A5018317562- Spine and Intervertebral Disc Pathology
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Wound Healing and Treatments
- Musculoskeletal pain and rehabilitation
- T-cell and B-cell Immunology
- Anesthesia and Pain Management
- Dermatologic Treatments and Research
- Vitamin C and Antioxidants Research
- Periodontal Regeneration and Treatments
- Mast cells and histamine
- Tendon Structure and Treatment
- Hair Growth and Disorders
- Spondyloarthritis Studies and Treatments
- Bee Products Chemical Analysis
- Bone Metabolism and Diseases
- Cervical and Thoracic Myelopathy
- Dermatology and Skin Diseases
- Skin Protection and Aging
- Natural Antidiabetic Agents Studies
- Orthopedic Infections and Treatments
- Hormonal Regulation and Hypertension
- Retinoids in leukemia and cellular processes
- TGF-β signaling in diseases
- Medicinal Plants and Bioactive Compounds
Hospital for Special Surgery
2008-2023
Rutgers, The State University of New Jersey
2019-2022
Johnson University
2022
Cornell University
2009
Glucocorticoids (GCs) are known inhibitors of wound healing. In this study we report the novel finding that both keratinocytes in vitro and epidermis vivo synthesize cortisol how synthesis regulates We show expresses enzymes essential for synthesis, including steroid 11 β-hydroxylase (CYP11B1), an enzyme controls negative feedback mechanism, 11β-hydroxysteroid dehydrogenase 2 (11βHSD2). also found skin can be stimulated by ACTH inhibited metyrapone (CYP11B1 inhibitor). Interestingly, IL-1β,...
Transforming growth factor β (TGFβ) is important in inflammation, angiogenesis, reepithelialization and connective tissue regeneration during wound healing. We analyzed components of TGFβ signaling pathway biopsies from 10 patients with nonhealing venous ulcers (VUs). Using comparative genomics transcriptional profiles VUs TGFβ-treated keratinocytes, we found deregulation target genes VUs. quantitative polymerase chain reaction (qPCR) immunohistochemical analysis, suppression TGFβRI, TGFβRII...
Abstract Epidermal morphology of chronic wounds differs from that normal epidermis. Biopsies non‐healing edges obtained patients with venous ulcers show thick and hyperproliferative epidermis mitosis present in suprabasal layers. This is also hyper‐keratotic parakeratotic. suggests incomplete activation differentiation keratinocytes. To identify molecular changes lead to pathogenic alterations keratinocyte pathways we isolated mRNA deriving determined transcriptional profiles using...
Abstract Retinoids (RA) have been used as therapeutic agents for numerous skin diseases, from psoriasis to acne and wrinkles. While RA is known inhibit keratinocyte differentiation, the molecular effects of in epidermis not comprehensively defined. To identify transcriptional targets primary human epidermal keratinocytes, we compared profiles cells grown presence or absence all‐trans retinoic acid 1, 4, 24, 48, 72 h, using large DNA microarrays. As expected, suppresses protein markers...
Abstract The epidermis is maintained by epidermal stem cells ( ESC s) that reside in distinct niches and contribute to homeostasis wound closure. Keratinocytes at the nonhealing edges of venous ulcers VU are healing‐incompetent, hyperproliferative, nonmigratory, suggesting deregulation s. To date, genes which regulate have been studied mice only. Utilizing microarray analysis edges, we identified changes expression harboring regulation ESCs their fate. In a prospective clinical study 10 s,...
Farnesyl pyrophosphate (FPP), a key intermediate in the mevalonate pathway and protein farnesylation, can act as an agonist for several nuclear hormone receptors. Here we show novel mechanism by which FPP inhibits wound healing acting glucocorticoid receptor (GR). Elevation of endogenous squalene synthetase inhibitor zaragozic acid A (ZGA) or addition to cell culture medium results activation translocation GR, known inhibitor. We used functional studies evaluate effects on healing. Both ZGA...
The expression of BTB-ZF transcription factors such as ThPOK in CD4 + T cells, Bcl6 follicular helper and PLZF natural killer cells defines the fundamental nature characteristics these cells. Screening for lineage-defining genes led to discovery a subset that expressed Zbtb20. About half Zbtb20 FoxP3, factor regulatory (T regs ). were phenotypically genetically distinct from larger conventional reg population. constitutively mRNA interleukin-10 produced high levels cytokine upon primary...
Abstract Spontaneous mineralization of the nucleus pulposus (NP) has been observed in cases intervertebral disc degeneration (IDD). Inflammatory cytokines have implicated multiple tissues through their modulation expression factors that enable or inhibit mineralization, including TNAP, ANKH ENPP1. This study examines underlying leading to NP focusing on contribution inflammatory cytokine, TNF, this pathologic event. We show human and bovine primary cells express high levels ENPP1, low...
Abstract Basophils are innate immune cells associated with type 2 immunity, allergic reactions, and host defense against parasite infections. In this study, we show that the transcription factor PLZF, which is known for its essential role in function development of several lymphocyte subsets, also important myeloid-derived basophil lineage. PLZF-deficient mice had decreased numbers progenitors bone marrow mature basophils multiple peripheral tissues. Functionally, were less responsive to IgE...
Bone morphogenetic protein (BMP) gene delivery to Lewis rat lumbar intervertebral discs (IVDs) drives bone formation anterior and external the IVD, suggesting IVD is inhospitable osteogenesis. This study was designed determine if destruction with a proteoglycanase, and/or generating an blood supply by of angiogenic growth factor, could render permissive intra-discal BMP-driven osteogenesis fusion. Surgical naïve or gene-programmed cells (BMP2/BMP7 co-expressing VEGF165 expressing) +/-...
Abstract The BTB-ZF genes are a family of 49 transcription factors that control the lineage specification and development key effector functions different subsets lymphocytes. Using single-cell factor expression analysis, we identified unique subset T regulatory cells (Tregs). These Tregs genetically phenotypically distinct from larger conventional Treg population. For instance, analogous to natural killer (NKT) they have an activated phenotype (CD62Llo, CD44hi) constitutively express IL-10....
Abstract Regulatory T cells (Tregs) play a pivotal role in regulating immune responses and are significant source of the anti-inflammatory cytokine IL-10 intestine. Defects Tregs function lead to development systemic intestinal inflammation. Using single-cell BTB-ZF transcription factor expression analysis, we identified distinct subset Tregs. These have an activated phenotype (CD62Llo, CD44hi) constitutively express Il10. Moreover, these accumulate intestine expand number following DSS...