A5077269954- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Ubiquitin and proteasome pathways
- Lung Cancer Treatments and Mutations
- Cytokine Signaling Pathways and Interactions
- HER2/EGFR in Cancer Research
- Parasites and Host Interactions
- Parasitic Infections and Diagnostics
- S100 Proteins and Annexins
- Trypanosoma species research and implications
- Electronic Health Records Systems
- Protein Degradation and Inhibitors
- Retinoids in leukemia and cellular processes
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Meta-analysis and systematic reviews
- Virus-based gene therapy research
- Ethics in Clinical Research
- Animal Virus Infections Studies
- Cancer-related Molecular Pathways
- Bioinformatics and Genomic Networks
- SARS-CoV-2 and COVID-19 Research
- Parasitic infections in humans and animals
- Metabolomics and Mass Spectrometry Studies
University of Arkansas for Medical Sciences
2020-2023
Washington University in St. Louis
2021
Baylor College of Medicine
2018-2020
Texas Children's Hospital
2018
Abstract Expression of the serine/threonine kinase never in mitosis gene A (NIMA)–related 2 (NEK2) is essential for entry into via its role facilitating centrosome separation. Its overactivity can lead to tumorigenesis and drug resistance through activation several oncogenic pathways, including AKT. Although cancer-enabling activities NEK2 are documented many malignancies, correlations with poor survival myeloma, breast, non–small cell lung cancer, little known about lymphoma. Here, tumors...
Quantitative proteomics generates large datasets with increasing depth and quantitative information.
To determine whether the presence of Blastocystis is associated with other gastrointestinal parasite infections, stool samples from 95 Honduran rural children were analyzed using multi-parallel quantitative real-time polymerase chain reaction (PCR) and Kato–Katz. Combined results detected following prevalence: Blastocystis, 71.6%; Trichuris trichiura, 63.2%; Giardia lamblia, 40.0%; Ascaris lumbricoides, 15.8%; Necator americanus, 4.2%. Age was found quantity both DNA (rs = 0.524, P < 0.001)...
Parasitic infections are likely under-recognized among immigrant populations in the USA. We conducted a cross-sectional study to evaluate if such have health impacts recent immigrants Chicago and identify predictive factors for parasitic infections. A total of 133 were enrolled, filling out standardized medical questionnaire providing blood stool samples. Appriximately 12% subjects (15/125) who provided or sample testing found evidence current prior infection with pathogenic parasite, which...
<p>NBI-961 causes loss of NEK2 and phosphorylation. A, B, Western blot analysis phosphorylated (p-NEK2) protein expression in NBI-961 treated RIVA (A) U2932 (B) cell lines after 24 h. Quantification band intensity normalized to ACTIN as mean ± SEM from three independent experiments shown a bar graph below each blot. Adjusted P-values determined by one-way ANOVA with Dunnett’s multiple test correction for comparisons DMSO vehicle control (VTC). *P < 0.05, **P 0.01, ***P 0.001, ****P...
<p>Original blot images from Figure 5H in the main text. Uncropped of 5H. Samples were run on duplicate gels and each was immunoblotted with either a mixture anti-NEK2 anti-ACTIN primary antibodies (left panels) or anti-p-AKT, anti-AKT, (right panels). The blots imaged both Dylight 650 800 channels shown as respective image (NEK2 p-AKT top panels, AKT ACTIN lower All resolved secondary using BioRad ChemiDoc MP described methods.</p>
<p>Differentially expressed proteins identified from the total proteomic analysis in SUDHL5 and RIVA treated with NBI-961 relative to DMSO vehicle control using log2 fold change greater than or equal 1 less -1.</p>
<p>KEA3 analysis that used the Cheng kinase-substrate interaction network in SUDHL5 and RIVA treated with NBI-961 relative to DMSO vehicle control using top 25 proteins from each a signficant loss of phosphorylation (FDR < 0.05; log2 fold change less than or equal -1).</p>
<p>Short Tandem Repeat (STR) analysis of the DLBCL and benign B cell lines.</p>
<p>Short Tandem Repeat (STR) analysis of the DLBCL and benign B cell lines.</p>
<p>Cox proportional hazard model of patients with DLBCL adjusted for prognostic factors.</p>
<p>Differentially expressed proteins identified from the phospho-proteomic analysis in RIVA treated with NBI-961 relative to DMSO vehicle control.</p>
<p>Original blot images from Figure 4 in the main text. Uncropped of (A) 4A; (B) 4C; (C) 4G; and (D) 4H. Each was imaged both Dylight 650 800 channels shown as respective image (NEK2 top panels ACTIN lower panels). All blots were immunoblotted with a mixture anti-NEK2 anti-ACTIN primary antibodies, resolved secondary antibodies using BioRad ChemiDoc MP described methods.</p>
<p>Original blot images from Supplementary Figure S9. Uncropped of (A) S9A and (B) S9B. The in also contained samples a replicate the SUDHL5 untreated cell lysate that was incubated without with phosphatase an additional molecular weight ladder to separate two sample sets. Each imaged both Dylight 650 800 channels shown as respective image (NEK2 top panels ACTIN lower panels). All blots were immunoblotted mixture anti-NEK2 anti-ACTIN primary antibodies, resolved secondary antibodies...
<p>Differentially expressed proteins identified from the total proteomic analysis in RIVA treated with NBI-961 relative to DMSO vehicle control.</p>
<p>Supplemental Methods: Gene Expression by Quantitative PCR</p>
<p>Synthesis of NBI-961. Reagents and Conditions: a) NaOMe, MeOH 0 °C – rt. b) PPh3, DIAD, DCM - c) LDA, dry THF, -78 d) Na2CO3, Pd(dppf)Cl2.DCM, 4:1 THF:H2O. e) 7M NH3 in MeOH, NH4OH, 78 °C. f) K2CO3, DMF, 80 °C.</p>
<p>DLBCL patient characteristics.</p>
<p>DLBCL patient characteristics.</p>
<p>Differentially expressed proteins identified from the total proteomic analysis in SUDHL5 relative to VAL DLBCL cells (untreated).</p>
<div>Abstract<p>Expression of the serine/threonine kinase never in mitosis gene A (NIMA)–related 2 (NEK2) is essential for entry into via its role facilitating centrosome separation. Its overactivity can lead to tumorigenesis and drug resistance through activation several oncogenic pathways, including AKT. Although cancer-enabling activities NEK2 are documented many malignancies, correlations with poor survival myeloma, breast, non–small cell lung cancer, little known about...
<p>Original blot images from Figure 5H in the main text. Uncropped of 5H. Samples were run on duplicate gels and each was immunoblotted with either a mixture anti-NEK2 anti-ACTIN primary antibodies (left panels) or anti-p-AKT, anti-AKT, (right panels). The blots imaged both Dylight 650 800 channels shown as respective image (NEK2 p-AKT top panels, AKT ACTIN lower All resolved secondary using BioRad ChemiDoc MP described methods.</p>
<p>Differentially expressed proteins identified from the phospho-proteomic analysis in SUDHL5 treated with NBI-961 relative to DMSO vehicle control.</p>
<p>Supplemental Methods: Gene Expression by Quantitative PCR</p>