A5075488552- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced MRI Techniques and Applications
- Optical Imaging and Spectroscopy Techniques
- Intracerebral and Subarachnoid Hemorrhage Research
- Biosensors and Analytical Detection
- Neonatal and fetal brain pathology
- Mitochondrial Function and Pathology
- Atomic and Subatomic Physics Research
- Neurosurgical Procedures and Complications
- S100 Proteins and Annexins
- Spectroscopy Techniques in Biomedical and Chemical Research
- Diabetes and associated disorders
- Analytical chemistry methods development
- Advanced Glycation End Products research
- Head and Neck Surgical Oncology
- Antibiotics Pharmacokinetics and Efficacy
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Traumatic Brain Injury Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Spinal Cord Injury Research
- Glycosylation and Glycoproteins Research
- Sepsis Diagnosis and Treatment
University of Cambridge
2017-2024
University of Bedfordshire
2024
A proof-of-concept aptamer-based optical assay is described for the determination of immuno signalling molecule interleukin-6 (IL-6), a key marker acute inflammation. The based on aggregation gold nanoparticles (AuNP) coated in two complimentary "sandwich-style" aptamers, each with different IL-6 target moieties. will recognise aptamer pair and bind to it, thereby causing corresponding functionalised nanoparticles. AuNPs after exposure induces visible colour change from red pink, absorption...
Abstract A key pathophysiological process and therapeutic target in the critical early post-injury period of traumatic brain injury (TBI) is cell mitochondrial dysfunction; characterised by elevation lactate/pyruvate (L/P) ratio absence hypoxia. We previously showed that succinate can improve extracellular chemistry acute TBI, but it was not clear if this translates to a change downstream energy metabolism. studied effect microdialysis-delivered on state (phosphocreatine/ATP (PCr/ATP)) with...
Mitochondrial dysfunction, the inability to efficiently utilise metabolic fuels and oxygen, contributes pathological changes following traumatic spinal cord or brain injury (TBI). In present study, we tested hypothesis that succinate supplementation can improve cellular energy state under metabolically stressed conditions in a robust, reductionist vitro model of mitochondrial dysfunction which primary mixed glial cultures (astrocytes, microglia oligodendrocytes) were exposed complex I...
Metabolic dysfunction is a key pathophysiological process in the acute phase of traumatic brain injury (TBI). Although changes glucose metabolism and extracellular lactate/pyruvate ratio are well known, it was hitherto unknown whether these translate to downstream ATP intracellular pH. We have performed first clinical voxel-based vivo phosphorus magnetic resonance spectroscopy (31P MRS) 13 acute-phase major TBI patients versus 10 healthy controls (HCs), at 3T, focusing on eight central 2.5 ×...
Neuroinflammation has been shown to mediate the pathophysiological response following traumatic brain injury (TBI). Accumulating evidence implicates astrocytes as key immune cells within central nervous system (CNS), displaying both pro- and anti-inflammatory properties. The aim of this study was investigate how in vitro human astrocyte cultures respond cytokines across a concentration range that approximates aftermath TBI. To end, enriched induced pluripotent stem cell (iPSC)-derived were...
The brains of patients suffering from traumatic brain-injury (TBI) undergo dynamic chemical changes in the days following initial trauma. Accurate and timely monitoring these is paramount importance for improved patient outcome. Conventional brain-chemistry performed off-line by collecting manually transferring microdialysis samples to an enzymatic colorimetric bedside analyzer every hour, which detects quantifies molecules interest. However, off-line, hourly means that any subhourly...
Over 500 million people worldwide are affected by diabetes mellitus, a chronic disease that leads to high blood glucose levels and causes severe side effects. The predominant biological marker for diagnosis of is glycated haemoglobin (GHb). In human the reducing sugar, glucose, irreversibly conjugates onto accessible amine groups within Hb. Most methods monitoring selectively detect N-terminal glycation at Val-1 on β-globin chain, but not other sites. Detection epitopes GHb has potential...
Chronic subdural hematoma (CSDH) is a collection of blood and fluid that arises on the brain surface due to combination trauma and/or inflammation. The mainstay treatment surgical drainage, but CSDH can recur. Dexamethasone has been shown reduce recurrence, its mechanism action not fully elucidated. Understanding inflammatory mediators driving formation recurrence how dexamethasone alters this help develop new therapeutic strategies.A subgroup adult patients recruited Dex-CSDH trial,...
Cerebral microdialysis is a sampling technique which offers much potential for understanding inflammatory pathophysiology following traumatic brain injury (TBI). At present, the recovery of cytokines via in clinical studies not straightforward primarily due to their size, steric properties and low concentrations. Heparin heparin-coated microspheres have previously shown promise as cytokine-binding agents enhanced animal models (Duo Stenken Anal Bioanal Chem 399(2):773-82, 2011;...
Cerebral microdialysis (CMD) is used in severe traumatic brain injury (TBI) order to recover metabolites extracellular fluid (ECF). To larger proteins and avoid loss, albumin supplemented perfusion (PF) has been utilized, but because of regulatory changes the European Union, this no longer practicable. The aim with study was see whether fluid, absolute (AR), relative (RR) recovery for novel carrier, Dextran 500, better than conventional PF a range cytokines chemokines. An vitro setup...
How to optimise glucose metabolism in the traumatised human brain remains unclear, including whether injured can metabolise additional when supplied. We studied effect of microdialysis-delivered 1,2- 13 C 2 at 4 and 8 mmol/L on extracellular chemistry using bedside ISCUS flex, fate label group high-resolution NMR recovered microdialysates, 20 patients. Compared with unsupplemented perfusion, increased concentrations pyruvate (17%, p = 0.04) lactate (19%, 0.01), a small increase...
Rationale and objectives Cerebral microdialysis is a technique that enables monitoring of the neurochemistry patients with significant acquired brain injury, such as traumatic injury (TBI) subarachnoid haemorrhage (SAH). can also be used to characterise neuro-pharmacokinetics small-molecule study substrates using retrodialysis/retromicrodialysis. However, challenges remain: (i) lack simple, stable, inexpensive tissue model for drug neuropharmacology; (ii) it unclear how far small...
Cerebral microdialysis (CMD) catheters allow continuous monitoring of patients' cerebral metabolism in severe traumatic brain injury (TBI). The consist a terminal semi-permeable membrane that is inserted into the brain's interstitium to perfusion fluid equalize with surrounding extracellular environment before being recovered through central non-porous channel. However, it unclear how far and suspended metabolites have diffused from within brain, therefore what volume or region tissue...
The brains of patients suffering from traumatic brain-injury (TBI) undergo dynamic chemical changes in the days following initial trauma. Accurate and timely monitoring these is paramount importance for improved patient outcome. Conventional brain-chemistry performed off-line by collecting manually transferring microdialysis samples to an enzymatic colorimetric bedside analyzer every hour, which detects quantifies molecules interest. However, off-line, hourly means that any subhourly...