A5023488661- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Methane Hydrates and Related Phenomena
- Microbial Fuel Cells and Bioremediation
- Water Treatment and Disinfection
- Chromium effects and bioremediation
- Genomics and Phylogenetic Studies
- Membrane Separation Technologies
- Adsorption and biosorption for pollutant removal
- Constructed Wetlands for Wastewater Treatment
- Vibrio bacteria research studies
- Geochemistry and Elemental Analysis
- Fecal contamination and water quality
- Risk and Safety Analysis
- Aquaculture disease management and microbiota
- Environmental remediation with nanomaterials
- Bacteriophages and microbial interactions
- Electric and Hybrid Vehicle Technologies
- Protist diversity and phylogeny
- Microbial Applications in Construction Materials
- Engine and Fuel Emissions
- Advanced Power Generation Technologies
- Plant Pathogenic Bacteria Studies
- transportation and logistics systems
National Institute for Environmental Studies
2011-2024
National College of Technology, Wakayama College
2018-2022
National Institute of Technology
2018-2022
Nagaoka University of Technology
2011-2015
Japan Agency for Marine-Earth Science and Technology
2014-2015
Nagaoka University
2014
Nagaoka National College of Technology
2011
United States Government Accountability Office
2011
Anaerobic oxidation of methane (AOM) in marine sediments is an important global sink, but the physiological characteristics AOM-associated microorganisms remain poorly understood. Here we report cultivation AOM microbial community from deep-sea methane-seep sediment using a continuous-flow bioreactor with polyurethane sponges, called down-flow hanging sponge (DHS) bioreactor. We anaerobically incubated collected Nankai Trough, Japan, for 2,013 days at 10°C. Following incubation, active...
Water quality parameters influence the abundance of pathogenic bacteria. The genera Aeromonas, Arcobacter, Klebsiella, and Mycobacterium are among representative bacteria identified in wastewater. However, information on correlations between water these bacteria, as well their reduction rate existing wastewater treatment facilities (WTFs), is lacking. Hence, this study aimed to determine rates bacterial groups WTFs. Sixty-eight samples (34 influent 34 non-disinfected, treated, effluent...
A down-flow hanging sponge reactor, constructed by connecting three identical units in series, was applied to the treatment of artificial wastewater containing phenol and ammonia under high salinity conditions (10.9 g-Cl(-)/L). The theoretical hydraulic retention time (HRT) each unit 4 h (total HRT = 12 h). To enhance denitrification effluent recirculation, recirculation ratio increased increments ranging from 0.0 2.0. concentration total nitrogen (TAN), NO2-N, NO3-N final as a proportion...
Our study aimed to assess the effect of enriched sludge and paddy soil as inocula on autotrophic nitrogen removal using hollow fiber membrane reactors. Mixed from parent reactors were inoculated into two separate upflow anaerobic reactors, UR1 UR2, respectively. Each reactor was constructed a plastic cylinder equipped with U-shaped gas diffuser. These operated for 240 days compare their performance under both non-specialized specialized conditions favoring either anammox, nitrite...
Markedly diverse sequences of the adenosine-5'-phosphosulfate reductase alpha subunit gene (aprA), which encodes a key enzyme in microbial sulfate reduction and sulfur oxidation, were detected subseafloor sediments on northwestern Pacific off Japan. The aprA grouped into 135 operational taxonomic units (90% sequence identity), including genes related to putative sulfur-oxidizing bacteria predominantly sulfate-depleted deep sediments. Our results suggest that ecosystems biosphere have...
The method of spiking synthetic internal standard genes (ISGs) to samples for amplicon sequencing, generating sequences and converting absolute gene numbers from read counts has been used only phylogenetic markers not applied functional markers. In this study, we developed ISGs, including the 16S rRNA, pmoA, encoding a subunit particulate methane monooxygenase amoA, ammonia monooxygenase. We added ISGs samples, amplified target performed sequencing. For mock community, copy converted using...
To find taxonomically novel methanogenic archaea, a clone analysis targeting mcrA gene (a functional molecular marker of archaea) was conducted for four anaerobic granular sludges. Several phylotypes were clearly different from those other groups known methanogens. These found to belong an unidentified group called as MCR-2b group. Comparative phylogenetic deduced amino acid McrA and 16S rRNA sequences revealed that the MCR2-b is possibly derived methanogen group; have been classified into...
Ureolysis-driven microbially induced carbonate precipitation (MICP) has recently received attention for its potential biotechnological applications. However, information on the enrichment and production of ureolytic microbes by using bioreactor systems is limited. Here, we report a low-tech down-flow hanging sponge (DHS) system microbes. Using this yeast extract-based medium containing 0.17 M urea, with high urease activity (> 10 μmol urea hydrolyzed per min ml culture) were repeatedly...
Real-time PCR (polymerase chain reaction) 定量でスタンダードDNAとして用いるPCR増幅産物の適合性を,Real-time PCR法により作成した検量線により評価した。同じ遺伝子コピー数におけるThreshold cycle(Ct値)は,メタン生成古細菌のゲノムDNAとその16S rRNA遺伝子を増幅したPCR増幅産物で-0.7~5.6サイクル異なった。この結果は,スタンダードDNAにPCR増幅産物を用いる場合,定量誤差が発生することを示すものである。また,検量線の傾きに基づいたPCR増幅効率(E)は,PCR増幅産物のReal-time PCR定量におけるスタンダードDNAとしての適合性を評価するには不十分であった。
Given the toxicity and widespread occurrence of hexavalent chromium [Cr(VI)] in aquatic environments, we investigated feasibility a down-flow hanging sponge (DHS) biofilm reactor for enrichment microbial communities capable Cr(VI) removal. In present study, laboratory-scale DHS fed with molasses-based medium containing was operated 112 days investigation. The Cr(VI)-removing evaluated based on water quality prokaryotic community analyses. Once began to operate, high average volumetric...
Geologic and anthropogenic activities have led to increased heavy metal contamination in soils groundwater.This paper describes a newly isolated aerobic denitrifying bacterial strain, WKDN, with the capacity for calcium carbonate (CaCO 3 ) biomineralization, evaluates its potential use dissolved zinc (Zn) removal.Our 16S rRNA gene-based phylogenetic analysis assigned strain Acinetobacter genus.An vitro experiment indicated that g acetate-carbon/g nitrate-nitrogen ratio of biomineralization...
Down-flow hanging sponge (DHS) reactors, employed in domestic wastewater treatment, have demonstrated efficacy eliminating Escherichia coli and other potentially pathogenic bacteria. The aim of this study was to elucidate the mechanism removal E. by employing a cube-shaped polyurethane carrier within compact reactor. An experiment conducted on prepared sponge. Escherichia. level found decrease more than 2 logs after passing through five nutrient-restricted DHS sponges. Conversely, newly...
ABSTRACT Anaerobic treatment of sulfur-rich wastewater is challenging because sulfide greatly inhibits the activity anaerobic microorganisms, especially methanogenic archaea. We developed an internal phase-separated reactor (IPSR) that removed prior to methanogenesis by gas stripping using biogas produced in reactor. The IPSR was fed with synthetic containing a very high concentration up 6,000 mg S L−1 chemical oxygen demand (COD) 30,000 L−1. operated at organic loading rate 5–12 kg COD m−3...