A5062507586- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Health and Medical Research Impacts
- Health Sciences Research and Education
- Plant Reproductive Biology
- Innovations in Medical Education
Oakland University
2024
University of Colorado Denver
2002-2015
University of Colorado Health
2004-2007
University of Colorado Anschutz Medical Campus
2004-2007
Massachusetts Institute of Technology
2005
Center for Cancer Research
2005
Whitehead Institute for Biomedical Research
2005
Howard Hughes Medical Institute
1999-2005
University of Virginia
1990-2002
Department of Embryology
1999
In eukaryotic cells, cohesin holds sister chromatids together until they separate into daughter cells during mitosis. We have used chromatin immunoprecipitation coupled with microarray analysis (ChIP chip) to produce a genome-wide description of binding meiotic and mitotic chromosomes Saccharomyces cerevisiae. A computer program, PeakFinder, enables flexible, automated identification annotation peaks in ChIP chip data. Cohesin sites are highly conserved meiosis mitosis, suggesting that share...
The nucleosome is the fundamental unit of assembly chromosome and reversible modifications histones have been suggested to be important in many aspects function. structure-function relations amino-terminal domain yeast histone H4 were examined by creation directed point mutations. four lysines subject acetylation essential for function as substitution arginine or asparagine at these positions was lethal. No single lysine residue completely since substitutions each position viable, although...
The normal progression of Saccharomyces cerevisiae through nuclear division requires the function amino-terminal domain histone H4. Mutations that delete domain, or alter 4 conserved lysine residues within cause a marked delay during G2+M phases cell cycle. Site-directed mutagenesis single and multiple failed to map this phenotype any particular site; defect was only observed when all four lysines were mutated. Starting with quadruple lysine-to-glutamine substitution allele, insertion...
The recruitment of cohesins to pericentric chromatin in some organisms appears require heterochromatin associated with repetitive DNA. However, neocentromeres and budding yeast centromeres lack flanking DNA, indicating that cohesin occurs through an alternative pathway. Here, we demonstrate all chromosomes assemble domains extend over 20-50 kb unique sequences the conserved 120-bp centromeric assembly these requires presence a functional kinetochore every cell cycle. A similar enhancement...
Sister chromatid cohesion, conferred by the evolutionarily conserved cohesin complex, is essential for proper chromosome segregation. Cohesin binds to discrete sites along arms, and especially enriched surrounding centromeres, but past studies have not clearly defined roles of arm pericentromeric cohesion in To address this issue, we developed a technique that specifically reduced association on single without affecting binding. Under these conditions, observed more extensive stretching...
The stepwise loss of cohesins, the complexes that hold sister chromatids together, is required for faithful meiotic chromosome segregation. Cohesins are removed from arms during meiosis I but maintained around centromeres until II. Here we show Sgo1, a protein protecting centromeric cohesins removal I, localizes to cohesin-associated regions (CARs) at centromere and 50-kb region surrounding it. Establishment this Sgo1-binding domain requires 120-base-pair (bp) core centromere, kinetochore...
Cohesins mediate sister chromatid cohesion and DNA repair also function in gene regulation. Chromosomal cohesins are distributed nonrandomly, their deposition requires the heterodimeric Scc2/Scc4 loader. Whether establishes nonrandom cohesin distributions on chromosomes is poorly characterized, however. To better understand spatial regulation of association, we mapped budding yeast Scc2 Scc4 chromosomal distributions. We find that resides at previously cohesin-associated regions (CARs)...
AbstractThe histone proteins are essential for the assembly and function of eukaryotic chromosome. Here we report first isolation a temperature-sensitive lethal H4 mutant defective in mitotic chromosome transmission Saccharomyces cerevisiae. The requires two amino acid substitutions H4: Thr-to-Ile change at position 82, which lies within one DNA-binding surfaces protein, substitution Ala to Val 89 that is an intragenic suppressor. Genetic biochemical evidence shows temperature sensitive but...
Cohesion of sister chromatids occurs along the entire length chromosomes, including centromere where it plays essential roles in chromosome segregation. Here, minichromosomes budding yeast Saccharomyces cerevisiae are exploited to generate a functional assay for DNA sequences involved cohesion. The centromeric element CDEIII was found be necessary but not sufficient This shown previously required assembly kinetochore, centromere-associated protein complex that attaches chromosomes spindle....
OPEN ACCESSApril 9, 2024Foundational and Clinical Science Integration in a Team-Based Learning Module Modeling Care of Patient With Dyslipidemia Paul C. Megee, PhD, Virginia Uhley, RDN, James Grogan, Alan Silverman, DO PhD https://orcid.org/0000-0002-9397-2161 Associate Professor, Department Foundational Medical Studies, Oakland University William Beaumont School Medicine E-mail Address: [email protected] , RDN Assistant Internal Medicine, Medicine; Wayne State...
Significance Cohesin complexes tether replicated sister chromatids together from DNA replication until anaphase onset. This tethering promotes proper chromosome segregation, damage repair, and the regulation of some genes. Cohesins are deposited on chromosomes by chromatid cohesion (Scc) proteins, Scc2 Scc4, which form a heterodimeric cohesin deposition complex in budding yeast. Although Scc2/Scc4 is essential for deposition, its poorly understood. We demonstrate that dephosphorylation...
The cohesion of replicated sister chromatids promotes chromosome biorientation, gene regulation, DNA repair, and condensation. Cohesion is mediated by cohesin, which deposited on chromosomes a separate conserved loading complex composed Scc2 Scc4 in Saccharomyces cerevisiae. Although it known to be required, the role Scc2/Scc4 cohesin deposition remains enigmatic. phosphoprotein, although functions phosphorylation are unknown. We identified 11 phosphorylated residues mass spectrometry....
Abstract Cornelia de Lange Syndrome (CdLS) is the most common example of disorders cohesin complex, or cohesinopathies. There are a myriad clinical issues facing individuals with CdLS, particularly in neurodevelopmental system, which also have implications for parents and caretakers, involved professionals, therapists, schools. Basic research developmental cell biology on showing significant progress, improved understanding mechanisms possibility potential therapeutics. The following...