В. А. Шепелев
A5060525262- Radio Astronomy Observations and Technology
- Astrophysics and Cosmic Phenomena
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Astronomical Observations and Instrumentation
- Galaxies: Formation, Evolution, Phenomena
- Geophysics and Gravity Measurements
- Relativity and Gravitational Theory
- Genomics and Chromatin Dynamics
- Superconducting and THz Device Technology
- RNA and protein synthesis mechanisms
- Electrical and Electromagnetic Research
- Radio Wave Propagation Studies
- Cosmology and Gravitation Theories
- Advanced Control and Stabilization in Aerospace Systems
- Genomic variations and chromosomal abnormalities
- Solar and Space Plasma Dynamics
- Gyrotron and Vacuum Electronics Research
- Advanced Research in Science and Engineering
- RNA modifications and cancer
- RNA Research and Splicing
- Superconducting Materials and Applications
- Antenna Design and Optimization
- Primate Behavior and Ecology
- Space exploration and regulation
Institute of Molecular Genetics
2007-2025
Russian Academy of Sciences
1996-2024
Vavilov Institute of General Genetics
2015-2022
National Academy of Sciences of Ukraine
2001-2021
Institute of Radio Astronomy
2002-2021
Irkutsk State Medical University
2021
Kurchatov Institute
2015-2019
Institute of Cytology and Genetics
2015
Bioinformatics Institute
2005-2007
University of Toledo Medical Center
2006
Existing human genome assemblies have almost entirely excluded repetitive sequences within and near centromeres, limiting our understanding of their organization, evolution, functions, which include facilitating proper chromosome segregation. Now, a complete, telomere-to-telomere assembly (T2T-CHM13) has enabled us to comprehensively characterize pericentromeric centromeric repeats, constitute 6.2% the (189.9 megabases). Detailed maps these regions revealed multimegabase structural...
Abstract Human centromeres have been traditionally very difficult to sequence and assemble owing their repetitive nature large size 1 . As a result, patterns of human centromeric variation models for evolution function remain incomplete, despite being among the most rapidly mutating regions 2,3 Here, using long-read sequencing, we completely sequenced assembled all from second genome compared it finished reference 4,5 We find that two sets show at least 4.1-fold increase in single-nucleotide...
Abstract Apes possess two sex chromosomes—the male-specific Y chromosome and the X chromosome, which is present in both males females. The crucial for male reproduction, with deletions being linked to infertility 1 . vital reproduction cognition 2 Variation mating patterns brain function among apes suggests corresponding differences their chromosomes. However, owing repetitive nature incomplete reference assemblies, ape chromosomes have been challenging study. Here, using methodology...
This paper describes digital radio astronomical receivers used for decameter and meter wavelength observations. Since 1998, performing on-the-fly dynamic spectrum calculations or waveform data recording without loss have been at the UTR-2 telescope, URAN VLBI system, GURT new generation telescope. Here, we detail these developed operation in strong interference environment that prevails range. Data collected with allowed us to discover numerous objects phenomena low frequencies, a summary of...
The human Y chromosome has been notoriously difficult to sequence and assemble because of its complex repeat structure including long palindromes, tandem repeats, segmental duplications 1–3 . As a result, more than half the is missing from GRCh38 reference it remains last be finished 4, 5 Here, Telomere-to-Telomere (T2T) consortium presents complete 62,460,029 base pair HG002 genome (T2T-Y) that corrects multiple errors in GRCh38-Y adds over 30 million pairs reference, revealing ampliconic...
Alpha satellite domains that currently function as centromeres of human chromosomes are flanked by layers older alpha satellite, thought to contain dead primate progenitors, which lost their and the ability homogenize repeats, upon appearance a new centromere. Using cladistic analysis monomers, we elucidated complete layer patterns on 8, 17, X related them each other satellites. We show discrete chronologically ordered partially symmetrical around an active centromere succession is shared in...
