A large-area high-density nanoscale Lycurgus cup array is created with 100 times better sensitivity than any other reported nanoplasmonic device. With this device, spectroscopy sensing, for the first time, becomes colorimetric sensing requiring only naked eye or ordinary visible color photography.

Photodynamic and photothermal therapies are promising treatments for cancer, dermatological, ophthalmological conditions. However, photodynamic therapy (PDT) is less effective in oxygen-deficient tumor environments. Combining PDT with (PTT) can enhance oxygen supply treatment efficacy. Inorganic PTT agents pose toxicity risks, limiting their clinical use despite high performance. In this study, we developed a novel nanomedicine integrating an all-organic agent organic photosensitizer,...

Lab-on-chip medical diagnostics in a global health setting would greatly benefit from highly portable, cost effective and readily available devices. Digital compact disc (CD) the corresponding detection device—CD drives—for personal computers are extremely affordable distributable worldwide, therefore they can be immediately used applications if empowered with molecular cellular biosensing functions. Here we present novel digital microfluidic CD device derived conventional music or data...

Organ-on-a-chip (OoC) technology has experienced exponential growth driven by the need for a better understanding of in-organ processes and development novel approaches. This paper investigates compares flow behavior filling characteristics two microfluidic liver-on-a-chip devices using Computational Fluid Dynamics (CFD) analysis experimental cell culture based on Huh7 line. The conducted computational analyses chips showed that elliptical chamber chip proposed herein offers improved in...

Poly(methyl methacrylate) (PMMA) microfluidic devices have become promising platforms for a wide range of applications. Here we report simple method immobilising histidine-tagged enzymes suitable PMMA devices. The 1-step-immobilisation described is based on the affinity His-tag/Ni-NTA interaction and does not require prior amination surface, unlike many existing protocols. We compared it with 3-step immobilisation protocol involving linking NTA via glutaraldehyde cross-linker. These methods...

The drug development process can greatly benefit from liver-on-a-chip platforms aiming to recapitulate the physiology, mechanisms, and functionalities of liver cells in an vitro environment. is most important organ metabolism investigation. Here, we report a hybrid cyclic olefin copolymer (COC) polydimethylsiloxane (PDMS) microfluidic (HCP) platform culture Huh7 hepatoma cell line dynamic conditions towards system. comprised COC bottom layer with microchannel PDMS-based flat top sandwiched...

Gelatin nanoparticles coated with Cathepsin D-specific peptides were developed as a vehicle for the targeted delivery of cancer drug doxorubicin (DOX) to treat breast malignancy. D, cell secretion enzyme, triggered release DOX by digesting protective peptide-coating layer nanoparticles. Fabricated successfully detected ultrasound imaging in both vitro conditions and vivo mouse models. Cell viability experiments conducted determine efficacy biomarker activation specific lines. These...

The correlation between shape and concentration of silver nanoparticles (AgNPs), their cytotoxicity formation reactive oxygen species (ROS) in the presence electromagnetic fields (EMFs) has been investigated. In addition, bio-effects caused by combination EMFs graphene (GrNPs) have also assessed. AgNPs three shapes (triangular, spherical colloidal) GrNPs were added high concentrations to culture human fibroblasts exposed EMF different frequencies: 900, 2400 7500 MHz. results demonstrated...

Here, we report a fluorescent electrospun nanofiber membrane for integration into microfluidic devices towards lung-on-a-chip applications complemented with the results of computational fluid dynamics modelling. A proposed hybrid poly(ε-caprolactone) (PCL)-collagen was developed, characterized, tested, and integrated prototype chip biocompatibility studies. The resulting has thickness approximately 10 μm, can be adjusted appropriate porosity, offers excellent mimicry basement to used in...

A nanocup structure that mimics the Lycurgus cup effect enables nanoplasmonic spectroscopy to be used for colorimetric sensing, requiring only naked eye or ordinary visible color photography. Enhanced optical transmission from subwavelength structures couples with localized surface plasmon metal nanoparticles on side walls of cup, creating extraordinary sensitivity and changes. Utility sensor chemical imaging, biomolecular integration portable microfluidics devices lab-on chip-applications...

Here, we present the development of first prototype a microfluidic chip with two compartments and nanofiber membrane for mimicking air-blood barrier toward integration into device use in lung-on-a-chip applications. A microchannels air cell culture media was developed. Thin film membranes have been developed fabricated comparison standard material reported devices. In this paper, preliminary results on design chip, thin-film membranes, electrospun membranes. Nanofiber made polycaprolactone...

Background — The presence of chronic hard-to-heal wounds significantly affects patients’ quality life causing pain, discomfort, decrease mobility and consequently leads to social isolation depression. This type wound is more prevalent among older population. It has been estimated that up 1-2% the world population have this condition. treatment expensive long-term process, and, stresses healthcare systems every country. Unfortunately, limited information available about situation with in...

In this paper, we report a microfluidic polymer organ-on-a-chip device integrated with fiber optic sensor towards online monitoring of cell viability on-chip. As preliminary model applications, absorbance-based measurements various concentrations sucrose solution and AlluraRed dye in the chip were carried out. Absorbance peaks correlated to solutions analyzed. Accurate sensing analytes continuous flow through was achieved. Fiber can be used as label-free assay for other applications based on...

Microfluidic organs-on-chips or microphysiological systems (MPS) are promising tools that can potentially replace animal testing in drug development. MPS platforms with microchannels seeded certain organ cells used to emulate vivo environments laboratory conditions. Among them, lung called lung-on-chip devices evaluate the influence of toxic particles, gases, and chemicals on tissue vitro. Lung-on-chip allow mimicry healthy conditions a wide range dis-eases (asthma, cancer, autoimmune,...

Introduction. Nanoparticles made of noble metals, such as gold and silver, have a great potential to be effectively employed for wound management. The nano-size particles provides an opportunity enlarge the contacting area, which results in more effective anti-bacterial action faster repair. It must noted that shape nanoparticles might play crucial role manifestation their anti-microbial properties. modern state technology allows fabrication with desired physical In order provide efficacy...

In this paper, we propose a new biocompatible PCL-collagen nanofiber membrane that could be integrated into microfluidic lung-on-a-chip device prototype. Membrane structure and properties were investigated using Scanning Electron Microscope (SEM), Fourier Transform Infrared (FTIR) spectroscopy, Mechanical tests, Contact angle measurements. Biocompatibility cytotoxicity of the on A549 adenocarcinoma human alveolar basal epithelial cell line Presto Blue Live/Dead assay. Additionally, sample...