A5068471387- Wound Healing and Treatments
- Diabetic Foot Ulcer Assessment and Management
- Pressure Ulcer Prevention and Management
- Skin and Cellular Biology Research
- Mesenchymal stem cell research
- Diagnosis and Treatment of Venous Diseases
- Dermatology and Skin Diseases
- Skin Protection and Aging
- Dermatologic Treatments and Research
- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- Hair Growth and Disorders
- Surgical Sutures and Adhesives
- Tendon Structure and Treatment
- melanin and skin pigmentation
- Periodontal Regeneration and Treatments
- Silk-based biomaterials and applications
- Bacterial biofilms and quorum sensing
- Extracellular vesicles in disease
- Antimicrobial Peptides and Activities
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pluripotent Stem Cells Research
- Antimicrobial Resistance in Staphylococcus
- Bee Products Chemical Analysis
- Advancements in Transdermal Drug Delivery
University of Miami
2016-2025
Centro Tecnológico del Mar
2023
Dr. John T. Macdonald Foundation
2016-2022
Council of Science Editors
2017-2021
Cells Therapy (Poland)
2021
University of Miami Hospital
2017
University of Miami Health System
2016-2017
Advanced Dermatology
2012-2017
NYU Langone Health
1996-2017
In-Q-Tel
2014-2016
Understanding the pathology resulting from Staphylococcus aureus and Pseudomonas aeruginosa polymicrobial wound infections is of great importance due to their ubiquitous nature, increasing prevalence, growing resistance antimicrobial agents, ability delay healing. Methicillin-resistant S. USA300 leading cause community-associated bacterial in increased morbidity mortality. We utilized a well-established porcine partial thickness healing model study synergistic effects P. on Wound...
Chronic wounds, such as venous ulcers, are characterized by physiological impairments manifested delays in healing, resulting severe morbidity. Surgical debridement is routinely performed on chronic wounds because it stimulates healing. However, procedures repeated many times the same patient because, contrast to tumor excision, there no objective biological/molecular markers guide extent of debridement. To develop bioassays that can potentially surgical debridement, we assessed pathogenesis...
Older adults are more likely to have chronic wounds than younger people, and the effect of on quality life is particularly profound in this population. Wound healing slows with age, but basic biology underlying influence age‐associated changes wound poorly understood. Most studies used vitro approaches various animal models, observed translate human conditions. The age accompanying multimorbidity effectiveness existing emerging treatment for also unknown, older tend be excluded from...
Abstract Diabetic foot ulcers (DFUs) are a life-threatening disease that often result in lower limb amputations and shortened lifespan. However, molecular mechanisms contributing to the pathogenesis of DFUs remain poorly understood. We use next-generation sequencing generate human dataset pathogenic compare transcriptional profiles skin oral acute wounds, as model “ideal” adult tissue repair due accelerated closure without scarring. Here we identify major networks deregulated decreased...
The incidence of chronic wounds is increased among older adults, and the impact on quality life particularly profound in this population. It well established that wound healing slows with age. However, basic biology underlying influence age-associated changes are poorly understood. Most studies have used vitro approaches various animal models, but observed translate to human conditions. age accompanying multi-morbidity effectiveness existing emerging treatment for also unknown, adults tend...
Glucocorticoids (GCs) are known inhibitors of wound healing. In this study we report the novel finding that both keratinocytes in vitro and epidermis vivo synthesize cortisol how synthesis regulates We show expresses enzymes essential for synthesis, including steroid 11 β-hydroxylase (CYP11B1), an enzyme controls negative feedback mechanism, 11β-hydroxysteroid dehydrogenase 2 (11βHSD2). also found skin can be stimulated by ACTH inhibited metyrapone (CYP11B1 inhibitor). Interestingly, IL-1β,...
Glucocorticoids (GCs) have a long history of use as therapeutic agents for numerous skin diseases. Surprisingly, their specific molecular effects are largely unknown. To characterize GC action in epidermis, we compared the transcriptional profiles primary human keratinocytes untreated and treated with dexamethasone (DEX) 1, 4, 24, 48, 72 h using large scale microarray analyses. The majority genes were found to be regulated only after 24 remained throughout treatment. In addition regulation...
Transforming growth factor β (TGFβ) is important in inflammation, angiogenesis, reepithelialization and connective tissue regeneration during wound healing. We analyzed components of TGFβ signaling pathway biopsies from 10 patients with nonhealing venous ulcers (VUs). Using comparative genomics transcriptional profiles VUs TGFβ-treated keratinocytes, we found deregulation target genes VUs. quantitative polymerase chain reaction (qPCR) immunohistochemical analysis, suppression TGFβRI, TGFβRII...
Abstract Epidermal morphology of chronic wounds differs from that normal epidermis. Biopsies non‐healing edges obtained patients with venous ulcers show thick and hyperproliferative epidermis mitosis present in suprabasal layers. This is also hyper‐keratotic parakeratotic. suggests incomplete activation differentiation keratinocytes. To identify molecular changes lead to pathogenic alterations keratinocyte pathways we isolated mRNA deriving determined transcriptional profiles using...