A5080379725- Microbial Inactivation Methods
- Freezing and Crystallization Processes
- Heat Transfer and Optimization
- Heat Transfer and Boiling Studies
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Magnetic and Electromagnetic Effects
- Lipid Membrane Structure and Behavior
- Spectroscopy Techniques in Biomedical and Chemical Research
- Microencapsulation and Drying Processes
- Food Industry and Aquatic Biology
- Reproductive Biology and Fertility
- Electrospun Nanofibers in Biomedical Applications
- Nanopore and Nanochannel Transport Studies
- Spectroscopy and Quantum Chemical Studies
- Pancreatic function and diabetes
- Methane Hydrates and Related Phenomena
- Refrigeration and Air Conditioning Technologies
- Tissue Engineering and Regenerative Medicine
- Electrohydrodynamics and Fluid Dynamics
- thermodynamics and calorimetric analyses
- Advanced Thermodynamic Systems and Engines
- Hemoglobin structure and function
- Biopolymer Synthesis and Applications
- Food Drying and Modeling
The University of Tokyo
2015-2025
Advanced Institute of Industrial Technology
2005-2018
Tokyo University of Science
1998-2013
University of Würzburg
2002-2005
The COVID-19 pandemic, spanning from early 2020 to late 2022, posed unprecedented challenges for global public health. However, it also spurred innovative approaches pandemic management, notably the development of pathogen detection in wastewater. It was successfully demonstrated that wastewater analysis can not only reflect ongoing infections but serve as an indicator disease prevalence within communities. Recognizing value longitudinal analyses various pathogens, we identified need...
This article provides an update on the current state of decarbonization efforts in biobanking, reflecting evolving discussions at annual meetings International Society for Biological and Environmental Repositories (ISBER). Following roundtable 2021 2023 meetings, 2024 ISBER meeting, which was held Melbourne, Australia, featured a symposium, workshop, posters dedicated to decarbonization, highlighting its growing significance global scale. The introduction term "green biobanking" this meeting...
Swelling-activated pathways for myo-inositol, one of the most abundant organic osmolytes in mammalian cells, have not yet been identified. The present study explores SLC5A3 protein as a possible transporter myo-inositol hyponically swollen HEK293 cells. To address this issue, we examined relationship between hypotonicity-induced changes plasma membrane permeability to Pino [m/s] and expression/localization SLC5A3. values were determined by cell volumetry over wide tonicity range (100–275...
Abstract In the dielectric spectroscopy of aqueous systems containing ions, loss spectrum is affected by contribution direct current (DC) conductivity σ , which sometimes significantly overlaps relaxations related to slow dynamics water molecules distributing in sub-GHz frequency range. We have demonstrated a practical procedure based on Kramers–Kronig (KK) relations exclude DC from experimentally obtained spectrum. Examples KK transform for ultrapure and solution protein (lysozyme) showed...
In the present paper, heat transport rates and fluid flow patterns of meandering closed-loop heat-transport device (MCL-HTD) were experimentally analytically investigated by using water as an operating liquid. MCL-HTD consists a closed-looped-tube between heating cooling parts with liquid volume fraction in it. First, effective thermal conductivity was measured, which showed much higher value than that copper. Next, observation conducted, simple periodic pattern appeared at high This...
In this study, the relationship between deterioration of enzymatic activity protein in a preservative solution and molecular mobility was experimentally investigated by dielectric spectroscopy test. Dielectric used to observe mobilities solutions including various ratios two protective agents, trehalose ε-poly-l-lysine, at 40 °C. We also examined l-lactate dehydrogenase (LDH) °C for days obtain rate LDH same solutions. Our experimental results reveal that (1) three relaxation times water...
Water dynamics is essential to biochemical processes by mediating all such reactions, including biomolecular degeneration in solutions. To disentangle the molecular-scale distribution of water around a solute biomolecule, we investigated here rotational lysozyme combining molecular (MD) simulations and broadband dielectric spectroscopy (BDS). A statistical analysis using relaxation times trajectories every single molecule was proposed, two-dimensional probability at distance from surface...
Here we describe a new method to deliver membrane impermeable cryo-/lyo-protective agents (CPAs) into the cytosol of living cells via their electrofusion with Giant Unilamellar Vesicles (GUVs) containing large amounts CPAs. Membrane is commonly believed be triggered by irreversible electrical breakdown at contact region induced DC field pulse. Therefore, analysis temporal changes potential distribution in cell-GUV pair necessary achieve optimum conditions respect pulse strength and duration....
By near-infrared spectroscopy, we classified water molecules in hydrated gelatin membranes a drying process. Absorbance spectra the frequency range of 4500–5500 cm−1 were resolved into three peaks, S0, S1, and S2, that correspond to with different hydrogen bond states. From areas absorbance peaks as function content gelatin, together information on freezing properties measured by differential scanning calorimetry, found that, when is less than 20%, free disappears only weakly strongly bound...
Microinjection with ultra-fine glass capillaries is widely used to introduce cryoprotective agents and other foreign molecules into animal cells, oocytes, embryos. The fragility of makes difficult the microinjection fish eggs embryos, which are usually protected by a hard outer shell, called chorion. In this study, we new electromechanical approach, based on electropiercing stationary needle electrode. setup consists two asymmetric electrodes, including μm-scaled nickel placed opposite...
Abstract Deterioration of biomaterial is subject to the interaction between biomolecules and water, which often special interest realization long‐term preservation. In previous study, we reported relationship dielectric rotational relaxation time ν2 + ν3 NIR combination band water as well theory behind it using saccharide solutions examples proposed a method accordingly takes advantages infrared microscope measure in biomaterials. this work, examined protein, polypeptide, sugar alcohol some...
We here focus on whether a model peptide for Group 3 LEA (G3LEA) proteins can be used the dry preservation of vesicles whose size and phospholipid compositions resemble those living cells. For this purpose, we prepared called PvLEA-22, which consists two tandem repeats 11-mer motif characteristic G3LEA from an African sleeping chironomid, giant (diameter 6–9 µm) with egg phosphatidylcholine. examined particle distribution before after drying rehydration in presence peptide. The suppressed...
Water rotational dynamics in biomolecular solution is crucial to evaluating and controlling biomolecule stability. In this molecular simulation (MD) study on lysozyme solutions, we present how the exerted internal electric field determines water dynamics. We find that relaxation time of rotation equivalent reorientation overall for every single molecule, regardless its translation mode. Namely, synchronizes with reorientation. also map process at fixed points relative protein solution, which...