via retina-specific delivery adeno-associated virus type 8-RS1 (AAV8-RS1) vector rescues molecular pathology...
The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs (Baden et al., 2020). One might expect that retinal cell types evolved to accommodate these varied needs, but this has not been systematically studied. Here, we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a teleost fish, bird, reptile lamprey. Molecular...
Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to shortage of effective and appropriate endogenous references, studies molecular functional genes in , have been tremendously limited. In this study, ten internal reference ( ACT ARF1 AK EF1α GAPDH α-TUB RPL6 RPL13 RPS3 RPS18 ) were tested assessed under four selected treatments including adult ages, tissues, temperatures, sex by RT-qPCR based five methods (Ct value, geNorm, NormFinder, BestKeeper...
Rod signals traverse several synapses en route to cone bipolar cells. In one pathway, rods communicate directly with cones via gap junctions. a second flow rods-rod bipolars-AII amacrines-cone bipolars. The relative contribution of each pathway retinal function is not well understood. Here we have examined this question from the perspective AII amacrine. AIIs form bidirectional electrical on Consequently, as bipolars are activated by outer plexiform inputs, they too should contribute...
Significance MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression posttranscriptionally. The evolutionarily conserved miR-183/96/182 cluster, consisting of three related miRNAs, is highly expressed in maturing sensory receptor cells. However, its role the functional maturation receptors has not been adequately addressed due to lack appropriate vivo models. We show deletion mice leads severe deficits vision, hearing, balance, and smell. These arise from defects timing...
Abstract Cold-induced injuries severely limit opportunities and outcomes of hypothermic therapies organ preservation, calling for better understanding cold adaptation. Here, by surveying cold-altered chromatin accessibility integrated CUT&Tag/RNA-seq analyses in human stem cells, we reveal forkhead box O1 (FOXO1) as a key transcription factor autonomous Accordingly, find nonconventional, temperature-sensitive FOXO1 transport mechanism involving the nuclear pore complex protein RANBP2,...
The Chinese soft-shelled turtle (Pelodiscus sinensis), a type of warm-water reptile, is frequently chosen as the model animal to understand how organisms respond environmental stressors. However, responsive mechanism P. sinensis natural cold stress unclear, especially in terms metabolic pattern and molecular pathways. Herein, plasma biochemical, hepatic morphological, apoptotic, transcriptomic, metabolomic detection methods were performed investigate response acute stress. A consistent...
We propose a standardized naming system for vertebrate visual photoreceptors (i.e., rods and cones) that reflects our current understanding of their evolutionary history. Vertebrate have been studied well over century, but fixed nomenclature referring to orthologous cell types across diverse species has lacking. Instead, variably - often confusingly named according morphology, presence/absence ‘rhodopsin,’ spectral sensitivity, chromophore usage, and/or the gene family opsin(s) they express....
The Chinese soft-shelled turtle (Pelodiscus sinensis), as a type of warm-water reptile, could be induced to massive death by sharp temperature decline. Hence, the mechanism spleen tissue responding cold stress in P. sinensis was investigated. present results showed that superoxide dismutase (SOD) activity declined from 4 16 days post-cold-stress (dps), while catalase (CAT) and glutathione peroxidase (GSH-Px) activities increased, 8 dps 14 °C (T14) 7 (T7) groups. transcriptome T7 group...
We propose a standardized naming system for vertebrate visual photoreceptors (i.e., rods and cones) that reflects our current understanding of their evolutionary history. Vertebrate have been studied well over century, but fixed nomenclature referring to orthologous cell types across diverse species has lacking. Instead, variably - often confusingly named according morphology, presence/absence ‘rhodopsin,’ spectral sensitivity, chromophore usage, and/or the gene family opsin(s) they express....