A5055860157- Innovative Microfluidic and Catalytic Techniques Innovation
- Enzyme Catalysis and Immobilization
- Microfluidic and Capillary Electrophoresis Applications
- Steroid Chemistry and Biochemistry
- Viral Infectious Diseases and Gene Expression in Insects
- 3D Printing in Biomedical Research
- Microbial Metabolic Engineering and Bioproduction
- Pharmacogenetics and Drug Metabolism
- Microfluidic and Bio-sensing Technologies
- Crystallization and Solubility Studies
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Additive Manufacturing and 3D Printing Technologies
- RNA and protein synthesis mechanisms
- Matrix Theory and Algorithms
- Parallel Computing and Optimization Techniques
- Analytical Chemistry and Sensors
- Protein purification and stability
- COVID-19 Clinical Research Studies
- Electrochemical sensors and biosensors
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Pluripotent Stem Cells Research
- thermodynamics and calorimetric analyses
- Complement system in diseases
University College London
2014-2024
Arbed (Luxembourg)
2023
Biochemical Society
2023
Transnational Press London
2017
University of Lisbon
2007-2014
Instituto Superior Técnico
2007-2012
Institute for Biotechnology and Bioengineering
2007-2011
Instituto Politécnico de Lisboa
2011
Universitat Jaume I
2002-2011
Universidade Nova de Lisboa
2006
Messenger RNA (mRNA) vaccines are a new alternative to conventional with prominent role in infectious disease control. These produced vitro transcription (IVT) reactions, catalyzed by polymerase cascade reactions. To ensure an efficient and cost-effective manufacturing process, essential for large-scale production effective vaccine supply chain, the IVT reaction needs be optimized. is complex that contains large number of variables can affect its outcome. Traditional optimization methods...
Microreactors have been successfully applied to execute a broad range of biotransformations in flow. However, microreactors typically designed with specific biotransformation or biocatalyst immobilization method mind, constraining their wider applicability. Furthermore, design is either applicable for whole-cell enzyme biocatalysis, but not both. We present novel microreactor which offers cartridge-like insertion both immobilised enzymes and cells. A T-shaped lid opens closes the reaction...
Abstract Advances in high‐throughput process development and optimization involve the rational use of miniaturized stirred bioreactors, instrumented shaken flasks microtiter plates. As expected, each one provides different levels control monitoring, requiring a compromise between data quantity quality. Despite recent advances, traditional with nominal volumes below 250 mL plates are still widely used to assemble wide arrays biotransformation/bioconversion data, because their simplicity low...
The manufacturing of mRNA vaccines relies on cell-free based systems that are easily scalable and flexible compared with the traditional vaccine processes. Typically, standard processes yield 2 to 5 g L
Abstract Rapid biocatalytic process development and intensification continues to be challenging with currently available methods. Chiral amino‐alcohols are of particular interest as they represent key industrial synthons for the production complex molecules optically pure pharmaceuticals. (2 S ,3 R )‐2‐amino‐1,3,4‐butanetriol (ABT), a building block synthesis protease inhibitors detoxifying agents, can synthesized from simple, non‐chiral starting materials, by coupling transketolase‐...
Abstract Oxygen plays a key role in stem cell biology as signaling molecule and an indicator of energy metabolism. Quantification cellular oxygen kinetics, i.e. the determination specific uptake rates (sOURs), is routinely used to understand metabolic shifts. However current methods determine sOUR adherent cultures rely on sampling, which impacts phenotype. We present real‐time monitoring growth from phase contrast microscopy images, respiration using optical sensors for dissolved oxygen....
Monitoring and control of pH is essential for the reaction conditions progress any biocatalytic or biotechnological process. Microfluidic enzymatic reactors are increasingly proposed process development, however typically lack instrumentation, such as monitoring. We present a microfluidic side-entry reactor (μSER) demonstrate first time real-time monitoring progression an in step towards achieving control. Two different types optical sensors were integrated at several positions channel which...
Poly(methyl methacrylate) (PMMA) microfluidic devices have become promising platforms for a wide range of applications. Here we report simple method immobilising histidine-tagged enzymes suitable PMMA devices. The 1-step-immobilisation described is based on the affinity His-tag/Ni-NTA interaction and does not require prior amination surface, unlike many existing protocols. We compared it with 3-step immobilisation protocol involving linking NTA via glutaraldehyde cross-linker. These methods...
Abstract BACKGROUND: The aim of this study was to evaluate the feasibility enzyme immobilization in PVA particles through extrusion LentiKat ® Liquid polyethylene glycol. Inulinase, with invertase activity for sucrose hydrolysis, used as model system. RESULTS: Inulinase effectively immobilized particles. pH optimum broadened lower values. Mechanical instability under prolonged incubation above 55 °C observed. A 1.8‐fold increase apparent K M (Michaelis constant) suggests diffusion...
Microscale technology has been increasingly used in chemical synthesis up to production scale, but biocatalysis the implementation proceeding at a slower pace. In this work, design of low cost and versatile continuous flow enzyme microreactor is described that illustrates potential microfluidic reactors for both development characterization biocatalytic processes. The core structure developed reactor consists an array capillaries with 450 μm inner diameter their surface functionalized...