We completely sequenced and assembled all centromeres from a second human genome used two reference sets to benchmark genetic, epigenetic, evolutionary variation within diversity panel of humans apes. find that centromere single-nucleotide can increase by up 4.1-fold relative other genomic regions, with the caveat 45.8% centromeric sequence, on average, cannot be reliably aligned current methods due emergence new α-satellite higher-order repeat (HOR) structures threefold differences in...
ABSTRACT The crab-eating macaques ( Macaca fascicularis ) and rhesus M. mulatta are widely studied nonhuman primates in biomedical evolutionary research. Despite their significance, the current understanding of complex genomic structure differences between species requires substantial improvement. Here, we present a complete genome assembly macaque 20 haplotype-resolved assemblies to investigate regions major species. Segmental duplication is ∼42% lower, while centromeres ∼3.7 times longer...
Centromeric alpha satellite (AS) is composed of highly identical higher-order DNA repetitive sequences, which make the standard assembly process impossible. Because this AS repeats were severely underrepresented in previous versions human genome showing large centromeric gaps. The latest hg38 (GCA_000001405.15) employed a novel method approximate representation these sequences using reference models to fill Therefore, lot more assembled became available for genomic analysis. We used PERCON...
Abstract Apes possess two sex chromosomes—the male-specific Y and the X shared by males females. The chromosome is crucial for male reproduction, with deletions linked to infertility. carries genes vital reproduction cognition. Variation in mating patterns brain function among great apes suggests corresponding differences their structure evolution. However, due highly repetitive nature incomplete reference assemblies, ape chromosomes have been challenging study. Here, using state-of-the-art...
In the latest hg38 human genome assembly, centromeric gaps has been filled in by alpha satellite (AS) reference models (RMs) which are statistical representations of homogeneous higher-order repeat (HOR) arrays that make up bulk regions. We analyzed these to compose an atlas AS HORs where each monomer a HOR was represented number its polymorphic sequence variants. combined data and HMMER analysis platform annotate assembly. This led discovery new type low copy highly divergent were not RMs....
Abstract Motivation: Using bioinformatic approaches we aimed to characterize poorly understood abnormalities in splicing known as exon scrambling, repetition and trans-splicing. Results: We developed a software package that allows large-scale comparison of all human expressed sequence tags (EST) sequences the entire set gene sequences. Among 5 992 495 EST sequences, 401 cases 416 scrambling were found. The vast majority identified ESTs contain fragments rather than full-length repeated or...
Abstract Existing human genome assemblies have almost entirely excluded highly repetitive sequences within and near centromeres, limiting our understanding of their sequence, evolution, essential role in chromosome segregation. Here, we present an extensive study newly assembled peri/centromeric representing 6.2% (189.9 Mb) the first complete, telomere-to-telomere assembly (T2T-CHM13). We discovered novel patterns repeat organization, variation, evolution at both large small length scales....
Background: The skills required for neurosurgical operations using microsurgical techniques in a deep operating field are difficult to master the room without risk patients. Although there many training models, most do not use skull model simulate field. To solve this problem, 3D models were created provide increased laboratory before room, improving patient safety. Methods: A patient’s head was scanned computed tomography. data reconstructed and converted into standard printing file....
The paper presents detailed definitions, anatomical features and analytical data on the epidemiology etiopathogenesis of isolated lumbar cervical spinal stenoses their combinations (tandem stenoses). Most widely used classifications stenosing processes spine, modern X-ray imaging methods for diagnosis stenosis approaches to surgical treatment are described.
УДК 520.274; 523.9 Предложен комплекс аппаратуры для определения угловых характеристик источников, связанных с солнечным радиоизлучением на декаметровых волнах. Метод измерений основан интерферометрическом подходе использованием секционной структуры радиотелескопа УТР-2. Описана простая методика наблюдений и обработки данных, не требующая калибровок антенны. Проведены наблюдения спокойного Солнца всплесков его спорадической активности, позволившие определить как угловые размеры так их...