A5014084205- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Beetle Biology and Toxicology Studies
- Molecular Biology Techniques and Applications
- Animal Behavior and Reproduction
- Insect and Pesticide Research
- RNA Research and Splicing
- Reproductive biology and impacts on aquatic species
- Insect Utilization and Effects
- Forest Insect Ecology and Management
- Bacteriophages and microbial interactions
- Prion Diseases and Protein Misfolding
- Silk-based biomaterials and applications
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Antimicrobial Peptides and Activities
- Environmental DNA in Biodiversity Studies
- Adenosine and Purinergic Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
China Pharmaceutical University
2025
Ministry of Agriculture and Rural Affairs
2024-2025
Oil Crops Research Institute
2024-2025
Nanjing Agricultural University
2020-2023
Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to shortage of effective and appropriate endogenous references, studies molecular functional genes in , have been tremendously limited. In this study, ten internal reference ( ACT ARF1 AK EF1α GAPDH α-TUB RPL6 RPL13 RPS3 RPS18 ) were tested assessed under four selected treatments including adult ages, tissues, temperatures, sex by RT-qPCR based five methods (Ct value, geNorm, NormFinder, BestKeeper...
The P2X receptor family comprises ATP-gated non-selective cation channels central to physiological processes across the nervous, immune, cardiovascular, respiratory, and reproductive systems. While P2X1, P2X2, P2X3, P2X4, P2X7 are well-established as ATP-sensitive, P2X5 isoforms transcript variants (TVs) have traditionally been considered ATP-insensitive, limiting their functional exploration. This study identifies previously overlooked ATP-sensitive diverse species. Through gene profiling...
Abstract Henosepilachna vigintioctopunctata is a serious insect pest which attacks large number of nightshades and cucurbits in Asian countries, Brazil Australia. Prolonged application traditional pesticides has caused environmental pollution exerted deleterious effects on human health. Finding new approaches with high target specificity low contamination become an urgent task. RNA interference (RNAi) induced by double‐stranded (dsRNA) expected to be applicable managing this pest. Here we...
Due to a lack of effective internal references, studies on functional genes in Phthorimaea operculella, serious Lepidopteran pest attacking potatoes worldwide, have been greatly limited. To select suitable endogenous controls, ten housekeeping actin (ACT), α-tubulin (α-TUB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1α (EF1α), 18S and 28S ribosomal RNA (18S, 28S), protein RPL4, RPL13 RPL27 superoxide dismutase (SOD) were tested. Their expression levels determined...
Fushi Tarazu Factor 1 (FTZ-F1), a member of the nuclear receptor superfamily, is downstream factor 20-hydroxyecdysone signaling. In Drosophila melanogaster, alternative transcription start and splicing in FTZ-F1 gene generate αFTZ-F1 βFTZ-F1 isoforms, which are vital for pair-rule segmentation early embryogenesis post-embryonic development, respectively. However, whether same mRNA isoforms present exert conservative roles remains to be clarified other insects. paper, we first mined genomic...
Abstract In Drosophila melanogaster , ecdysis triggering hormone (ETH) is the key factor behaviour and promoting trachea clearance. However, whether ETH plays dual roles in non‐dipteran insects unknown. this survey, we found that Ldeth mRNA levels were positively correlated with circulating 20‐hydroxyecdysone (20E) titers Leptinotarsa decemlineata . Ingestion of an ecdysteroid agonist halofenozide or 20E stimulated transcription whereas RNA interference (RNAi) ecdysteroidogenesis ( LdPTTH...
Epicauta gorhami is a hypermetamorphic insect that mainly forage soybeans during the adult stage. However, lack of appropriate references hinders our studying gene function in E. gorhami. In this study, refer to five computational tools (Ct value, geNorm, NormFinder, BestKeeper and RefFinder), stability 10 housekeeping genes (GAPDH, ACT, RPL4, RPL27, α-TUB, RPS18, EF1α, RPS28, RPL13 SOD) was assessed by RT-qPCR under three different conditions (adult ages, tissues/organs...
Ecdysone-induced protein 93F (E93) plays triple roles during post-embryonic development in insects whose juvenile instars are more than four. However, it only acts as a specifier of adult structures Drosophila flies larval fixed at three. In this study, we determined the functions E93 eggplant lady beetle (Henosepilachna vigintioctopunctata), which has four instars. We uncovered that was abundantly expressed prepupal and pupal stages. A precocious inhibition hormone signal by RNA...
Insect ecdysis triggering hormone (ETH) receptors (ETHRs) are rhodopsin-like G protein-coupled receptors. Upon binding its ligand ETH, ETHR initiates a precisely programed behavior series and physiological events. In Drosophila melanogaster , the ethr gene produces two functionally distinct splicing isoforms, ethra ethrb . ETH/ETHRA activates eclosion (EH), kinin, crustacean cardioactive peptide (CCAP), bursicon (burs pburs) neurons, among others, in rigid order, to elicit behavioral...
RNA interference (RNAi) is a breakthrough technology in pest control. It highly efficient to Coleopteran pests such as the Colorado potato beetle Leptinotarsa decemlineata, serious defoliator mainly attacking potatoes worldwide. The first step for effective control by RNAi development of and reliable target genes.Our results revealed that continuous ingestion dsLdRan 3 days successfully silenced gene, inhibited larval growth killed 100% L. decemlineata larvae. When bioassay began at second-,...
Epicauta gorhami is a hypermetamorphic insect that mainly forage soybeans during the adult stage. However, lack of appropriate references hinders our studying gene function in E. gorhami. In this study, referring to five computational tools (Ct value, geNorm, NormFinder, BestKeeper and RefFinder), stability 10 housekeeping genes (GAPDH, ACT, RPL4, RPL27, α-TUB, RPS18, EF1α, RPS28, RPL13 SOD) was assessed by qRT-PCR under three different conditions (adult ages, tissues/organs temperatures)....
Dusky-like (Dyl) is a transmembrane protein containing zona pellucida domain. Its physiological roles during metamorphosis have been well explored in Drosophila melanogaster and also documented Tribolium castaneum. However, Dyl has undergone functional shift between Diptera Coleoptera insects. Further investigation of other insects will be helpful to further clarify its function insect growth development. Henosepilachna vigintioctopunctata an important that causes enormous economic losses...