A5024815626- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Ubiquitin and proteasome pathways
- Lung Cancer Treatments and Mutations
- Cytokine Signaling Pathways and Interactions
- Analytical chemistry methods development
- HER2/EGFR in Cancer Research
- Mass Spectrometry Techniques and Applications
- Colorectal and Anal Carcinomas
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Radioactive element chemistry and processing
- Cancer-related Molecular Pathways
- Electrochemical Analysis and Applications
- Genetic factors in colorectal cancer
- Analytical Chemistry and Chromatography
- S100 Proteins and Annexins
- Chronic Kidney Disease and Diabetes
- Protein Degradation and Inhibitors
- Mercury impact and mitigation studies
- Colorectal Cancer Treatments and Studies
- Multiple and Secondary Primary Cancers
- Coordination Chemistry and Organometallics
University of Arkansas for Medical Sciences
2021-2024
Mississippi State University
2020
Kathmandu University
2017-2020
B.P. Koirala Memorial Cancer Hospital
2010
Sri Venkateswara University
1985
Abstract Expression of the serine/threonine kinase never in mitosis gene A (NIMA)–related 2 (NEK2) is essential for entry into via its role facilitating centrosome separation. Its overactivity can lead to tumorigenesis and drug resistance through activation several oncogenic pathways, including AKT. Although cancer-enabling activities NEK2 are documented many malignancies, correlations with poor survival myeloma, breast, non–small cell lung cancer, little known about lymphoma. Here, tumors...
Breast cancer is one of the common cancers. Hormonal therapy along with surgery, chemotherapy, radiotherapy and targeted are vital modalities for management breast cancer. Tamoxifen has been most widely used hormonal more than two decades. In this article we review benefits, dose, duration timing in patients cancer.
 Keywords: cancer, therapy, tamoxifen.
Mass spectrometers are ever-increasingly powerful, user-friendly, and affordable. Thus, the addition of mass spectrometry experiments into undergraduate laboratory curriculum is now both feasible an effective tool to introduce students relevant instrumentation. Here experiment demonstrating use a high-resolution electrospray ionization spectrometer (ESI-HRMS) for identification peptide sequences differentiation isomeric peptides was developed implemented in biochemistry course third-year...
A radiochemical displacement method for the determination of micro amounts Hg (II) has been developed. 65Zn was displaced from Zn-1- (2 pyridylazo-2-naphthol) complex at pH 5 in borate buffers by . 10-80 μg mercury could be determined. Interference various metal ions and methods suppression have also carried out.
Hydroxyproline is a common variation of proline, with diverse biological roles. The hydroxylation proline gives rise to several (natural and/or synthetic) isomeric forms, including both positional isomers and stereoisomers. While mass spectrometry widely touted as very selective analytical technique, the identification closely related often poses challenge. In these cases, allied technologies become helpful in providing full characterization. Here, infrared multiple photon dissociation...
<b>Abstract ID 95401</b> <b>Poster Board 482</b> <b>Objectives/Goals:</b> Renal fibrosis is an outcome of chronic kidney disease that affects approximately one-tenth the global community. Its pathogenesis and progression are highly multi-factorial, likely demanding a need to target various mechanisms for providing better treatment. Using animal model renal fibrosis, we sought test therapeutic response TK-850, experimental drug acts as dual-inhibitor transforming growth factor beta receptor 1...
<p>NBI-961 causes loss of NEK2 and phosphorylation. A, B, Western blot analysis phosphorylated (p-NEK2) protein expression in NBI-961 treated RIVA (A) U2932 (B) cell lines after 24 h. Quantification band intensity normalized to ACTIN as mean ± SEM from three independent experiments shown a bar graph below each blot. Adjusted P-values determined by one-way ANOVA with Dunnett’s multiple test correction for comparisons DMSO vehicle control (VTC). *P < 0.05, **P 0.01, ***P 0.001, ****P...
<p>Original blot images from Figure 5H in the main text. Uncropped of 5H. Samples were run on duplicate gels and each was immunoblotted with either a mixture anti-NEK2 anti-ACTIN primary antibodies (left panels) or anti-p-AKT, anti-AKT, (right panels). The blots imaged both Dylight 650 800 channels shown as respective image (NEK2 p-AKT top panels, AKT ACTIN lower All resolved secondary using BioRad ChemiDoc MP described methods.</p>
<p>Differentially expressed proteins identified from the total proteomic analysis in SUDHL5 and RIVA treated with NBI-961 relative to DMSO vehicle control using log2 fold change greater than or equal 1 less -1.</p>
<p>KEA3 analysis that used the Cheng kinase-substrate interaction network in SUDHL5 and RIVA treated with NBI-961 relative to DMSO vehicle control using top 25 proteins from each a signficant loss of phosphorylation (FDR < 0.05; log2 fold change less than or equal -1).</p>
<p>Short Tandem Repeat (STR) analysis of the DLBCL and benign B cell lines.</p>
<p>Short Tandem Repeat (STR) analysis of the DLBCL and benign B cell lines.</p>
<p>Cox proportional hazard model of patients with DLBCL adjusted for prognostic factors.</p>
<p>Differentially expressed proteins identified from the phospho-proteomic analysis in RIVA treated with NBI-961 relative to DMSO vehicle control.</p>
<p>Original blot images from Figure 4 in the main text. Uncropped of (A) 4A; (B) 4C; (C) 4G; and (D) 4H. Each was imaged both Dylight 650 800 channels shown as respective image (NEK2 top panels ACTIN lower panels). All blots were immunoblotted with a mixture anti-NEK2 anti-ACTIN primary antibodies, resolved secondary antibodies using BioRad ChemiDoc MP described methods.</p>
<p>Original blot images from Supplementary Figure S9. Uncropped of (A) S9A and (B) S9B. The in also contained samples a replicate the SUDHL5 untreated cell lysate that was incubated without with phosphatase an additional molecular weight ladder to separate two sample sets. Each imaged both Dylight 650 800 channels shown as respective image (NEK2 top panels ACTIN lower panels). All blots were immunoblotted mixture anti-NEK2 anti-ACTIN primary antibodies, resolved secondary antibodies...
<p>Differentially expressed proteins identified from the total proteomic analysis in RIVA treated with NBI-961 relative to DMSO vehicle control.</p>
<p>Supplemental Methods: Gene Expression by Quantitative PCR</p>