A5026511947- Wound Healing and Treatments
- Diagnosis and Treatment of Venous Diseases
- Pressure Ulcer Prevention and Management
- Diabetic Foot Ulcer Assessment and Management
- Systemic Sclerosis and Related Diseases
- Dermatologic Treatments and Research
- Mesenchymal stem cell research
- Eosinophilic Disorders and Syndromes
- Surgical Sutures and Adhesives
- Autoimmune Bullous Skin Diseases
- Skin and Cellular Biology Research
- Venous Thromboembolism Diagnosis and Management
- Skin Diseases and Diabetes
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Tendon Structure and Treatment
- Cellular Mechanics and Interactions
- Dermatology and Skin Diseases
- Cell Adhesion Molecules Research
- Nail Diseases and Treatments
- Blood properties and coagulation
- Periodontal Regeneration and Treatments
- Nonmelanoma Skin Cancer Studies
- Dermatological and COVID-19 studies
- Fibroblast Growth Factor Research
Boston University
2016-2025
University School
1998-2024
Dartmouth–Hitchcock Medical Center
2023
University of Insubria
2018
Roger Williams Medical Center
2004-2013
Providence College
2004-2013
Brown University
2013
University Medical Center
2013
Rogers (United States)
2005
SKiN Health
2005
Transforming growth factor type beta (TGF-beta), when injected subcutaneously in newborn mice, causes formation of granulation tissue (induction angiogenesis and activation fibroblasts to produce collagen) at the site injection. These effects occur within 2-3 days dose levels than 1 microgram. Parallel vitro studies show that TGF-beta marked increase either proline or leucine incorporation into collagen an NRK rat fibroblast cell line early passage human dermal fibroblasts. Epidermal (EGF)...
The nonhematopoietic component of bone marrow includes multipotent mesenchymal stem cells (MSC) capable differentiating into fat, bone, muscle, cartilage, and endothelium. In this report, we describe the cell culture characterization, delivery system, successful use topically applied autologous MSC to accelerate healing human experimental murine wounds. A single aspirate 35–50 mL was obtained from patients with acute wounds (n = 5) skin cancer surgery chronic, long-standing, nonhealing lower...
OBJECTIVE— We assessed in a randomized prospective trial the effectiveness of Graftskin, living skin equivalent, treating noninfected nonischemic chronic plantar diabetic foot ulcers. RESEARCH DESIGN AND METHODS— In 24 centers U.S., 208 patients were randomly assigned to ulcer treatment either with Graftskin (112 patients) or saline-moistened gauze (96 patients, control group). Standard state-of-the-art adjunctive therapy, which included extensive surgical debridement and adequate...
<h3>Objective</h3> To test the safety, efficacy, and immunological impact of a cultured allogeneic human skin equivalent (HSE) in treatment venous ulcers. <h3>Design</h3> Prospective, randomized study. <h3>Setting</h3> Multicenter study outpatient setting. <h3>Intervention</h3> Each patient with ulcer received either compression therapy alone or HSE. The patients were evaluated for HSE complete (100%) healing, time to wound closure, recurrence, immune response <h3>Outcome</h3> was completed...
Abstract Bone marrow has long been known to be a source of stem cells capable regeneration the hematopoeitic system. Recent reports, however, have indicated that bone might also contain early can differentiate into other organ tissues such as skin. While these studies illustrated could find their way skin, they not addressed dynamics how participate in homeostatis and In this report we followed green fluorescent protein (GFP) labeled transplanted non‐GFP mice order determine participation...
Older adults are more likely to have chronic wounds than younger people, and the effect of on quality life is particularly profound in this population. Wound healing slows with age, but basic biology underlying influence age‐associated changes wound poorly understood. Most studies used vitro approaches various animal models, observed translate human conditions. The age accompanying multimorbidity effectiveness existing emerging treatment for also unknown, older tend be excluded from...
The incidence of chronic wounds is increased among older adults, and the impact on quality life particularly profound in this population. It well established that wound healing slows with age. However, basic biology underlying influence age-associated changes are poorly understood. Most studies have used vitro approaches various animal models, but observed translate to human conditions. age accompanying multi-morbidity effectiveness existing emerging treatment for also unknown, adults tend...
The efficacy of a bilayered, living skin construct (APLIGRAF(R) [Graftskin]) was evaluated in patients (n = 120) with hard- to-heal venous leg ulcers greater than 1 year's duration. study prospective, randomized, and controlled. Patients received Graftskin plus compression therapy, or standard therapy (active control). were for frequency time to complete (100%) wound closure. Treatment significantly more effective active control the percentage healed by 6 months (47% vs. 19%; p < 0.005)...
It has been proposed that occlusive wound dressings may enhance chronic repair by the stimulatory action of fluid accumulating beneath dressings. In this report, we investigated in vitro proliferative effects obtained from under a polyurethane membrane applied for 24 hours to venous ulcers ambulatory setting. By measuring cell counts and DNA synthesis, found inhibited proliferation human dermal fibroblasts ( p = 0.008) failed stimulate microvascular endothelial cells 0.03) keratinocytes...
One proposed mechanism for the beneficial effect of occlusive dressings on healing is maintenance contact between wound bed and accumulated fluid, which thought to contain growth stimulatory substances. We have examined human fluid in vitro dermal fibroblasts umbilical vein endothelial cells. Acute was collected from six patients undergoing split-thickness skin grafting. The acute sterilely daily underneath a vapor-permeable membrane applied donor site changed every 24 hours 3 days...
A great deal of interest has been focused recently on the potential use synthetic polypeptide growth factors to stimulate healing chronic wounds. In this pilot double-blind randomized study conducted at a single center, we used human recombinant epidermal factor (h-EGF) treat 44 patients with venous ulceration lower extremities. An aqueous solution (10 micrograms/mL) h-EGF was applied topically ulcers twice day until occurred or for maximum 10 weeks. Patients were evaluated weekly...
Abstract Recent findings point to low oxygen tension (hypoxia) as an important mechanism for the expression of several eukaryotic genes. We have previously shown that hypoxia (2% O 2 ), when compared standard (20% upregulates mRNA levels human α1(I) (COL1A1) procollagen gene and transforming growth factor‐beta1 (TGF‐β1) in dermal fibroblasts. In this report, we determined effect on collagen synthesis transcription. Exposure fibroblasts 24–72 h led a threefold, dose‐dependent increase...