High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines diagnostics and therapeutics. Here we report a simple versatile coaxial turbulent jet mixer that can synthesize variety NPs at high throughput up to 3 kg/d, while maintaining the advantages homogeneity, reproducibility, tunability are normally accessible only in specialized microscale mixing devices. The device fabrication does not require...

We report a contraction-expansion array (CEA) microchannel device that performs label-free high-throughput separation of cancer cells from whole blood at low Reynolds number (Re). The CEA microfluidic utilizes hydrodynamic field effect for cell separation, two kinds inertial effects: (1) lift force and (2) Dean flow, which results in size-based with high throughput. To avoid damages potentially caused by shear stress conventional techniques, the isolates operational Re, maintaining using...

We propose a new hydrophoretic method for continuous blood cell separation using microfluidic device composed of slanted obstacles and filtration obstacles. The have larger height gap than the particles in order to focus them sidewall by hydrophoresis. In successive structure, with pore are set between diameters small large particles, which defines critical diameter. Accordingly, smaller criterion freely pass through keep their focused position. contrast, collide against obstacle move into...

Loss of contractility and acquisition an epithelial phenotype vascular smooth muscle cells (VSMCs) are key events in proliferative pathologies such as atherosclerosis post-angioplastic restenosis. There is no proper cell culture system allowing differentiation VSMCs so that it difficult to delineate the molecular mechanism responsible for vasculopathy. We investigated whether a micropatterned substrate could restore contractile vitro. To induce maintain differentiated VSMC vitro, we...

Actin is an essential protein in almost all life forms. It mediates diverse biological functions, ranging from controlling the shape of cells and cell movements to cargo transport formation synaptic connections. Multiple diseases are closely related dysfunction actin or actin-related proteins. Despite importance actin, super-resolution imaging it tissue still challenging, as forms very dense networks inside tissue. In this work, we demonstrate multiplexed volumetric both cultured mouse brain...

Accurately analyzing the functional activities of natural killer (NK) cells in clinical diagnosis remains challenging due to their coupling with other immune effectors. To address this, an integrated cell separator is required, which necessitates a streamlined sample preparation workflow including immunological isolation, removal excess red blood (RBCs), and buffer exchange for downstream analysis. Here, self-powered magneto-microfluidic separation (SMS) chip presented, outputs high-purity...

Blood plasma separation from whole blood is often limited by numerous cells which can compromise processes and thus deteriorate performance such as purity throughput. To address this challenge, we present a microfluidic pipet tip composed of slant array ridges that enable autonomous cell focusing without significant deviation well facilitating high degree parallelization compromising purity. With these advantages, achieved high-purity (99.88%) high-throughput (904.3 μL min-1) blood. In...

Abstract A major challenge to scale up a microfluidic magnetic separator for extracorporeal blood cleansing applications is overcome low drag velocity caused by viscous components interfering with magnetophoresis. Therefore, there an unmet need develop effective method position particles the area of augmented flux density gradients while retaining clinically applicable throughput. Here, magnetophoretic cell separation device, integrated slanted ridge‐arrays in channel, reported. The ridges...

Functional and phenotypic analyses of peripheral white blood cells provide useful clinical information. However, separation from requires a time-consuming, inconvenient process thus separated are limited in settings. To overcome this limitation, microfluidic platform is developed to enable deterministic migration cells, directing the into designated positions according ridge pattern. The uses slant structures on channel top induce migration, which allows efficient high-throughput unprocessed...

An integrated method for blood plasma separation is presented by combining a pneumatic device, which referred to as "smart pipette," and hydrophoretic microchannel microfluidic pipette tip whole-blood sample preparation. This enables hemolysis-free, high-purity through smart pipetting of whole blood, potentially providing the means rapid, inexpensive preparation point-of-care testing. As service our authors readers, this journal provides supporting information supplied authors. Such...

Abstract Rapid prototyping of microfluidic devices has advanced greatly, along with the development 3D printing and micromachining technologies. However, peripheral systems for microfluidics still rely on conventional equipment, such as bench‐top microscopy syringe pumps, which limit system modification further improvements. Herein, optofluidic modular blocks are presented discrete elements to modularize optical fluidic used on‐demand open‐source whole systems. Each block is fabricated by...

We report an improved magnetophoretic method, isomagnetophoresis, employing the magnetic susceptibility gradient across a microfluidic channel applied by field, and we have successfully discriminated polystyrene (PS; 14.78 ± 0.20 μm in diameter), poly(methyl methacrylate) (PMMA; 15.00 0.77 μm) borosilicate (BS; 14.01 1.00 microspheres, where PS PMMA particles similar diamagnetic that cannot be distinguished conventional magnetophoresis. This platform can to label-free discrimination of...

This paper demonstrates a method of modulating hydrophoretic focusing and sorting by inertial forces in microfluidic device. At low Reynolds number (Re), hydrophoresis enables size-based generating specific equilibrium positions for differently sized particles. As increasing Re, becomes coupled with effects that bias the to create narrow stream. Using this method, we achieved both sheathless

This communication presents quantitative studies of the dynamic adhesion behavior mesenchymal stem cells (MSCs) enabled by combination cell-surface receptor-ligand interactions and three-dimensional hydrodynamic control microtopography.

Smart microfluidic pipette tip: A method for removing cells and recovering liquid medium is presented applied blood cell rejection cytotoxicity assay. This enables continuous by manual operation, potentially providing the means rapid, inexpensive sample preparation personalized diagnostics mobile laboratory.

One-step purification of white blood cells (WBCs) is essential to automate sample preparation steps for WBC analysis, but conventional methods such as red cell (RBC) lysis and density-gradient centrifugation typically require harsh chemical or physical treatment, followed by repeated manual washing steps. Alternative microfluidic separation show limited performances due the trade-off between purity throughput. Herein, an integrated device developed decouple synergistically combining a slant...

This paper demonstrates an inertia-activated cell sorting method to separate cells based on their surface protein expression by using inertial microfluidics.