Emissive f-block coordination complexes constitute an important class of optical probes, with applications ranging from sensing bioactive species, high throughput assays and screening protocols in vitro, to time-resolved imaging studies cellulo or vivo. The key chemistry issues be addressed complex design characterisation are defined, emphasis on the use emissive europium terbium their conjugates molecular imaging. Both luminescent 'tags' useful energy transfer 'responsive' systems for discussed.

Every nucleolus has a silver lining: Formation of nanoclusters by fluorescence photoactivation was used for the staining cells at low nitrate concentrations and ambient temperature. The picture shows picosecond lifetime images peptide-encapsulated within NIH 3T3 cells. Supporting information this article is available on WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z604253_s.pdf or from author. Please note: publisher not responsible content functionality any supporting supplied...

Clustering together: A shuttle-based fluorogenic cluster transfer, in which silver clusters (SCs) are directly transferred from a low-molecular-weight poly(acrylic acid) (PA) shuttle to single-stranded DNA (ssDNA) tag on the protein of interest (see picture), proceeds with excellent specificity. Upon fluorescence increases more than ten times, provides bright, photostable labeling. Detailed facts importance specialist readers published as ”Supporting Information”. Such documents...

A europium complex selectively staining the nucleolus of NIH 3T3, HeLa, and HDF cells is reported. This possesses not only advantage long lifetime emission (0.3 ms), but also a chromophore that allows excitation at relatively wavelength (λmax = 384 nm) gives rise to an acceptable quantum yield (9%). The can be used both in live cell fixed imaging, giving average intracellular concentration on order 0.5 μM. Strong binding serum albumin has been demonstrated by examination analogous gadolinium...

Abstract DNA‐encapsulated silver clusters are readily conjugated to proteins and serve as alternatives organic dyes semiconductor quantum dots. Stable bright on the bulk single molecule levels, Ag nanocluster fluorescence is observed when staining live cell surfaces. Being significantly brighter more photostable than organics much smaller dots with a point of attachment, these nanomaterials offer promising new approaches for biolabeling.

Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation demonstrated high-background environments extract weak signals from...

The stability of silica nanostructure in the core-silica shell nanomaterials is critical to understanding activity these since exposure core materials due poor may cause misinterpretation experiments, but unfortunately reports on have been inconsistent. Here, we show that luminescent silver nanodots (AgNDs) can be used monitor nanostructures. Though relatively stable water and phosphate buffered saline, nanoparticles are eroded by biological media, leading AgNDs from AgND@SiO2 quenching...

Recently, various studies related to the photophysical properties of nanoscale and subnanoscale particles have been actively carried out in chemical biological fields. However, terminology these nanoparticles has not clearly defined, which causes confusion among research groups students. This article aims clarify definitions four terms: quantum dots (QDs), nanodots (NDs), nanoclusters (NCs), clusters. The historical usage terms trends over last five years will be presented. On basis size...

A chemoselective sensor for the citrate anion has been devised, based on a new europium complex that offers ratiometric analysis of long-lived emission.

Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible near-IR emitters. A model indicated that ethylenediamine a secondary amine in the molecule were key components for formation of emissive species. The mechanism enables us control generation elimination

Nucleoli mit Silberstreifen: Die Bildung von Silbernanoclustern durch Fluoreszenz-Photoaktivierung wurde für das Anfärben Zellen bei niedriger Silbernitratkonzentration und Raumtemperatur genutzt. Das Bild zeigt Zerfall in Peptiden eingeschlossenen NIH 3T3-Zellen.

The overproduction of hydrogen peroxide is implicated in the progress numerous life-threatening diseases and there a great need for development contrast agents that can detect vivo. In this communication, we present new agent peroxide, termed peroxalate micelles, which through chemiluminescence, have physical/chemical properties needed vivo imaging applications. micelles are composed amphiphilic based copolymers fluorescent dye rubrene, they 'stealth' polyethylene glycol (PEG) corona to...

Though borohydride has been frequently used as a hydrogen source in the nanoparticle-catalyzed chemical reduction, explanation for delay of reaction is still under debate. Here we investigated mechanism catalytic reduction and found that negative charge prevented it from approaching negatively charged nanoparticles, resulting reactions. Spontaneous hydrolysis was critical to start reaction. A universal method introduced dramatically increase efficiency. Our experiment suggested guideline...

Several azaxanthone and azathioxanthone sensitising chromophores have been incorporated into macrocyclic ligands form well-defined Eu Tb complexes in polar media. Excitation of the heterocyclic chromophore range 330 to 382 nm leads modest amounts aromatic fluorescence relatively efficient metal-based luminescence, with absolute quantum yields up 24% aqueous

The electron spin resonance spectrum of superoxide anion radical adsorbed on the surface colloidal TiO2 was detected directly at room temperature for first time. This signal generated partially by porphyrin-sensitized titanium dioxide, i.e., reduction oxygen nanocluster just injected from excited porphyrin.

Leuchtender Cluster: Die direkte Übertragung fluorogener Silbercluster (SCs) von einer niedermolekularen Polyacrylsäure (PA) als Shuttle auf Einzelstrang-DNA(ssDNA)-Markierungen dem interessierenden Protein (siehe Bild) verläuft hochspezifisch. Bei der nimmt die Clusterfluoreszenz um mehr das Zehnfache zu, was ein helles, photostabiles Markieren ermöglicht. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not...

Reactive oxygen species selectively accelerated transitions between various silver nanodots. The blue was developed as an oxidant-resistant imaging agent and analyte reporter. In addition to the spectral response of nanodots ROS, were formulated detect analytes with excellent selectivity picomolar detection limit when coupled glucose oxidase.

We have developed a colorimetric and fluorescent dual-response probe for the detection of paraquat diquat in aqueous solutions based on an anionic polythiophene derivative. The limit this approach can be as low 10−9 M by fluorescence measurements.

The unique behavior of a new Ru(II) diimine complex, Ru(bpy)(2)(L)(2+) (where L is 4-methyl-4'-[p-(dimethyl- amino)-alpha-styryl]-2,2'-bipyridine, bpy 2,2'-bipyridine), was studied in detail. Due to the strong electron donating property amino group, an ILCT (intraligand charge transfer) state involved either absorption spectra or time-resolved emission spectra. Dual based on (3)MLCT and (3)ILCT states observed at room temperature for first time via technique complexes.